FastQCFastQC Report
Sun 2 Jul 2023
SRR4045293.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4045293.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61229192
Total Bases1.1 Gbp
Sequences flagged as poor quality0
Sequence length1-35
%GC77

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCC17120002.796051922422886No Hit
AGCGGTCGGCGTC14467902.3629088556321305No Hit
CGGGGGG14195922.3184888672056947No Hit
CCGCCGGC13953942.278968502475094No Hit
ACCCCCCTCGCGG12002281.9602218497346822No Hit
CGGGGGGC11626661.8988752946470369No Hit
ACCCCCCTCGCGGC11136841.8188775053572483No Hit
ATCCCGGACGA9332951.5242647657346189No Hit
TCCCCCGCGGGGGCGCGCCGGC7563141.2352179986304572No Hit
TCCCCCGCGGGGGCGCGCCGGCG6494491.0606852365453394No Hit
GCGGTCGGCGTC6144551.0035327593413286No Hit
AGGGGGGTCTCCCCCGCGGGGGCGCGCCGGC4928350.804902014712198No Hit
ACCCCCCTCGCG4826830.7883216881254941No Hit
AGGGGCCCGGGGCGGGGTCCGCCGG4448930.7266027616369656No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCCGC4441570.7254007206235875No Hit
CCCCCCGCGGGGGCGCGCCGGC4341970.7091339699534169No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCC4178500.6824359204348148No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCT3871320.6322670402052668No Hit
CGGGGGGGG3715700.6068510588870747No Hit
AGGGGGGTCTCCCCCGCGGGGGCGCGCCGGCG3698870.6041023699937115No Hit
ACCCCCCTCG3651940.5964377253255277No Hit
TTCCCCCGCGGGGGCGCGCCGGC3552720.5802330365555044No Hit
TCGCCCCAGCTGCGGCGGGCGTCGCG3396590.5547337616344832No Hit
TCACCCCCGCGGGGAATCCCCC3245760.5301000868997259No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCCG3198850.5224387086473393No Hit
ACCCCCGCGGGGAATCCCCC3111400.5081563055739818No Hit
TTCCCCCGCGGGGGCGCGCCGGCG3085200.5038773008796196No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCT3069530.5013180641024955No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCCGC2897190.4731713591778248No Hit
AGGGGCCCGGGGCGGGG2869510.46865063971446824No Hit
CCCCCCGCGGGGGCGCGCCGGCG2515170.41077955103506836No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCCG2338820.3819779297430546No Hit
AGGGGCCCGGGGCGGGGTCCGCCGGC2321050.37907571930722195No Hit
CCGCGAGGGGGGCCCGGGC2102690.34341299163314126No Hit
ATCCGGGTGCC2060970.3365992482801341No Hit
TCGGGGGG2031330.33175842006864964No Hit
TAGCGGTCGGCGTC1902200.3106688064738793No Hit
CCCGCGAGGGGGGCCCGGGC1863880.3044103538064001No Hit
GGGGCCACGCGCGCGTCCCCCG1821930.29755904667172484No Hit
AACCGGGCGGA1796610.2934237642724405No Hit
CCGCCGG1787820.291988174529561No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCG1776040.29006425562499666No Hit
AGCCCCTCTCCG1721120.2810946778458223No Hit
ATCCGAGTCACGGC1683050.27487705537580837No Hit
CTGTCCCCGCCGGCGGGC1671010.27291067306588007No Hit
TGCGGTCGGCGTC1669730.2727016224548578No Hit
TCGGGGGGC1666950.2722475906590438No Hit
ATCCCGGAC1631280.26642193808469655No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCGC1571640.2566814861773776No Hit
ACCCCTCCTCCCCG1569580.25634504535026365No Hit
TCACCCCCGCGGGGAATCCCCCGCGAGGGGGG1505380.24585985064117782No Hit
TTCCCGGCCAATGC1497970.24464964358830668No Hit
GCGCGTGTGGTG1460490.23852838038431082No Hit
CGGGGGGGGT1391600.22727721117077618No Hit
ATCCCGGACG1361230.22231715878269306No Hit
TGGGGCCACGCGCGCGTCCCCCG1326540.21665156058240978No Hit
GCGCGCGGGTCGGGGGGCGGGGCGGACTGT1312290.2143242393268884No Hit
TCTCGCTTCTGGCGCCAAGCGCCCGGCCGC1292850.21114928317198767No Hit
TTCGG1272730.20786326887998133No Hit
TGCCCCGCCGCGCG1206230.1970024363542148No Hit
TCTGACT1203550.19656473663738697No Hit
CTGTCCCCGCCGGCGG1190960.19450852789303508No Hit
TAGCCCCTCTCCG1185970.19369355715162792No Hit
TCTG1168830.19089423881340783No Hit
TAGCCCCTCTCCGG1158660.18923326638051993No Hit
CCCCGCCGCGCG1155900.18878250100050317No Hit
TCTCCCCCGCGGGGGCGCGCCGGC1143460.18675079037463047No Hit
CCCCCGCCGCGCG1131290.18476317636202028No Hit
ACCCCTCCTCCCCGCG1088030.1776979189926269No Hit
TCGGCGGCG1087450.17760319293450744No Hit
CCCCCGCGGGGGCGCGCCGGC1063320.1736622622751579No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGG1051260.17169261354943244No Hit
TTCTCGCTTCTGGCGCCAAGCGCCCGGCCGC1019030.16642878449220758No Hit
CGGGGGGGGTG1002500.16372909183580275No Hit
TCCGCCGGC983070.16055576888880063No Hit
CGGCGGCGG975690.15935046145962534No Hit
TCCCCGCCGCGCG972910.15889642966381134No Hit
ACCCCCGCGGGGAATCCCCCGCGAGGGGGG968310.15814515403045007No Hit
CGCGGGCCGGT955680.15608241245450372No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCC942250.15388901424666848No Hit
TCTCCCCCGCGGGGGCGCGCCGGCG933130.1523995286431348No Hit
GCCCCGCCGCGCG932700.1523293007034945No Hit
TGCGCGCGGGTCGGGGGGCGGGGCGGACTGT924690.1510211011767067No Hit
CTGTCCCCGCCGGCGGG923080.15075815470503023No Hit
CGGGGG905480.147883708803474No Hit
ATCCCGGA898010.1466637025032112No Hit
GTGTAATGGTTAGCACTCTGGACT890840.14549269243990678No Hit
ATCCGAGTCACG845820.1381399904803578No Hit
TCTCGCTTCTGGCGCCAAGCGCCCGGCCG802430.1310535014082825No Hit
CGCGGCGCCTCGCCTCGGCCGGCG801860.13096040855806165No Hit
TTCCCGGCCCATGC800760.1307807556892144No Hit
ATCCTGCCGACTACG794470.12975346792098774No Hit
TCGGCGGCGG790190.12905445494038204No Hit
ACCCCCGCGGGGAATCCCCCGCGAGGGGGGT788970.12885520357675143No Hit
AGCCCCTCTCCGG787230.12857102540239304No Hit
TCGGGGCCACGCGCGCGTCCCCC771370.1259807576751952No Hit
CTCGCCGGCCGAGGTGGGATCCCGAGGCCTC770060.1257668074404771No Hit
ATCCCGGACGAG752850.1229560566469667No Hit
CCCCCGCGGGGGCGCGCCGGCG752220.12285316454935417No Hit
AGCCCCTCTCCGGCC728840.11903472448240049No Hit
CCCGGGGCGGGGTCCGCCGG713740.11656858055549713No Hit
TACCCCCCTCGCGG712830.11641995863672348No Hit
TCACCCCCGCGGGGAATCCCCCGCGAGGGGGGT705520.11522608366283846No Hit
ATCTCGGTGGG686830.11217361810033358No Hit
TCTGA680920.11120839223225418No Hit
TCGGGGG667190.10896599778746059No Hit
TCGCCGCGGCCCGGGTTCGATTCCCGGTCAGGG655300.10702411359601154No Hit
CGGCGGCG653470.10672523655056562No Hit
TTCCCGGTCAGGGA650990.10632020099170998No Hit
ACCTCAGA648310.1058825012748821RNA PCR Primer, Index 40 (100% over 8bp)
ATCCCAGCGGTG636860.10401247823097193No Hit
TAGCCCCTCTCCGGCC633470.10345882075334262No Hit
GCGCAGCGGAAGCGTGCTGGGC632940.10337226073471621No Hit
AACCGGGCGGAAACA630860.10303255349180503No Hit
CCCCCCCGCGGGGGCGCGCCGGC615360.1005010812489572No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)