FastQCFastQC Report
Mon 3 Jul 2023
SRR4050178.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4050178.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28663385
Total Bases1.4 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA6417122.238786521550054Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ATGTACACGGAGTCGAGCACCCGCAACGCGAATGCTGTAGGCACCATCAA4708351.6426357180074858No Hit
ATGTATTTATTAGACTGTAGGCACCATCAATAGATCGGAAGAGCACACGT1333320.4651648784677735No Hit
ATGTACACGGAGTCGCTGTAGGCACCATCAATAGATCGGAAGAGCACACG1258570.43908631168300605No Hit
TGGGAGACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACT835830.2916019862971522Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ACGGAGTCGAGCACCCGCAACGCGAATGCTGTAGGCACCATCAATAGATC713520.248930822371468No Hit
GGGAGAACCCCCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC691010.24107759777848986No Hit
ACGTTCTAGCATTCAAGGTCCTGTAGGCACCATCAATAGATCGGAAGAGC664850.23195097159669034No Hit
GGGAGAGCAGCCCCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACG604020.21072877470682547No Hit
TGGGAGATGATCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT597910.20859713533485316Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGG558330.1947885778319623No Hit
GGGAGAGCAGCCCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT525400.18330005336075975No Hit
CCTGATTCAAGCTCAGGTACTGTAGGCACCATCAATAGATCGGAAGAGCA519710.1813149423907888No Hit
ATGTATTTATTAGATCTGTAGGCACCATCAATAGATCGGAAGAGCACACG500290.17453974818396362No Hit
GGGAGAACCCCCCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT478830.1670528445959889No Hit
GGGAGATGATCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG431250.15045326991211957Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCACGTTCTAGCATTCAAGGTCCTGTAGGCACCATCAATAGATCGGAAGA425180.1483355856260522No Hit
GGGAGATGATCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT422480.14739361732747197Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGAGACAACACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG400530.13973576393716236Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGAGAACCCCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT379650.13245120909480856Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTC347790.12133598317156191Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TGGGAGACAACACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT338130.11796582992553042Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAA331150.11553066743512673No Hit
ATTTATTAGACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGA318630.11116272554689546Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGT318370.11107201748851364Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
ATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATAGATCGGAAG316070.11026960004898234No Hit
TATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATAGATCGGAAGA311700.10874500691387289No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAAT309910.10812051681962893No Hit
TGGGAGAGCAGCCCCCCTGTAGGCACCATCAATAGATCGGAAGAGCACAC303980.10605167533422866No Hit
AGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGA300800.10494224600478973No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAA286840.10007192102398234No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)