FastQCFastQC Report
Tue 27 Jun 2023
SRR407275.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR407275.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences171654193
Total Bases8.5 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAAT135284047.8811963538810845No Hit
ATTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAATT27696841.613525397541556No Hit
CATTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAAT19060131.1103795174988822No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA19028641.108545015267993Illumina Single End Adapter 1 (95% over 22bp)
TACTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAAT17854221.0401272283514797No Hit
GATTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAAT17630341.0270847272574344No Hit
AGAACCTGAAACCGTGTACGTACAAGCAGTGGGAGCACGCCTGTAGGCAC13039490.7596371386045898No Hit
AATTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAAT11991600.6985905669079694No Hit
ATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCACTGTAGGCACC11035930.6429164244184818No Hit
AGGCACGCTGATATGTAGGTGAAGCGACCTGTAGGCACCATCAATTCGTA8346770.4862549439733173No Hit
AGCACTGTGCAAACACGAAAGTGGACGTATACGGTGCTGTAGGCACCATC6711920.39101404298349995No Hit
ACTTGCCCCGAGATGAGTTCTCCCTGACCCCTGTAGGCACCATCAATTCG5920230.3448928276398119No Hit
ACTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAATT5729330.33377163120041003No Hit
TTTGCCCCGAGATGAGTTCTCCCTGACCCCTGTAGGCACCATCAATTCGT5235550.30500565750817404No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAT4424310.2577455244568363Illumina Single End Adapter 1 (95% over 22bp)
ATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCACTGTAGGCACCATCAAT4414290.2571617927212532No Hit
TAGGCACGCTGATATGTAGGTGAAGCGACCTGTAGGCACCATCAATTCGT3884310.2262869279284078No Hit
ATGGGAAACAGGTTAATATTCCTGTACTTGGTGCTGTAGGCACCATCAAT3631640.21156721758611516No Hit
ATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAATTCGTA3402270.19820488742736392No Hit
AGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCCTGTAGGCACCATCA3281170.19115000587256264No Hit
GCGAAGGTGGCGGAATTGGTAGACGCGCTAGCCTGTAGGCACCATCAATT2970150.17303101940539256No Hit
TATTCATCTCCGGGGGTAGAGCACTGTTTCGGCTGTAGGCACCATCAATT2858370.16651908992400788No Hit
CGGTGGTTCAAGTCCACTCAGGCCCTGTAGGCACCATCAATTCGTATGCC2744640.1598935599551594No Hit
TTGGGAAACAGGTTAATATTCCTGTACTTGGTGCTGTAGGCACCATCAAT2720080.15846277637971826No Hit
AGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCCTGTAGGCACCATCA2339050.13626524112929767No Hit
GCGGGAATAGCTCAGTTGGTAGAGCACGACCCTGTAGGCACCATCAATTC2170790.1264629754776803No Hit
TTTGCCCCGAGATGAGTTCTCCCTGACTCCCTGTAGGCACCATCAATTCG2126170.1238635632978683No Hit
AACTTGCCCCGAGATGAGTTCTCCCTGACCCCTGTAGGCACCATCAATTC2069810.12058021792686416No Hit
GACTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAAT2062950.12018057723763263No Hit
CACTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAAT2035130.11855987694981619No Hit
GCGAAGGTGGCGGAATTGGTAGACGCGCCTGTAGGCACCATCAATTCGTA2001240.11658555873435611No Hit
GCGAAGGTGGCGGAATTGGTAGACGCGCTGTAGGCACCATCAATTCGTAT1990240.11594473547173999No Hit
ACTTGCCCCGAGATGAGTTCTCCCTGACTCCCTGTAGGCACCATCAATTC1964470.11444346133741108No Hit
AAGGCACGCTGATATGTAGGTGAAGCGACCTGTAGGCACCATCAATTCGT1904730.11096320845480308No Hit
TTGCCCCGAGATGAGTTCTCCCTGACCCCTGTAGGCACCATCAATTCGTA1802640.10501578601112295No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)