FastQCFastQC Report
Sun 2 Jul 2023
SRR4045294.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4045294.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29753944
Total Bases597.9 Mbp
Sequences flagged as poor quality0
Sequence length1-35
%GC75

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCGCGGGGGCGCGCCGGC6972962.3435414142071385No Hit
TCCCCCGCGGGGGCGCGCCGGC6070792.040331191051512No Hit
GCGGTCGGCGTC5915331.9880826555296334No Hit
AGCGGTCGGCGTC5421431.8220878549747892No Hit
CGGGGGGC4899831.6467833642491227No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCC4405391.4806070751494322No Hit
ACCCCCCTCGCGGC4166361.4002715068630902No Hit
CCCCCGCGGGGGCGCGCCGGC4072931.3688706277056917No Hit
CCGCCGGC3310751.1127096293519945No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCCG3252031.0929744305494424No Hit
ATCCCGGACGA3021571.0155191526877916No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCT2741910.9215282518512504No Hit
AGGGGGGTCTCCCCCGCGGGGGCGCGCCGGC2657680.8932193997541973No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCCG2609720.8771005282526578No Hit
CGGGGGG2389320.803026314763515No Hit
TCCCCCGCGGGGGCGCGCCGGCG1967210.6611594079763006No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCT1897460.6377171376003127No Hit
TCGCCGGCCGAGGTGGGATCCCGAGGCC1792690.6025049990011409No Hit
ACCCCCCTCGCGG1560740.5245489471916731No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCC1416880.47619905448501215No Hit
TTCCCCCGCGGGGGCGCGCCGGC1345920.4523501153326093No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCG1300560.43710507756551537No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCCGC1259930.42344974501531635No Hit
TCTCGCTTCTGGCGCCAAGCGCCCGGCCG1202550.40416490667590155No Hit
CTCGCTTCTGGCGCCAAGCGCCCGG1178040.39592734327926415No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCC1142360.38393565572349No Hit
ACCCCCCTCGCG1140310.3832466714328695No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCCGC1127740.37902202141672375No Hit
CCCCGCCCCTTGCCTCTCGGC1117420.3755535736707712No Hit
AGGGGGGTCTCCCCCGCGGGGGCGCGCCGGCG1110800.373328658546914No Hit
CCCCGCGGGGGCGCGCCGGC1069330.3593910104825095No Hit
CCGGGGCGGG1046180.35161052934696657No Hit
TCGCCCCAGCTGCGGCGGGCGTCGCG1017680.34203196725785323No Hit
ATCCCGGACG1011740.34003559326454336No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCC1000150.33614031134830397No Hit
ATGCGTGCATTTATCAGA995130.33445314006102855No Hit
TGCGGTCGGCGTC988000.3320568190892609No Hit
TCCCCGCGGGGGCGCGCCGGC978000.32869592011062465No Hit
TCGCTTCTGGCGCCAAGCGCCCGG971550.32652814026940424No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCG929120.3122678459030507No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCG912290.30661145292200587No Hit
ACCCCCGCGG793270.2666100332782773No Hit
TTCTCGCTTCTGGCGCCAAGCGCCCGGCCG791130.26589080089684913No Hit
AGCCCCTCTCCGG758370.25488049584283684No Hit
CCCCGCCCCTTGCCTCTCGGCG755890.2540469928961351No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGC734870.24698238324304167No Hit
TCTCCCCCGCGGGGGCGCGCCGGC721030.2423308990566091No Hit
GCGGGGCGGACTGT716750.2408924342937528No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTT703520.23644596494501705No Hit
CCCCGCCGCGCG698380.234718462869998No Hit
CCCCCCCGCGGGGGCGCGCCGGC697760.23451008713332258No Hit
ATGCGTGCATTTATCAGAT671030.22552640416342787No Hit
ATCCGAGTCACGGC669800.2251130135890556No Hit
ATCCGAGTCACG665400.22363421803845568No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCG663020.22283432408154025No Hit
ACCCCCCTCG637610.21429427977682555No Hit
AGGGGCCCGGGGCGGGGTCCGCCGGC633570.21293647658945652No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCC624740.2099688027913207No Hit
GGGGGGGTCTCCCCCGCGGGGGCGCGCCGGC581660.19549004999135575No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCC578950.19457924636814536No Hit
ACCCCCGCGGGGAATCCCCC574650.19313405980733175No Hit
TCGCCGGCCGAGGTGGGATCCCGAGGCCTC573040.19259295507177132No Hit
GGCGCGCCGGC572510.1924148274259036No Hit
TTCCCCCGCGGGGGCGCGCCGGCG571030.19191741437706544No Hit
AGGGGCCCGGGGCGGGGTCCGCCGG557590.18740036614977834No Hit
TCTG547980.18417054223130888No Hit
TCGCTTCTGGCGCCAAGCGCCCGGC540510.18165995069426763No Hit
TCGCCGGCCGAGGTGGGATCCCGAGGCCT540500.18165658979528898No Hit
TCTCGCTTCTGGCGCCAAGCGCCCGG518370.174218920355567No Hit
TCGACTGCATAATTTGTGGTAGTGGGGG518250.17417858956782334No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCT500940.16836087343580403No Hit
CCCCCCGCGGGGGCGCGCCGGCG493140.16573937223246774No Hit
CTCGCTTCTGGCGCCAAGCGCCCG491690.1652520418805655No Hit
CCGCGAGGGGGGCCCGGGC488240.16409253173293598No Hit
ATCCCGGAC477970.16064088848187655No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGC477510.1604862871288593No Hit
TCACCCCCGCGGGGAATCCCCC473430.1591150403455757No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGC472770.1588932210129857No Hit
GGGGCCACGCGCGCGTCCCCCG459820.15454085683565177No Hit
CGGGGGGGG450700.15147571696713552No Hit
TAGCCCCTCTCCGG438400.14734181122341294No Hit
TCCCCGCCCCTTGCCTCTCGGC432020.145197557675043No Hit
TCTCGCTTCTGGCGCCAAGCGCCCGGCCGC430550.1447035055251835No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTT430420.14465981383846122No Hit
CCCCCGCCGCGCG429520.14435733293038394No Hit
TTCTCGCTTCTGGCGCCAAGCGCCCGG427940.14382631089175943No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGA424320.1426096654614931No Hit
AGCCCCTCTCCG423810.14243825961358264No Hit
GCCCCGCCGCGCG414150.13919163120022005No Hit
TCGCTTCTGGCGCCAAGCGCCCG406670.13667767876420014No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCT406280.13654660370403332No Hit
ACCCCTCCTCCCCGCG404670.1360054989684729No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCGC402520.13528290568806609No Hit
ACGA392910.13205308176959665No Hit
GGGGCCACGCGCGCGTCCCCC383710.12896105470925132No Hit
AGGGCGCCCTGGAATGGGT382400.12852077694304997No Hit
ATCTCGCTGGGG381290.12814771715642134No Hit
ATGCGTGCATTTATCAGATC374640.12591271933562825No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCC370280.12444736738094285No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCG368150.12373149589849332No Hit
CGCGGCGCCTCGCCTCGGCCGGCG364710.12257534664984246No Hit
CCCGCGAGGGGGGCCCGGGC364550.12252157226618426No Hit
CCCCGCCCCTTGCCTCTCGGCGCCCC362870.1219569412377734No Hit
ACTCGACTGCATAATTTGTGGTAGTGGGGGA355550.11949676318541165No Hit
TGCCCCGCCGCGCG355210.11938249262013802No Hit
TCTGACT347520.11679796130556674No Hit
TGCGTGCATTTATCAGA347350.11674082602292993No Hit
TCGGGGGGC344620.11582330060176224No Hit
AGGGGCCCGGGGCGGGG341910.11491249697855183No Hit
TTCCCGGTCAGGG341120.11464698595923956No Hit
CTCCCCCGCGGGGGCGCGCCGGC338540.1137798740227514No Hit
TCCCCGCCCCTTGCCTCTCGGCG335870.11288251399545553No Hit
ACCCTACGCCGGCAGGCGCGGGT335600.11279176972303236No Hit
TCCCCGCCGCGCG335320.11269766455163054No Hit
CGGGGGGGGT325090.10925946489648566No Hit
CCCCCGCGGGGGCGCGCCGGCG323820.10883263072619885No Hit
ACCCCTCCTCCCCG320590.10774706035609934No Hit
ATCCTGCCG319380.10734039157968436No Hit
ACCCCCGCGGG315700.10610358075554623No Hit
TCGCCGCGGCCCGGGTTCGATTCCCGGTCAGGG314940.10584815243316988No Hit
ATCCCAGCGG313700.10543140095981897No Hit
ATCCGGGTGCC313700.10543140095981897No Hit
TTCCCGGCCAATGC313010.10519949893029307No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCT311330.10463486790188217No Hit
TTCCCGGGC310090.1042181164285313No Hit
TCACCCCCGCGGGGAATCCCCCGCGAGGGGGGT300910.10113281116614321No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGC297950.1001379850684669No Hit
AGAGCACTGGT297760.1000741279878728No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)