FastQCFastQC Report
Mon 3 Jul 2023
SRR4050179.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4050179.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences71112266
Total Bases3.5 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA25198043.5434168276960825Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ATGTATTTATTAGACTGTAGGCACCATCAATAGATCGGAAGAGCACACGT12139841.707137275023693No Hit
TATGACTCTTAATCATACTGTAGGCACCATCAATAGATCGGAAGAGCACA5595920.7869134700334257No Hit
TCAGATTTAGGCTCTGATACTGTAGGCACCATCAATAGATCGGAAGAGCA5586970.7856548967234429No Hit
TTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAATA4417330.6211769429482109No Hit
TTGATGATTCATAATAACTTTTCGAATCGCACTGTAGGCACCATCAATAG4271300.6006418077016418No Hit
TTGCCCGAGTTGTAATTTCTGTAGGCACCATCAATAGATCGGAAGAGCAC4035570.5674928148120044No Hit
AAAGATTGGAAATCTTTTGCTGTAGGCACCATCAATAGATCGGAAGAGCA3907890.5495381064076906No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAA3811090.5359258274796082No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAAT3484520.49000266705043544No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAA3380670.47539899797314855No Hit
ATTTATTAGACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGA3216540.4523185915633739Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCTGACTTCTAATCAGACTGTAGGCACCATCAATAGATCGGAAGAGCACA2935880.41285142003490655No Hit
CAAGACTGTAAATCTTGAGCTGTAGGCACCATCAATAGATCGGAAGAGCA2691990.3785549457810837No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCACTGTAGGCACCATCAAT2528190.35552094486765473No Hit
TATGTATTTATTAGACTGTAGGCACCATCAATAGATCGGAAGAGCACACG2129280.299425137148632No Hit
TGGGAGATGATCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT2122100.29841546604632174Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATAGATCGGAAG1980920.2785623509733187No Hit
CCTGATTCAAGCTCAGGTACTGTAGGCACCATCAATAGATCGGAAGAGCA1913630.26909984840027457No Hit
GGGAAAAAAACAAATCCTGTAGGCACCATCAATAGATCGGAAGAGCACAC1585050.22289403631154153No Hit
TATGACTCTTAATCATAAGGCTGTAGGCACCATCAATAGATCGGAAGAGC1544040.21712709872021232No Hit
ATGTATTTATTAGATCTGTAGGCACCATCAATAGATCGGAAGAGCACACG1469750.2066802371337738No Hit
ACGTTCTAGCATTCAAGGTCCTGTAGGCACCATCAATAGATCGGAAGAGC1461920.20557916126593406No Hit
TTAAGGCAAAAGATTGGAAATCTTTTGCTGTAGGCACCATCAATAGATCG1320330.18566839087928938No Hit
TATGACTCTTAATCATAAGGTTCTGTAGGCACCATCAATAGATCGGAAGA1157660.16279329363516556No Hit
GGGAGATGATCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG1146320.16119863203346663Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGCGACTGTTAATCGCACTGTAGGCACCATCAATAGATCGGAAGAGCACA1134920.15959553306879576No Hit
ATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATAGATCGGAAGAG1063320.14952694658893306No Hit
TATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATAGATCGGAAGA1061250.14923585756640073No Hit
ATCTGTTGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAAC1052680.14803072088857358Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAAT1038550.14604372190867887No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCACTGTAGGCACCATCAATA956550.1345126591803445No Hit
AAATAGAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAA941320.1323709752126307Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGGAGATGATCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT931410.13097740409509662Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TAAGGCAAAAGATTGGAAATCTTTTGCTGTAGGCACCATCAATAGATCGG926670.1303108524203124No Hit
TCAGTCTCATAATCTGAAGGCTGTAGGCACCATCAATAGATCGGAAGAGC907510.12761652117793573No Hit
ACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTC907320.1275898028618579Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TGGAAAAGAAACCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT768400.10805449512746508Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATGCCTTGCTAAGGCATTGCTGTAGGCACCATCAATAGATCGGAAGAGCA720330.10129476115976954No Hit
TTAAGATCTGTTGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC717700.10092492341616564No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)