FastQCFastQC Report
Tue 27 Jun 2023
SRR407276.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR407276.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences204841933
Total Bases10.2 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAAT90085384.397799741520697No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA49026752.3933942275383626Illumina Single End Adapter 1 (95% over 22bp)
AGAACCTGAAACCGTGTACGTACAAGCAGTGGGAGCACGCCTGTAGGCAC23612381.1527122232340974No Hit
CATTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAAT12378000.6042708062123198No Hit
TACTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAAT12368670.6038153330646415No Hit
ATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCACTGTAGGCACC12018200.5867060432396916No Hit
GATTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAAT11172110.5454015121015285No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAT9363670.45711685409647057Illumina Single End Adapter 1 (95% over 22bp)
AATTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAAT8294330.4049136755607554No Hit
CGGTGGTTCAAGTCCACTCAGGCCCTGTAGGCACCATCAATTCGTATGCC6212660.3032904400487179No Hit
AGGCACGCTGATATGTAGGTGAAGCGACCTGTAGGCACCATCAATTCGTA6039040.29481463641528904No Hit
TTTGCCCCGAGATGAGTTCTCCCTGACCCCTGTAGGCACCATCAATTCGT5865730.2863539664019866No Hit
ATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCACTGTAGGCACCATCAAT5607580.27375156628696723No Hit
ACTTGCCCCGAGATGAGTTCTCCCTGACCCCTGTAGGCACCATCAATTCG4900480.23923226695971472No Hit
AGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCCTGTAGGCACCATCA4610350.22506866306519377No Hit
CCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA4402980.21494524756315397Illumina Single End Adapter 1 (95% over 22bp)
ATTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAATT4326840.21122823518756775No Hit
TAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTACTGTAGGC4079850.19917064539710239No Hit
ATGTACACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAATTCGTA3753190.1832237152341264No Hit
AGCACTGTGCAAACACGAAAGTGGACGTATACGGTGCTGTAGGCACCATC3748310.18298548276245763No Hit
ATGGGAAACAGGTTAATATTCCTGTACTTGGTGCTGTAGGCACCATCAAT3743480.18274969119726087No Hit
TAGGCACGCTGATATGTAGGTGAAGCGACCTGTAGGCACCATCAATTCGT3702530.18075058879667963No Hit
AGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCCTGTAGGCACCATCA3680380.17966926722957646No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAG3592110.17536009094387917Illumina Single End Adapter 1 (95% over 22bp)
GCGGGAATAGCTCAGTTGGTAGAGCACGACCCTGTAGGCACCATCAATTC2916820.14239369631412335No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAATA2851480.13920391973649263Illumina Single End Adapter 1 (95% over 22bp)
TATTCATCTCCGGGGGTAGAGCACTGTTTCGGCTGTAGGCACCATCAATT2838810.13858539403648373No Hit
AGAACCTGAAACCGTGTACGTACAAGCAGTGGGAGCATGCCTGTAGGCAC2810060.13718187281507443No Hit
TAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGCA2745620.13403603255394003No Hit
GCGAAGGTGGCGGAATTGGTAGACGCGCTGTAGGCACCATCAATTCGTAT2689880.1313149100189364No Hit
TTTGCCCCGAGATGAGTTCTCCCTGACTCCCTGTAGGCACCATCAATTCG2658920.12980350073146402No Hit
ACGCAGGAGGTCTGCGGTTCGATCCCGCATAGCTCCCTGTAGGCACCATC2647750.1292582022256156No Hit
ACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACCTGTAGGCACCATCA2609170.12737479879180794No Hit
TTGGGAAACAGGTTAATATTCCTGTACTTGGTGCTGTAGGCACCATCAAT2604840.12716341629133132No Hit
TTATCTGCCGTGGGCGCTGGAGAACTGAGGGGGGCTGCTCCCTGTAGGCA2555920.12477523339911072No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAC2433220.11878524891678306Illumina Single End Adapter 1 (95% over 22bp)
GCGAAGGTGGCGGAATTGGTAGACGCGCTAGCCTGTAGGCACCATCAATT2304170.11248526931250938No Hit
ACCGCCAGCTAAGGTCCCAAAGTCATGGTTAAGTGGCTGTAGGCACCATC2124000.10368970693124635No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)