FastQCFastQC Report
Wed 27 Sept 2023
SRR4050181.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4050181.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences98948748
Total Bases5 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTACACGGAGTCGAGCACCCGCACTGTAGGCACCATCAATAGATCGGA18503661.8700246717623956No Hit
TAAGCGACGGTCTACAAGAGACCTACCCTGTAGGCACCATCAATAGATCG6805260.6877560492225733No Hit
ATGAGCCCCCCTTAGGACCTGTAGGCACCATCAATAGATCGGAAGAGCAC4590920.46396948852753545No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA3895380.39367653242060224Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGA3820530.38611201022978076No Hit
TTGCCCGAGTTGTAATTTCTGTAGGCACCATCAATAGATCGGAAGAGCAC3176080.3209823331973842No Hit
TCAGATTTAGGCTCTGATACTGTAGGCACCATCAATAGATCGGAAGAGCA3115880.3148983754700969No Hit
CCTGATTCAAGCTCAGGTACTGTAGGCACCATCAATAGATCGGAAGAGCA3108620.3141646622956765No Hit
AAGCACCCAAGGCCTTTCCGCCAAGTGCCTGTAGGCACCATCAATAGATC3104100.3137078601540264No Hit
AAGCGACGGTCTACAAGAGACCTACCCTGTAGGCACCATCAATAGATCGG2380750.24060435812689615No Hit
AACAAAGGCTTAATCTCCTGTAGGCACCATCAATAGATCGGAAGAGCACA2284880.23091550385256013No Hit
ACGACGGAGTTTCACCTGTAGGCACCATCAATAGATCGGAAGAGCACACG2273500.22976541350477725No Hit
GGGAGACAACAAAGGCTTAATCTCAGCCTGTAGGCACCATCAATAGATCG2262740.22867798185784016No Hit
TGGGAGACGCTCAAACCTGTAGGCACCATCAATAGATCGGAAGAGCACAC2200350.2223726974291782No Hit
TGGGAGATGATCCTTCCGCCTGTAGGCACCATCAATAGATCGGAAGAGCA2125440.21480211149311357No Hit
ACGTTCCCTATTAGTGGGTGAACCTGTAGGCACCATCAATAGATCGGAAG1817420.1836728646632295No Hit
AAGGCACGCAAGTAGTCCGCCTAGCCTGTAGGCACCATCAATAGATCGGA1784620.18035801726364442No Hit
AAGGCCGTCTACAACAAGGCACGCCTGTAGGCACCATCAATAGATCGGAA1749990.17685822563414344No Hit
AAAAGGTGCTGGCCTCTTCCAGCCATAAGACCCCCTGTAGGCACCATCAA1738290.17567579531173047No Hit
AAGGCCTTTCCGCCAAGTGCCTGTAGGCACCATCAATAGATCGGAAGAGC1727840.17461969301521632No Hit
AAAAGGCTTAATCTCCTGTAGGCACCATCAATAGATCGGAAGAGCACACG1725350.17436804758762586No Hit
GGGAGATGATCCTTCCGCCTGTAGGCACCATCAATAGATCGGAAGAGCAC1690880.17088442594543996No Hit
AAAAGGCTTAATCTCAGCCTGTAGGCACCATCAATAGATCGGAAGAGCAC1672120.16898849493275045No Hit
AACTCGGGCACCGAAGGTACCCTGTAGGCACCATCAATAGATCGGAAGAG1611030.16281459165102322No Hit
TGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGA1608330.16254172311508175No Hit
AAAAGGTGCTGGCCTCTTCCAGCCCTGTAGGCACCATCAATAGATCGGAA1604300.16213444155958398No Hit
ACGACGGAGTTTCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC1599750.16167460754531227No Hit
TAGCCGCAAAAACCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT1590920.16078222637036296No Hit
TTGATGATTCATAATAACTTTTCGAATCGCACTGTAGGCACCATCAATAG1585090.16019303245757086No Hit
AACAAAGGCTTAATCTCAGCCTGTAGGCACCATCAATAGATCGGAAGAGC1550220.15666898584709735No Hit
TGGGAGAGCAAAGGTGCTGGCCTCTTCCAGCCCTGTAGGCACCATCAATA1458980.14744805058069052No Hit
ACGTTCTAGCATTCAAGGTCCTGTAGGCACCATCAATAGATCGGAAGAGC1453900.1469346534834377No Hit
TGGGAGAACCTACAGCCTGTAGGCACCATCAATAGATCGGAAGAGCACAC1408860.14238280205425136No Hit
TACACGGAGTCGAGCACCCGCACTGTAGGCACCATCAATAGATCGGAAGA1396480.14113164928574942No Hit
AACAAGGCACGCAAGTAGTCCGCCTAGCCTGTAGGCACCATCAATAGATC1377990.13926300512665404No Hit
AAGACCTCTCGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT1370490.13850503697126113No Hit
TACACTGAAAATCAAAATCCTGTAGGCACCATCAATAGATCGGAAGAGCA1280330.12939324911923092No Hit
CAAGCGACGGTCTACAAGAGACCTACCCTGTAGGCACCATCAATAGATCG1272190.12857060101457776No Hit
ACGGAGTCGAGCACCCGCACTGTAGGCACCATCAATAGATCGGAAGAGCA1257730.1271092384109802No Hit
TCACGTTCTAGCATTCAAGGTCCTGTAGGCACCATCAATAGATCGGAAGA1228710.12417640696171316No Hit
GGGAGACAACAAAGGCTTAATCTCCTGTAGGCACCATCAATAGATCGGAA1227330.12403694082112084No Hit
TGTACACGGAGTCGAGCACCCGCACTGTAGGCACCATCAATAGATCGGAA1226110.1239136446678436No Hit
AAATGCTCTATCCCCAGCCTGTAGGCACCATCAATAGATCGGAAGAGCAC1213980.12268775750452143No Hit
ACCACCCGATCCCTAGTCGGCCTGTAGGCACCATCAATAGATCGGAAGAG1165450.11778319822702557No Hit
GGGAGACGCTCAAACCTGTAGGCACCATCAATAGATCGGAAGAGCACACG1154490.1166755540959447No Hit
TTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAATA1144130.11562854741729525No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAA1123600.11355373591993301No Hit
ACCGTTGCTAGCCTGCTATGGTTCAGCGACGCCCTGTAGGCACCATCAAT1100570.11122626837077312No Hit
GACTCCATGGCCAAGTTGGCTGTAGGCACCATCAATAGATCGGAAGAGCA1098200.11098675043366894No Hit
AAGGCACGCAAGTAGTCCGCCTAGCAGAGCCTGTAGGCACCATCAATAGA1070510.10818833200395826No Hit
TTGCCCGAGTTGTAATTTGCTGTAGGCACCATCAATAGATCGGAAGAGCA1067340.10786796413027884No Hit
ACGACGGAGTTTCACAAGATTACCCTGTAGGCACCATCAATAGATCGGAA1053430.10646218585807675No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAAT1041950.10530198926822197No Hit
AAATGCTCTATCCCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACG1037710.10487348460437315No Hit
ATCTCCGGATAAACCCTGTAGGCACCATCAATAGATCGGAAGAGCACACG1023110.10339797326187493No Hit
GGGGAGATGATCCTTCCGCCTGTAGGCACCATCAATAGATCGGAAGAGCA1009610.10203363058216765No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)