FastQCFastQC Report
Mon 3 Jul 2023
SRR4050182.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4050182.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25851321
Total Bases1.2 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA17301756.69279144381055Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ATGTATTTATTAGACTGTAGGCACCATCAATAGATCGGAAGAGCACACGT2199810.850946843296712No Hit
CCTGATTCAAGCTCAGGTACTGTAGGCACCATCAATAGATCGGAAGAGCA1544370.5974046742137471No Hit
TCAGATTTAGGCTCTGATACTGTAGGCACCATCAATAGATCGGAAGAGCA1256450.48602932128690834No Hit
TGGGAGATGATCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT963260.3726153878171255Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGAGATGATCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG794160.3072028698262653Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACGTTCTAGCATTCAAGGTCCTGTAGGCACCATCAATAGATCGGAAGAGC784980.3036517940417822No Hit
GGGAGATGATCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT772390.2987816367295118Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTGCCCGAGTTGTAATTTCTGTAGGCACCATCAATAGATCGGAAGAGCAC741730.2869215078022512No Hit
GGGAGAACCCCCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC671200.2596385693404217No Hit
ATTTATTAGACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGA645030.24951529556265228Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGGGAGACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACT626840.24247890465636168Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GGGAGACAACACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG571830.22119952786938818Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGCGACTGTTAATCGCACTGTAGGCACCATCAATAGATCGGAAGAGCACA559460.21641447259116856No Hit
GGGAGAACCCCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT528240.2043377203045059Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTC464040.17950339945877428Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
AGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGA460750.17823073722228738No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAA413770.16005758467816789No Hit
TGGGAGACAACACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT409460.15839035846562735Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GACTCCATGGCCAAGTTGGCTGTAGGCACCATCAATAGATCGGAAGAGCA399370.15448726972211593No Hit
ATCTGTTGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAAC394020.15241774298497163Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGGGAGATGATCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT374700.14494423708560192Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGAGAACCCCCCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT373690.1445535413838233No Hit
AAATAGAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAA370850.14345495148971305Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAAT353630.1367937831881009No Hit
GGGAGAGCAGCCCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT349030.1350143770215843No Hit
TGGGAGATGATCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC347450.1344031896861286No Hit
ATGCCTTGCTAAGGCATTGCTGTAGGCACCATCAATAGATCGGAAGAGCA334650.12945179861408243No Hit
GGGAGAGCAGCCCCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACG329030.12727782847151214No Hit
TATGTATTTATTAGACTGTAGGCACCATCAATAGATCGGAAGAGCACACG327470.12667437768460652No Hit
ATGTATTTATTAGATCTGTAGGCACCATCAATAGATCGGAAGAGCACACG314470.12164562112705962No Hit
AAATGAAGCGGAGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC283700.1097429411827736No Hit
ATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATAGATCGGAAG269100.10409526074122093No Hit
TATGACTCTTAATCATACTGTAGGCACCATCAATAGATCGGAAGAGCACA268330.10379740362204315No Hit
TGCTAAGGCATTGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC268240.10376258915356781No Hit
TGGGAGACAACCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG267690.10354983406844083Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGGGAGAACCCCCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT263350.10187100303307517No Hit
CAAGACTGTAAATCTTGAGCTGTAGGCACCATCAATAGATCGGAAGAGCA261980.10134104945739522No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)