FastQCFastQC Report
Wed 27 Sept 2023
SRR4050180.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4050180.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences66712527
Total Bases3.3 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA22773393.413660226062191Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ATGTATTTATTAGACTGTAGGCACCATCAATAGATCGGAAGAGCACACGT11305311.694630755030461No Hit
TATGACTCTTAATCATACTGTAGGCACCATCAATAGATCGGAAGAGCACA5222640.7828574684331775No Hit
TCAGATTTAGGCTCTGATACTGTAGGCACCATCAATAGATCGGAAGAGCA5184690.777168881640475No Hit
TTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAATA4121190.617753544248144No Hit
TTGATGATTCATAATAACTTTTCGAATCGCACTGTAGGCACCATCAATAG3986260.5975279575303751No Hit
AAAGATTGGAAATCTTTTGCTGTAGGCACCATCAATAGATCGGAAGAGCA3680840.5517464508577227No Hit
TTGCCCGAGTTGTAATTTCTGTAGGCACCATCAATAGATCGGAAGAGCAC3666340.5495729460225663No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAA3523270.5281271986594063No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAAT3240340.485716872934524No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAA3102630.4650745728759458No Hit
ATTTATTAGACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGA2920770.4378143253365294Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCTGACTTCTAATCAGACTGTAGGCACCATCAATAGATCGGAAGAGCACA2732940.4096591933925693No Hit
CAAGACTGTAAATCTTGAGCTGTAGGCACCATCAATAGATCGGAAGAGCA2514710.37694719613904No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCACTGTAGGCACCATCAAT2321770.3480260911117937No Hit
TATGTATTTATTAGACTGTAGGCACCATCAATAGATCGGAAGAGCACACG1970400.2953568225649734No Hit
TGGGAGATGATCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT1961270.29398826400325084Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATAGATCGGAAG1852510.27768547877072625No Hit
CCTGATTCAAGCTCAGGTACTGTAGGCACCATCAATAGATCGGAAGAGCA1773270.26580764958880965No Hit
GGGAAAAAAACAAATCCTGTAGGCACCATCAATAGATCGGAAGAGCACAC1473060.22080710568796172No Hit
TATGACTCTTAATCATAAGGCTGTAGGCACCATCAATAGATCGGAAGAGC1421970.21314887382395215No Hit
ACGTTCTAGCATTCAAGGTCCTGTAGGCACCATCAATAGATCGGAAGAGC1370920.20549663783534988No Hit
ATGTATTTATTAGATCTGTAGGCACCATCAATAGATCGGAAGAGCACACG1366510.20483559257169198No Hit
TTAAGGCAAAAGATTGGAAATCTTTTGCTGTAGGCACCATCAATAGATCG1230450.18444062237366604No Hit
TATGACTCTTAATCATAAGGTTCTGTAGGCACCATCAATAGATCGGAAGA1075570.16122459279649232No Hit
GGGAGATGATCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG1056940.15843201382552935Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGCGACTGTTAATCGCACTGTAGGCACCATCAATAGATCGGAAGAGCACA1056480.15836306125834507No Hit
ATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATAGATCGGAAGAG991720.14865573897388115No Hit
TATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATAGATCGGAAGA986390.14785678857585474No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAAT963520.14442864681171497No Hit
ATCTGTTGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAAC938810.14072469477883817Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTTGATGATTCATAATAACTTTTCGAATCGCACTGTAGGCACCATCAATA888210.13313991238856834No Hit
AAATAGAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAA882710.13231547952006076Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TAAGGCAAAAGATTGGAAATCTTTTGCTGTAGGCACCATCAATAGATCGG863540.12944195623109886No Hit
GGGAGATGATCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT854920.12814984508081967Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCAGTCTCATAATCTGAAGGCTGTAGGCACCATCAATAGATCGGAAGAGC838090.12562708050318647No Hit
ACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTC823300.12341010557132695Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TGGAAAAGAAACCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT713800.10699639664376677Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)