FastQCFastQC Report
Mon 26 Jun 2023
SRR407280.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR407280.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35407892
Total Bases1.7 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA7769552.1942989432977256Illumina Single End Adapter 1 (95% over 22bp)
TAAGCGAAGGTGCGAATTGAAGCCCCAGTAAACGGCGGCCGTCTGTAGGC5131491.4492503535652446No Hit
AGCGAAGGTGCGAATTGAAGCCCCAGTAAACGGCGGCCGTCTGTAGGCAC3087790.8720626463727352No Hit
AAGCGAAGGTGCGAATTGAAGCCCCAGTAAACGGCGGCCGTCTGTAGGCA2677910.7563031428134722No Hit
TAGCGAAGGTGCGAATTGAAGCCCCAGTAAACGGCGGCCGTCTGTAGGCA2476140.699318671667887No Hit
ACCGGGATGGACGCACCGCTGGTGTACCAGTCTGTAGGCACCATCAATTC2069370.5844375033678932No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAT1666020.47052222143018285Illumina Single End Adapter 1 (95% over 22bp)
TTAGCGAAGGTGCGAATTGAAGCCCCAGTAAACGGCGGCCGTCTGTAGGC1623620.45854748992117356No Hit
ATGTACACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAATTCGTA1604970.45328030259468705No Hit
ACGGGGGCCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGCTGTAGGCA1379610.3896334749326506No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAC1377050.38891047227550285Illumina Single End Adapter 1 (95% over 22bp)
TTGGAGCTGACAGATACTAATCGATCGAGGACCTGTAGGCACCATCAATT1240370.35030890853372465No Hit
TTTGGGCACTCTAAGGTGACTGCCGGTGCTGTAGGCACCATCAATTCGTA1224590.34585227496739995No Hit
ACAACAGGTTGATATTCCTGTACCACCTCCTCACCCTGTAGGCACCATCA1220370.34466045027475795No Hit
CCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA1202840.33970957661077367Illumina Single End Adapter 1 (95% over 22bp)
AAGAGTCTTGGAGGTAGAGCACTGATTGGACTAGGGGCCCCCTGTAGGCA1197330.3381534263604284No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAACA1148650.3244050789581035Illumina Single End Adapter 1 (95% over 22bp)
TAAGCGAAGGTGCGAATTGAAGCCCCAGTAAACGGCGGCCGCTGTAGGCA1138410.32151306832951254No Hit
ACTACCGTGCCGGTTCAAGTCCGGCCCTCGGCACCACTGTAGGCACCATC1126990.31828779866364254No Hit
ACCGGGATGGACGCACCGCTGGTGTACCAGCTGTAGGCACCATCAATTCG1053320.2974817026667388No Hit
ACCTGGGCTACACACGTGCTACAATGGACAGAACAAAGGGCTGTAGGCAC1042440.2944089413738609No Hit
ACCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA972180.274565907510111Illumina Single End Adapter 1 (95% over 22bp)
TTGCGTTGGTTCGAATCCAGCTAGCCCAGCCACTGTAGGCACCATCAATT951480.2687197532120805No Hit
AGCGGACAGATGGGAGCTTGCTCCCTGATGCTGTAGGCACCATCAATTCG949340.2681153681783711No Hit
ACCCGTGGAGGTTCAAGTCCTCTCGGCCGCACCACTGTAGGCACCATCAA932910.26347515971862995No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAG919060.25956360237429554Illumina Single End Adapter 1 (95% over 22bp)
ACGACGTGGGGGTTCGAGTCCCTTCACCCGCACCACTGTAGGCACCATCA905300.25567746309212647No Hit
TTAGCGAAGTTCCTGATTCCACACTGCCACTGTAGGCACCATCAATTCGT871580.24615416246750865No Hit
AGGTGAGAGGTGCCCGTACCGCAAACCCTGTAGGCACCATCAATTCGTAT865400.24440878886548795No Hit
TAACCCGTGGAGGTTCAAGTCCTCTCGGCCGCACCACTGTAGGCACCATC815700.23037237009195577No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAATA809850.228720196051208Illumina Single End Adapter 1 (95% over 22bp)
ATCAGAGGGTCGAAGGTTCGAGTCCTTCATGGCTCACCACTGTAGGCACC808710.2283982339304469No Hit
TAGCGGCGCGAGGGTTCAAATCCCTCCTTCTCCGCCACTGTAGGCACCAT804780.22728831188256No Hit
ACAACAGGTTGATATTCCTGTACCACCTCCCTGTAGGCACCATCAATTCG789480.22296724131445045No Hit
AGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCCTGTAGGCACCATCA749680.21172680937910676No Hit
TAAGGTGCGAATTGAAGCCCCAGTAAACGGCGGCCGTCTGTAGGCACCAT747330.2110631155336782No Hit
AAAGCGAAGGTGCGAATTGAAGCCCCAGTAAACGGCGGCCGTCTGTAGGC689000.19458938702140188No Hit
AGCGAAGGTGCGAATTGAAGCCCCAGTAAACGGCGGCCGCTGTAGGCACC678110.19151380149939454No Hit
AAGCGGCGCGAGGGTTCAAATCCCTCCTTCTCCGCCACTGTAGGCACCAT665980.18808801156533125No Hit
AAGGCTGAGCTGTGATGGCGAGCGCTGTAGGCACCATCAATTCGTATGCC661890.18693290185137257No Hit
AAGAGTCTTGGAGGTAGAGCACTGATTGGACTAGGGGCCCCTGTAGGCAC629300.17772873911838638No Hit
TAAGCGGCGCGAGGGTTCAAATCCCTCCTTCTCCGCCACTGTAGGCACCA627020.17708481487686417No Hit
TGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA598820.16912048873172117Illumina Single End Adapter 1 (95% over 22bp)
AGCGAAGTTCCTGATTCCACACTGCCACTGTAGGCACCATCAATTCGTAT598800.1691148402734622No Hit
AAAAGGGCTCGCAGGCGGTTTCTTAAGTCTGACTGTAGGCACCATCAATT589630.166525022161726No Hit
AAGCGAAGGTGCGAATTGAAGCCCCAGTAAACGGCGGCCGCTGTAGGCAC585870.16546311200904024No Hit
ACGGCGGTAACACGGGTTCGAATCCCGTACGGGTCCTGTAGGCACCATCA585840.1654546393216518No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGA584070.16495475076573327Illumina Single End Adapter 1 (95% over 22bp)
ATGATCAGGTTGATAGGTCTGAGGTGGACTGTAGGCACCATCAATTCGTA580820.16403687629865116No Hit
TTCGCTGGGTAGCTATGTGCGGACGGGCTGTAGGCACCATCAATTCGTAT563540.15915660836290393No Hit
ATGACGTGGGGGTTCAAGTCCCTTCACCCGCACCACTGTAGGCACCATCA558160.15763717309124192No Hit
CAAGCGAAGGTGCGAATTGAAGCCCCAGTAAACGGCGGCCGTCTGTAGGC540880.1527569051554947No Hit
TAAGCGAAGGTGCGAATTGAAGCCCCAGTAAACGGCGGCCGGCTGTAGGC539740.1524349430347336No Hit
ACGATGAGTGCTAAGTGTTAGGGGGCTGTAGGCACCATCAATTCGTATGC524310.1480771574879408No Hit
AGCGAAGGTGCGAATTGAAGCCCCAGTAAACGGCGGCCGTACTGTAGGCA504900.14259532874761366No Hit
TAAGCGAAGGTGCGAATTGAAGCCCCAGTAAACGGCGGCCGTCTGTAGGG504080.14236374195899604No Hit
ACTCCGTCTCAAGGGTTCGAATCCCTTGCTCTCCGCCACTGTAGGCACCA500790.14143457057539602No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAATC499520.14107589347595165Illumina Single End Adapter 1 (95% over 22bp)
TAGCGAAGGTGCGAATTGAAGCCCCAGTAAACGGCGGCCGCTGTAGGCAC497450.14049127804614858No Hit
TAAGCGAAGGTGCGAATTGAAGCCCCAGTAAACGGCGGCCGTACTGTAGG496820.14031335161099112No Hit
AGCGGCGCGAGGGTTCAAATCCCTCCTTCTCCGCCACTGTAGGCACCATC475490.13428927087780318No Hit
AGAACTCTGAACCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTG464920.1313040606879393Illumina Single End Adapter 1 (95% over 22bp)
AGGTGCGAATTGAAGCCCCAGTAAACGGCGGCCGTCTGTAGGCACCATCA461470.13032970163826754No Hit
TTGACGTGGGGGTTCAAGTCCCTTCACCCGCACCACTGTAGGCACCATCA457110.1290983377378128No Hit
CAGCGAAGGTGCGAATTGAAGCCCCAGTAAACGGCGGCCGTCTGTAGGCA455270.12857867957798788No Hit
AAGAGTCTTGGAGGTAGAGCACTGATTGGACTCTGTAGGCACCATCAATT450230.12715526809672825No Hit
TTGCCTCTTGGGGTTGTAGGACACTCCTGTAGGCACCATCAATTCGTATG448180.12657630112518417No Hit
GGGGCCTTAGCTCAGCTGGGAGAGCGCCTGCCTGTAGGCACCATCAATTC430030.12145032525517194No Hit
ATCAGTAGGTTGGGGGTTCAAGTCCTCTTGCCGGCACCACTGTAGGCACC427890.1208459402214625No Hit
TGGGGACGTTACGGATTCGACAGGGATGGCTGTAGGCACCATCAATTCGT413270.11671691723415785No Hit
TTCCGTCTCAAGGGTTCGAATCCCTTGCTCTCCGCCACTGTAGGCACCAT413200.11669714763025148No Hit
TCGCGGGCGCAGGAAATTTGAGAGGAGCTGTCCTCTGTAGGCACCATCAA402250.11360461673346721No Hit
ATCAGAGGGTCGAAGGTTCGAGTCCTTCATGGCCTGTAGGCACCATCAAT398620.11257942155946477No Hit
GCCGGTGTAGCTCAATTGGTAGAGCAACTGCTGTAGGCACCATCAATTCG395920.11181687969450427No Hit
TAGCGAAGGTGCGAATTGAAGCCCCAGTAAACGGCGGCCGTACTGTAGGC391310.11051491006581246No Hit
ACCGCCAGCTAAGGTCCCAAAGTATACGTTAAGTGGCTGTAGGCACCATC386130.10905195937674007No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAATT369490.1043524421052798Illumina Single End Adapter 1 (95% over 22bp)
AGCGAAGGTGCGAATTGAAGCCCCAGTAAACGGCGGCCGTCTGTAGGGAC368390.10404177690103664No Hit
GAAGCGAAGGTGCGAATTGAAGCCCCAGTAAACGGCGGCCGTCTGTAGGC368140.10397117117279955No Hit
ACGCAGGAGGTCGCGGGTTCGAGTCCCGTCCGGACCGCCCTGTAGGCACC364980.10307871476788281No Hit
TTACCGTGCCGGTTCAAGTCCGGCCCTCGGCACCACTGTAGGCACCATCA363720.10272286189756791No Hit
TTGGAGCTGACAGATACTAATCGATCGAGGCTGTAGGCACCATCAATTCG359150.10143218918539404No Hit
ACCGTGCCGGTTCAAGTCCGGCCCTCGGCACCACTGTAGGCACCATCAAT357840.10106221516943172No Hit
TAAGCGAAGGTGCGAATTGAAGCCCCAGTAAACGGCGGCCGTCTGGAGGC357280.10090405833818065No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)