FastQCFastQC Report
Mon 3 Jul 2023
SRR4050168.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4050168.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32402573
Total Bases1.6 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGG16472995.083852445915329No Hit
TGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGG9149382.8236584792201533No Hit
AGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGA6287921.9405619424111782No Hit
TCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGAA4741931.4634424247728721No Hit
TTCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGA3296401.0173266178584028No Hit
TGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGA3077020.9496221179719276No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAA2427370.7491287806064043No Hit
TCGACCGATCCTGACTGTAGGCACCATCAATAGATCGGAAGAGCACACGT2414360.7451136673621567No Hit
ACGAATAAGGCGTCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT2050780.6329065287500472No Hit
CAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACAC1965170.6064857874095368No Hit
TATAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATAGATCGGA1963680.6060259473838697No Hit
ACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGGAAGAGCAC1755380.5417409290305433No Hit
ACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACA1600910.49406878891994166No Hit
TAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCG1475070.4552323668864198No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGATCTGTAGGCACCATCAATAGA1407180.43428032705921227No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGATACTGTAGGCACCATCAATAG1316620.40633192925759326No Hit
ACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGAAGA1295310.39975529103815305No Hit
ATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGGAAGAGCACACG1228500.3791365580751874No Hit
ATATAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATAGATCGG1217540.3757541106380657No Hit
AGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATAGATCGGAAGA1081420.3337451010449078No Hit
AAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACAC1042930.3218664147442859No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1017810.3141139439759923Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGGAAGAGCACA1013190.3126881312789574No Hit
ATGATTAATAGGGACGGTCGGGGGCCTGTAGGCACCATCAATAGATCGGA972080.30000086721508196No Hit
ATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATAGATCGGAAGAG948500.29272366734579996No Hit
TTTGCTCGAATATATTAGCATGGAATCTGTAGGCACCATCAATAGATCGG922260.2846255450145888No Hit
TCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC894250.27598116976698117No Hit
AAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGGAAGAGCACA890390.2747899063447832No Hit
ATAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATAGATCGGAA866420.26739234566341386No Hit
AATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGGAAGAGCACAC866260.26734296686871134No Hit
TTACCTATACTCTACCGTCAGGGTTGACTGTAGGCACCATCAATAGATCG864930.26693250563774673No Hit
AGAGGTGCACAATCGACCGATCCTGTAGGCACCATCAATAGATCGGAAGA850260.26240508739846063No Hit
TTTCGGTGCCCGAGTTGTAATTTGCTGTAGGCACCATCAATAGATCGGAA842410.25998244028336887No Hit
TCACGTTCTAGCATTCAAGGTCCCGCTGTAGGCACCATCAATAGATCGGA833780.2573190715441024No Hit
ATACGAATAAGGCGTCCTGTAGGCACCATCAATAGATCGGAAGAGCACAC832900.2570474881732386No Hit
AAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCG801230.2472735729968111No Hit
TTGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCG800680.24710383339002123No Hit
AATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACACG709400.2189332310122409No Hit
AGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGGAA705180.2176308653019623No Hit
ACTGCCAGCTGGGACTGAGGACTGCGACGCTGTAGGCACCATCAATAGAT698960.2157112646579023No Hit
TAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATAGATCGGAAG689910.21291827658254178No Hit
ATCCTGTCTGTGACGCCACTGTAGGCACCATCAATAGATCGGAAGAGCAC667340.20595278035481934No Hit
TAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACAC653270.20161053259566764No Hit
AGAGGTGCACAATCGACCGATCCTCTGTAGGCACCATCAATAGATCGGAA636380.19639798357988422No Hit
TCACGTTCTAGCATTCAAGGTCCCTGTAGGCACCATCAATAGATCGGAAG624180.19263285048381806No Hit
AAATATAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATAGATC614580.18967012280166762No Hit
ACTATGCCGACTAGGGATCGGGTGGTGCTGTAGGCACCATCAATAGATCG588370.1815812589944632No Hit
ATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATAGATCGGAAG571530.1763841408520243No Hit
TTTCGGTGCCCGAGTTGTAATTTCTGTAGGCACCATCAATAGATCGGAAG560990.17313131275099666No Hit
AACTTTTCGAATCGCATGGCCCTGTAGGCACCATCAATAGATCGGAAGAG555450.17142157198442234No Hit
TTCACGTTCTAGCATTCAAGGTCCCGCTGTAGGCACCATCAATAGATCGG543070.16760088774431586No Hit
TAGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGG537400.16585102670704577No Hit
ATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT525250.16210132448432413No Hit
CCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGAA521260.16086994079143035No Hit
TCAATATAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATAGAT519640.16036998049506748No Hit
TATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATAGATCGGAAGA514450.1587682558419049No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAAT502260.15500620892050762No Hit
ATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATAGATCGGAAGAGC490140.15126576522179272No Hit
TACGAATAAGGCGTCCTGTAGGCACCATCAATAGATCGGAAGAGCACACG467470.14426940724738124No Hit
ACCTATACTCTACCGTCAGGGTTGACTGTAGGCACCATCAATAGATCGGA453170.13985617747084467No Hit
GAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGGA451460.13932844160246163No Hit
ACGTTCTAGCATTCAAGGTCCCGCTGTAGGCACCATCAATAGATCGGAAG435100.13427945984413028No Hit
AGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAG434140.13398318707591525No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGATCTGTAGGCACCATCAATAGAT424860.13111921698316983No Hit
TAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGGAAGAGCACA419990.12961624991941226No Hit
TGAGGTGCACAATCGACCGATCCTGTAGGCACCATCAATAGATCGGAAGA419010.1293138048018594No Hit
TTTACCTATACTCTACCGTCAGGGTTGACTGTAGGCACCATCAATAGATC418560.1291749269417586No Hit
TTCACGTTCTAGCATTCAAGGTCCCTGTAGGCACCATCAATAGATCGGAA413190.1275176511445557No Hit
TGAGGTGCACAATCGACCGATCCTCTGTAGGCACCATCAATAGATCGGAA390050.12037624296070562No Hit
ATGATTAATAGGGACGGTCGGGGGCACTGTAGGCACCATCAATAGATCGG351560.10849755666008376No Hit
TGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGGAAGAG351040.1083370755773006No Hit
TCAATATAGATGGATACGAATCTGTAGGCACCATCAATAGATCGGAAGAG350460.10815807744650402No Hit
GCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGAA344270.10624773532645078No Hit
TCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGA337610.10419234299695891No Hit
TCACGTTCTAGCATTCAAGGTCCTGTAGGCACCATCAATAGATCGGAAGA335810.10363683155655572No Hit
AATACTGCCAGCTGGGACTGAGGACTGCGACGCTGTAGGCACCATCAATA328220.10129442498285551No Hit
AGCTGGGACTGAGGACTGCGACGCTGTAGGCACCATCAATAGATCGGAAG325650.1005012780929465No Hit
TTATAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATAGATCGG324800.10023895324608945No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)