FastQCFastQC Report
Tue 4 Jul 2023
SRR4050177.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4050177.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences79747539
Total Bases4 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1003558612.584195231403944Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ATGTACACGGAGTCGAGCACCCGCACTGTAGGCACCATCAATAGATCGGA14375671.8026474773096133No Hit
ATGTACACGGAGTCGAGCACCCGCAACGCGAATGCTGTAGGCACCATCAA11347961.4229856046090652No Hit
CCTGATTCAAGCTCAGGTACTGTAGGCACCATCAATAGATCGGAAGAGCA7356570.9224823853184986No Hit
ACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTC7219050.9052379660267635Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ATGTATTTATTAGACTGTAGGCACCATCAATAGATCGGAAGAGCACACGT6239760.782439192261469No Hit
ATGTACACGGAGTCGCTGTAGGCACCATCAATAGATCGGAAGAGCACACG5537050.6943223664870711No Hit
TTGCCCGAGTTGTAATTTCTGTAGGCACCATCAATAGATCGGAAGAGCAC4927850.6179312944064643No Hit
TTGTGGCGTCGCTGAACCATAGCTGTAGGCACCATCAATAGATCGGAAGA3014060.37795022113472365No Hit
TCTACCAAAGGCATGCCTGTTTGAGTGTCATTCTGTAGGCACCATCAATA2981010.3738058926182035No Hit
TTGTGGCGTCGCTGAACCATACTGTAGGCACCATCAATAGATCGGAAGAG2778430.34840322784130057No Hit
ACGTTCTAGCATTCAAGGTCCTGTAGGCACCATCAATAGATCGGAAGAGC2407090.3018387815077278No Hit
TCACGTTCTAGCATTCAAGGTCCTGTAGGCACCATCAATAGATCGGAAGA2249310.28205384494686414No Hit
TGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC2044870.2564179441324202Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCAGATTTAGGCTCTGATACTGTAGGCACCATCAATAGATCGGAAGAGCA1946550.2440890370297195No Hit
ATGTATTTATTAGATCTGTAGGCACCATCAATAGATCGGAAGAGCACACG1895590.23769887118397473No Hit
GGGAAAAAAACAAATCCTGTAGGCACCATCAATAGATCGGAAGAGCACAC1752540.21976101356557223No Hit
TGTGGCGTCGCTGAACCATAGCTGTAGGCACCATCAATAGATCGGAAGAG1713650.21488437404946129No Hit
TATGTATTTATTAGACTGTAGGCACCATCAATAGATCGGAAGAGCACACG1631830.20462449631204294No Hit
TTTATATGTATAAAAATAGAGGTACTGTAGGCACCATCAATAGATCGGAA1411530.17699981939254578No Hit
ACGGAGTCGAGCACCCGCACTGTAGGCACCATCAATAGATCGGAAGAGCA1365070.17117393428278707No Hit
TCATATTCTAGCGTTAAAGTTCTGTAGGCACCATCAATAGATCGGAAGAG1319900.16550980965067774No Hit
ACGGAGTCGAGCACCCGCAACGCGAATGCTGTAGGCACCATCAATAGATC1311950.16451291368376897No Hit
TGTGGCGTCGCTGAACCATACTGTAGGCACCATCAATAGATCGGAAGAGC1302800.16336554285393057No Hit
TTCACGTTCTAGCATTCAAGGTCCTGTAGGCACCATCAATAGATCGGAAG1294790.16236112314387532No Hit
ATATTCTAGCGTTAAAGTTCTGTAGGCACCATCAATAGATCGGAAGAGCA1264370.1585465853686093No Hit
CTGTAGGCACCATCAATAACTGTAGGCACCATCAATAGATCGGAAGAGCA1192190.1494955223633923No Hit
TACCCGCAACGCGAATGCTGTAGGCACCATCAATAGATCGGAAGAGCACA1145940.14369597035464632No Hit
TCGAGCACCCGCAACGCGAATGCTGTAGGCACCATCAATAGATCGGAAGA1092930.13704874328473007No Hit
TATGTACACGGAGTCGCTGTAGGCACCATCAATAGATCGGAAGAGCACAC1071690.1343853382108757No Hit
TTGCCCGAGTTGTAATTTGCTGTAGGCACCATCAATAGATCGGAAGAGCA1040450.1304679759459411No Hit
TGGGAGACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACT1026570.12872748336472176Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TGGGAGATGATCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT1023090.12829110626222584Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGTACACGGAGTCGAGCACCCGCACTGTAGGCACCATCAATAGATCGGAA989060.12402388994097986No Hit
GGGAGAGCAGCCCCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACG963300.12079369621675723No Hit
CTGCCCGAGTTGTAATTTCTGTAGGCACCATCAATAGATCGGAAGAGCAC957330.12004508377368235No Hit
CCTACCAAAGGCATGCCTGTTTGAGTGTCATTCTGTAGGCACCATCAATA957030.12000746505794993No Hit
TGCCCGAGTTGTAATTTCTGTAGGCACCATCAATAGATCGGAAGAGCACA917080.11499790607958446No Hit
TGGGAGATGATCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC903750.11332638114387454No Hit
ACCCGCAACGCGAATGCTGTAGGCACCATCAATAGATCGGAAGAGCACAC892730.11194452031930416No Hit
CATATTCTAGCGTTAAAGTTCTGTAGGCACCATCAATAGATCGGAAGAGC885270.11100906825475831No Hit
TACACGGAGTCGAGCACCCGCACTGTAGGCACCATCAATAGATCGGAAGA865610.1085437884170946No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCACTCA862420.10814377607314002Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)
TGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGA818260.10260630111732978No Hit
TCAGGTCGAGGTCCTGATCCTGTAGGCACCATCAATAGATCGGAAGAGCA798310.10010465652112474No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)