FastQCFastQC Report
Tue 27 Jun 2023
SRR407274.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR407274.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences193193015
Total Bases9.6 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAAT128201306.635917970429728No Hit
NATTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAAT38100741.9721592936473402No Hit
ATTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAATT26148131.3534718115973292No Hit
CATTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAAT18344380.9495364001643641No Hit
TACTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAAT17001270.8800147355223997No Hit
GATTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAAT16859520.8726775137289514No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA16066780.8316439390937607Illumina Single End Adapter 1 (95% over 22bp)
AGAACCTGAAACCGTGTACGTACAAGCAGTGGGAGCACGCCTGTAGGCAC12565340.6504034320288443No Hit
AATTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAAT11498540.5951840443092624No Hit
ATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCACTGTAGGCACC10464060.5416375949202925No Hit
AGGCACGCTGATATGTAGGTGAAGCGACCTGTAGGCACCATCAATTCGTA7880810.4079241684799008No Hit
AGCACTGTGCAAACACGAAAGTGGACGTATACGGTGCTGTAGGCACCATC6393000.330912585012455No Hit
NTTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAATT5877110.3042092386207648No Hit
ACTTGCCCCGAGATGAGTTCTCCCTGACCCCTGTAGGCACCATCAATTCG5623180.2910653886736019No Hit
ACTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAATT5409110.27998476031858605No Hit
TTTGCCCCGAGATGAGTTCTCCCTGACCCCTGTAGGCACCATCAATTCGT4976170.25757504742083975No Hit
NACTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAAT4512380.233568485899969No Hit
ATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCACTGTAGGCACCATCAAT4247130.21983869344344564No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAT4046790.2094687533087053Illumina Single End Adapter 1 (95% over 22bp)
TAGGCACGCTGATATGTAGGTGAAGCGACCTGTAGGCACCATCAATTCGT3685010.1907424033938287No Hit
ATGGGAAACAGGTTAATATTCCTGTACTTGGTGCTGTAGGCACCATCAAT3449950.178575296834619No Hit
ATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAATTCGTA3199660.165619859496473No Hit
AGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCCTGTAGGCACCATCA3067290.15876816250318368No Hit
GCGAAGGTGGCGGAATTGGTAGACGCGCTAGCCTGTAGGCACCATCAATT2825980.14627754528288717No Hit
NGAACCTGAAACCGTGTACGTACAAGCAGTGGGAGCACGCCTGTAGGCAC2782860.1440455805299172No Hit
TATTCATCTCCGGGGGTAGAGCACTGTTTCGGCTGTAGGCACCATCAATT2700940.13980526159292042No Hit
NTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA2676370.13853347648205605Illumina Single End Adapter 1 (95% over 22bp)
CGGTGGTTCAAGTCCACTCAGGCCCTGTAGGCACCATCAATTCGTATGCC2615840.1354003404315627No Hit
TTGGGAAACAGGTTAATATTCCTGTACTTGGTGCTGTAGGCACCATCAAT2584760.13379158661611032No Hit
NTCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCACTGTAGGCACC2313420.119746565371424No Hit
AGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCCTGTAGGCACCATCA2228050.11532766854950734No Hit
GCGGGAATAGCTCAGTTGGTAGAGCACGACCCTGTAGGCACCATCAATTC2057840.10651730860973414No Hit
TTTGCCCCGAGATGAGTTCTCCCTGACTCCCTGTAGGCACCATCAATTCG2023590.10474447018697854No Hit
AACTTGCCCCGAGATGAGTTCTCCCTGACCCCTGTAGGCACCATCAATTC1979300.10245194423825311No Hit
CACTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAAT1978320.10240121776659472No Hit
GACTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAAT1972910.10212118693835798No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)