FastQCFastQC Report
Mon 26 Jun 2023
SRR407279.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR407279.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15991160
Total Bases799.5 Mbp
Sequences flagged as poor quality0
Sequence length50
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCAGTAGGTTGGGGGTTCAAGTCCTCTTGCCGGCACCACTGTAGGCACC8976265.613263828265117No Hit
ATCAGAGGGTCGAAGGTTCGAGTCCTTCATGGCTCACCACTGTAGGCACC3001711.8771058509826677No Hit
GCCGGTGTAGCTCAATTGGTAGAGCAACTGCTGTAGGCACCATCAATTCG2556601.5987583139684676No Hit
AAGAGTCTTGGAGGTAGAGCACTGATTGGACTAGGGGCCCCCTGTAGGCA1756651.0985131785311384No Hit
GCCGGTGTAGCTCAATTGGTAGAGCAACTGACTGTAGGCACCATCAATTC1647351.0301629150105434No Hit
TAAGCGAAGGTGCGAATTGAAGCCCCAGTAAACGGCGGCCGTCTGTAGGC1409680.8815370492196939No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA1270100.7942513238564307Illumina Single End Adapter 1 (95% over 22bp)
ACGGGGGCCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGCTGTAGGCA853490.5337261336888631No Hit
TAGCCGGTGTAGCTCAATTGGTAGAGCAACTGCTGTAGGCACCATCAATT723890.4526813564494383No Hit
TATCAGTAGGTTGGGGGTTCAAGTCCTCTTGCCGGCACCACTGTAGGCAC711320.44482076347181815No Hit
TATTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAAT682360.42671075769362576No Hit
TGCCGGTGTAGCTCAATTGGTAGAGCAACTGCTGTAGGCACCATCAATTC681650.42626676238621836No Hit
AAGAGTCTTGGAGGTAGAGCACTGATTGGACTAGGGGCCCCTGTAGGCAC609040.38086042538502524No Hit
AGCGAAGGTGCGAATTGAAGCCCCAGTAAACGGCGGCCGTCTGTAGGCAC559450.3498495418718842No Hit
AGCCGGTGTAGCTCAATTGGTAGAGCAACTGCTGTAGGCACCATCAATTC521560.3261552007484135No Hit
ACGGGGGCCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGACTGTAGGC472170.2952693863359506No Hit
TTAGCGAAGGTGCGAATTGAAGCCCCAGTAAACGGCGGCCGTCTGTAGGC440210.2752833440475863No Hit
TAGCGAAGGTGCGAATTGAAGCCCCAGTAAACGGCGGCCGTCTGTAGGCA439260.2746892658193652No Hit
TGCCGGTGTAGCTCAATTGGTAGAGCAACTGACTGTAGGCACCATCAATT415220.2596559599178546No Hit
AAGCGAAGGTGCGAATTGAAGCCCCAGTAAACGGCGGCCGTCTGTAGGCA388150.24272785714107045No Hit
AGTAGGTTGGGGGTTCAAGTCCTCTTGCCGGCACCACTGTAGGCACCATC383790.2400013507462873No Hit
TAAGCGAAGGTGCGAATTGAAGCCCCAGTAAACGGCGGCCGCTGTAGGCA353560.22109715617878878No Hit
TTAAGAGTCTTGGAGGTAGAGCACTGATTGGACTAGGGGCCCCCTGTAGG338630.21176074781316676No Hit
AGCGGACAGATGGGAGCTTGCTCCCTGATGCTGTAGGCACCATCAATTCG335980.2101035822291816No Hit
TTAGCGAAGTTCCTGATTCCACACTGCCACTGTAGGCACCATCAATTCGT325530.20356872171874962No Hit
ACTACCGTGCCGGTTCAAGTCCGGCCCTCGGCACCACTGTAGGCACCATC325030.2032560489670543No Hit
AGAGGGTCGAAGGTTCGAGTCCTTCATGGCTCACCACTGTAGGCACCATC323060.20202411832537476No Hit
AAGAGTCTTGGAGGTAGAGCACTGATTGGCTGTAGGCACCATCAATTCGT320570.20046700802193212No Hit
TAAGCGAAGGTGCGAATTGAAGCCCCAGTAAACGGCGGCCGTACTGTAGG320070.2001543352702368No Hit
TATCAGAGGGTCGAAGGTTCGAGTCCTTCATGGCTCACCACTGTAGGCAC301410.18848538817696778No Hit
AAGAGTCTTGGAGGTAGAGCACTGATTGGACTCTGTAGGCACCATCAATT282820.17686021526893608No Hit
ACCTGGGCTACACACGTGCTACAATGGACAGAACAAAGGGCTGTAGGCAC281920.17629740431588453No Hit
ACCGGGATGGACGCACCGCTGGTGTACCAGTCTGTAGGCACCATCAATTC270670.16926226740274No Hit
TTGGAGCTGACAGATACTAATCGATCGAGGACCTGTAGGCACCATCAATT267810.16747377926304283No Hit
TTACCGGGGCTAAACGTATCACCGAAGCTGCGGACTGTTCCTGTAGGCAC253210.15834373491353973No Hit
TAAGCGAAGGTGCGAATTGAAGCCCCAGTAAACGGCGGCCGTCTGTAGGG253160.1583124676383702No Hit
ACGATGAGTGCTAAGTGTTAGGGGGCTGTAGGCACCATCAATTCGTATGC235690.14738768169413602No Hit
AAGAGTCTTGGAGGTAGAGCACTGATTGGACTGTAGGCACCATCAATTCG228510.14289770097979132No Hit
ACGACGTGGGGGTTCGAGTCCCTTCACCCGCACCACTGTAGGCACCATCA222910.13939576616080385No Hit
AGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCCTGTAGGCACCATCA217540.1360376608075962No Hit
ACGGCGGTAACACGGGTTCGAATCCCGTACGGGTCCTGTAGGCACCATCA216530.13540606184917167No Hit
TAGCCGGTGTAGCTCAATTGGTAGAGCAACTGACTGTAGGCACCATCAAT213650.13360506679940667No Hit
ACCTGGGCTACACACGTGCTACAATGGACAGAACAAAGGGCCTGTAGGCA207980.13005935779518185No Hit
AGAGCACTGATTGGACTAGGGGCCCCCTGTAGGCACCATCAATTCGTATG205180.12830839038568811No Hit
AGGAAGAGGTCAGCGGTTCGAGCCCGCTTGGACTGTAGGCACCATCAATT198210.12394973222705544No Hit
AGGAGCCAGCCGCCGAAGGTGGGACAGATGATTGGGGTGACTGTAGGCAC192730.12052283886847483No Hit
AAAGCGAAGGTGCGAATTGAAGCCCCAGTAAACGGCGGCCGTCTGTAGGC191400.11969112934896531No Hit
TAAGGTGCGAATTGAAGCCCCAGTAAACGGCGGCCGTCTGTAGGCACCAT189880.11874060418381156No Hit
TGAGCCATTAGCTCAGTTGGTAGAGCATCTGCTGTAGGCACCATCAATTC183530.11476966023728111No Hit
AGGAGCCAGCCGCCGAAGGTGGGACAGATGATTGGGGTGAACTGTAGGCA181100.11325007066404187No Hit
TTAAGAGTCTTGGAGGTAGAGCACTGATTGGACTAGGGGCCCCTGTAGGC180230.11270602007609205No Hit
AATCAGTAGGTTGGGGGTTCAAGTCCTCTTGCCGGCACCACTGTAGGCAC176190.11017962424239391No Hit
ATGTACACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAATTCGTA175430.10970436165981705No Hit
ACCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA173930.10876634340473111Illumina Single End Adapter 1 (95% over 22bp)
ACAACAGGTTGATATTCCTGTACCACCTCCTCACCCTGTAGGCACCATCA168020.10507055147969252No Hit
TTGCTGAAGCAACAAATGCCCTGCTTCCAGGAAAAGCCTCTAAGCCTGTA166140.10389490193331814No Hit
TAAGCGAAGGTGCGAATTGAAGCCCCAGTAAACGGCGGCCGGCTGTAGGC165460.10346966699101252No Hit
GCCGGTGTAGCTCAATTGGTAGAGCAACTGACCTGTAGGCACCATCAATT165420.10344465317087691No Hit
AGCGAAGGTGCGAATTGAAGCCCCAGTAAACGGCGGCCGCTGTAGGCACC165010.10318826151448676No Hit
CCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA163510.10225024325940081Illumina Single End Adapter 1 (95% over 22bp)
AAGGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATTCGTATG163200.10205638615334972No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)