FastQCFastQC Report
Mon 26 Jun 2023
SRR407278.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR407278.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18230224
Total Bases911.5 Mbp
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCAGTAGGTTGGGGGTTCAAGTCCTCTTGCCGGCACCACTGTAGGCACC12615356.920019194498103No Hit
ATCAGAGGGTCGAAGGTTCGAGTCCTTCATGGCTCACCACTGTAGGCACC3088491.6941591063280408No Hit
GCCGGTGTAGCTCAATTGGTAGAGCAACTGCTGTAGGCACCATCAATTCG2338731.2828860468198306No Hit
GCCGGTGTAGCTCAATTGGTAGAGCAACTGACTGTAGGCACCATCAATTC2335341.2810264975350825No Hit
AAGAGTCTTGGAGGTAGAGCACTGATTGGACTAGGGGCCCCCTGTAGGCA1869981.0257581036853962No Hit
ACGGGGGCCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGCTGTAGGCA1382850.758548002482032No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA1200470.6585053480418013Illumina Single End Adapter 1 (95% over 22bp)
TAAGCGAAGGTGCGAATTGAAGCCCCAGTAAACGGCGGCCGTCTGTAGGC1172760.6433053153927236No Hit
ACGGGGGCCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGACTGTAGGC1143550.6272824733256158No Hit
TATCAGTAGGTTGGGGGTTCAAGTCCTCTTGCCGGCACCACTGTAGGCAC957630.5252979886588338No Hit
TAGCCGGTGTAGCTCAATTGGTAGAGCAACTGCTGTAGGCACCATCAATT669990.3675160546573646No Hit
TGCCGGTGTAGCTCAATTGGTAGAGCAACTGCTGTAGGCACCATCAATTC623720.34213512680919334No Hit
AGTAGGTTGGGGGTTCAAGTCCTCTTGCCGGCACCACTGTAGGCACCATC600360.32932124147240316No Hit
TGCCGGTGTAGCTCAATTGGTAGAGCAACTGACTGTAGGCACCATCAATT572390.31397858852420024No Hit
TTAAGAGTCTTGGAGGTAGAGCACTGATTGGACTAGGGGCCCCCTGTAGG567700.31140593774382586No Hit
AGCGGACAGATGGGAGCTTGCTCCCTGATGCTGTAGGCACCATCAATTCG563340.3090143050354181No Hit
ACTACCGTGCCGGTTCAAGTCCGGCCCTCGGCACCACTGTAGGCACCATC540100.2962662444520704No Hit
TTAGCGAAGTTCCTGATTCCACACTGCCACTGTAGGCACCATCAATTCGT536380.29422567709535546No Hit
TTACCGGGGCTAAACGTATCACCGAAGCTGCGGACTGTTCCTGTAGGCAC528310.28979896242635306No Hit
AGCCGGTGTAGCTCAATTGGTAGAGCAACTGCTGTAGGCACCATCAATTC496140.2721524431076656No Hit
AGCGAAGGTGCGAATTGAAGCCCCAGTAAACGGCGGCCGTCTGTAGGCAC452180.24803864176326085No Hit
AAGAGTCTTGGAGGTAGAGCACTGATTGGACTAGGGGCCCCTGTAGGCAC374440.2053951723248162No Hit
TTAGCGAAGGTGCGAATTGAAGCCCCAGTAAACGGCGGCCGTCTGTAGGC367100.20136889157258844No Hit
TAGCGAAGGTGCGAATTGAAGCCCCAGTAAACGGCGGCCGTCTGTAGGCA365150.20029923932914923No Hit
GCCGGTGTAGCTCAATTGGTAGAGCAACTGACCTGTAGGCACCATCAATT348920.19139644142606255No Hit
AAGCGAAGGTGCGAATTGAAGCCCCAGTAAACGGCGGCCGTCTGTAGGCA341730.18745244161563784No Hit
ACGACGTGGGGGTTCGAGTCCCTTCACCCGCACCACTGTAGGCACCATCA339980.18649249729460263No Hit
TAGCCGGTGTAGCTCAATTGGTAGAGCAACTGACTGTAGGCACCATCAAT326580.17914206649353295No Hit
TAAGCGAAGGTGCGAATTGAAGCCCCAGTAAACGGCGGCCGTACTGTAGG318860.17490734068873756No Hit
TATCAGAGGGTCGAAGGTTCGAGTCCTTCATGGCTCACCACTGTAGGCAC296610.16270233432128972No Hit
AGAGGGTCGAAGGTTCGAGTCCTTCATGGCTCACCACTGTAGGCACCATC287770.15785324415103183No Hit
AGGAGCCAGCCGCCGAAGGTGGGACAGATGATTGGGGTGAACTGTAGGCA272500.1494770442754845No Hit
TAAGCGAAGGTGCGAATTGAAGCCCCAGTAAACGGCGGCCGCTGTAGGCA265640.14571406253702643No Hit
ACCTGGGCTACACACGTGCTACAATGGACAGAACAAAGGGCCTGTAGGCA253750.13919192655010712No Hit
TAGCGGCGCGAGGGTTCAAATCCCTCCTTCTCCGCCACTGTAGGCACCAT248710.13642728690552566No Hit
AGCGAAGTTCCTGATTCCACACTGCCACTGTAGGCACCATCAATTCGTAT248450.13628466660640046No Hit
AGGAGCCAGCCGCCGAAGGTGGGACAGATGATTGGGGTGAAGCTGTAGGC247120.13555510892241368No Hit
AGCCGGTGTAGCTCAATTGGTAGAGCAACTGACTGTAGGCACCATCAATT244030.1338601215212715No Hit
ACCGGGATGGACGCACCGCTGGTGTACCAGTCTGTAGGCACCATCAATTC244030.1338601215212715No Hit
AATCAGTAGGTTGGGGGTTCAAGTCCTCTTGCCGGCACCACTGTAGGCAC237240.13013553755565482No Hit
TAAGCGGCGCGAGGGTTCAAATCCCTCCTTCTCCGCCACTGTAGGCACCA236640.12980641378844274No Hit
TAACCCGTGGAGGTTCAAGTCCTCTCGGCCGCACCACTGTAGGCACCATC233290.12796880608817535No Hit
TAAGAGTCTTGGAGGTAGAGCACTGATTGGACTAGGGGCCCCCTGTAGGC233150.1278920105424925No Hit
TAAGCGAAGGTGCGAATTGAAGCCCCAGTAAACGGCGGCCGTCTGTAGGG224960.12339947112004768No Hit
ACCGGGGCTAAACGTATCACCGAAGCTGCGGACTGTTCCTGTAGGCACCA222230.12190195797923273No Hit
ACAACAGGTTGATATTCCTGTACCACCTCCTCACCCTGTAGGCACCATCA219030.12014663122076832No Hit
AGGAGCCAGCCGCCGAAGGTGGGACAGATGATTGGGGTGACTGTAGGCAC213020.11684990815252737No Hit
ACTCCGTCTCAAGGGTTCGAATCCCTTGCTCTCCGCCACTGTAGGCACCA200330.10988894047599196No Hit
TAGCGAAGTTCCTGATTCCACACTGCCACTGTAGGCACCATCAATTCGTA199140.109236178337688No Hit
ATGACGTGGGGGTTCAAGTCCCTTCACCCGCACCACTGTAGGCACCATCA194720.10681163325255905No Hit
TTAACCTGGAGATAGCTGGTTCTCTCCGAAATAGCCTGTAGGCACCATCA192530.10561033150223496No Hit
ACCTGGGCTACACACGTGCTACAATGGACAGAACAAAGGGCTGTAGGCAC190510.10450228148595432No Hit
ACCCGTGGAGGTTCAAGTCCTCTCGGCCGCACCACTGTAGGCACCATCAA189900.10416767232262203No Hit
TTGACGTGGGGGTTCAAGTCCCTTCACCCGCACCACTGTAGGCACCATCA185460.10173215644525267No Hit
AAGCGGCGCGAGGGTTCAAATCCCTCCTTCTCCGCCACTGTAGGCACCAT182660.10019624553159633No Hit
TTGGAGCTGACAGATACTAATCGATCGAGGACCTGTAGGCACCATCAATT182490.10010299379755289No Hit
TTACCGGGGCTAAACGTATCACCGAAGCTGCGGACTGTCTGTAGGCACCA182370.10003716904411047No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)