FastQCFastQC Report
Mon 3 Jul 2023
SRR4050175.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4050175.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences76469638
Total Bases3.8 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA13508771.766553413002949Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ACGTTCTAGCATTCAAGGTCCTGTAGGCACCATCAATAGATCGGAAGAGC5716880.7476012898086427No Hit
CCTGATTCAAGCTCAGGTACTGTAGGCACCATCAATAGATCGGAAGAGCA4445870.5813902244443736No Hit
TTGCCCGAGTTGTAATTTCTGTAGGCACCATCAATAGATCGGAAGAGCAC4150090.5427108207312293No Hit
TCACGTTCTAGCATTCAAGGTCCTGTAGGCACCATCAATAGATCGGAAGA3937470.5149063213820889No Hit
GGGAGAGCAGCCCCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACG2786370.3643759893305628No Hit
GGGAAAAAAACAAATCCTGTAGGCACCATCAATAGATCGGAAGAGCACAC2201920.2879469626886425No Hit
TGGGAGACGCTCAAACCTGTAGGCACCATCAATAGATCGGAAGAGCACAC2171330.28394668221131114No Hit
GGGAGAGCAGCCCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT2078790.27184514722039094No Hit
ACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTC1586330.20744573159872942Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ATGTACACGGAGTCGCTGTAGGCACCATCAATAGATCGGAAGAGCACACG1505620.19689121583130811No Hit
TGCCCGAGTTGTAATTTCTGTAGGCACCATCAATAGATCGGAAGAGCACA1504050.19668590558778376No Hit
TGGGAGATGATCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT1484720.19415810494617486Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTGCCCGAGTTGTAATTTGCTGTAGGCACCATCAATAGATCGGAAGAGCA1476260.19305178350654673No Hit
GACTCCATGGCCAAGTTGGCTGTAGGCACCATCAATAGATCGGAAGAGCA1464710.1915413801226573No Hit
TTCACGTTCTAGCATTCAAGGTCCTGTAGGCACCATCAATAGATCGGAAG1403700.18356305021347166No Hit
GGGAGAGCAGCCCCCCCTGTAGGCACCATCAATAGATCGGAAGAGCACAC1352160.1768231203082196No Hit
ATGTACACGGAGTCGAGCACCCGCACTGTAGGCACCATCAATAGATCGGA1330510.17399193128127532No Hit
GGGAGACGCTCAAACCTGTAGGCACCATCAATAGATCGGAAGAGCACACG1194540.15621101802521936No Hit
ACCAAAGAAGAGAAAAACTGTAGGCACCATCAATAGATCGGAAGAGCACA1191850.15585924442325724No Hit
ATGTATTTATTAGACTGTAGGCACCATCAATAGATCGGAAGAGCACACGT1177920.15403760640268757No Hit
GGGAGAACCCCCCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT1154870.1510233381776961No Hit
TCAGATTTAGGCTCTGATACTGTAGGCACCATCAATAGATCGGAAGAGCA1146020.14986601610432626No Hit
TGGGAGAGCAGCCCCCCTGTAGGCACCATCAATAGATCGGAAGAGCACAC1143760.1495704739703358No Hit
TGGTTGTTTGGCCGAGCGGCTGTAGGCACCATCAATAGATCGGAAGAGCA1126870.14736175421675202No Hit
GAAAGACTAACAAGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT1080680.14132144838975177No Hit
TGCGACTGTTAATCGCACTGTAGGCACCATCAATAGATCGGAAGAGCACA1024020.1339119717030699No Hit
CTGCCCGAGTTGTAATTTCTGTAGGCACCATCAATAGATCGGAAGAGCAC1021360.13356412122678024No Hit
GGGAGATGATCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT995380.1301666943944471Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGGAAACAGCCCAGACTGTAGGCACCATCAATAGATCGGAAGAGCACACG990050.12946968573331027No Hit
GGAAAAAAACAAATCCTGTAGGCACCATCAATAGATCGGAAGAGCACACG978050.12790043546433422No Hit
GGGAGAACCCCCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC977650.12784812712203503No Hit
TTTACTTATTCAATGAAGCGGAGCTGTAGGCACCATCAATAGATCGGAAG974720.12746496851469336No Hit
TACCCGCAACGCGAATGCTGTAGGCACCATCAATAGATCGGAAGAGCACA968140.12660449628387152No Hit
TGTGGCGTCGCTGAACCATAGCTGTAGGCACCATCAATAGATCGGAAGAG967940.12657834211272193No Hit
TGGGAGAACCTACAGCCTGTAGGCACCATCAATAGATCGGAAGAGCACAC939150.1228134491757369No Hit
TGAAGAAATTCAACCAAGCTGTAGGCACCATCAATAGATCGGAAGAGCAC931740.1218444371346442No Hit
TGACAACAGCCAAGACTGTAGGCACCATCAATAGATCGGAAGAGCACACG930450.12167574273072929No Hit
GGGAAACAGCCCAGACTGTAGGCACCATCAATAGATCGGAAGAGCACACG906610.1185581655296969No Hit
CCACGTTCTAGCATTCAAGGTCCTGTAGGCACCATCAATAGATCGGAAGA892990.1167770664744091No Hit
GGAAAAGAAACCAACCTGTAGGCACCATCAATAGATCGGAAGAGCACACG886280.11589959403234No Hit
GCCAAAGAAGAGAAAAACTGTAGGCACCATCAATAGATCGGAAGAGCACA878730.11491227407144258No Hit
TTTTACTTATTCAATGAAGCGGAGCTGTAGGCACCATCAATAGATCGGAA823610.10770418450261265No Hit
TGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGA816190.10673386475296247No Hit
TGGAAAAGAAACCAACCTGTAGGCACCATCAATAGATCGGAAGAGCACAC802020.10488084172701327No Hit
TGGGAGATGATCCTTCCGCCTGTAGGCACCATCAATAGATCGGAAGAGCA789490.1032422829044908No Hit
TCATATTCTAGCGTTAAAGTTCTGTAGGCACCATCAATAGATCGGAAGAG785140.10267342968198699No Hit
TGGGAGATGATCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC772750.10105317877926923No Hit
ACGGAGTCGAGCACCCGCACTGTAGGCACCATCAATAGATCGGAAGAGCA770850.10080471415334802No Hit
CCTGATTCAAGCTCAGGTCTGTAGGCACCATCAATAGATCGGAAGAGCAC768960.1005575572359843No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)