FastQCFastQC Report
Sun 2 Jul 2023
SRR407281.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR407281.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences176894215
Total Bases8.8 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAAGCGAAGGTGCGAATTGAAGCCCCAGTAAACGGCGGCCGTCTGTAGGC31955091.806451952088993No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA21183891.1975456630958792Illumina Single End Adapter 1 (95% over 22bp)
AGCGAAGGTGCGAATTGAAGCCCCAGTAAACGGCGGCCGTCTGTAGGCAC19097351.0795915513687093No Hit
TAGCGAAGGTGCGAATTGAAGCCCCAGTAAACGGCGGCCGTCTGTAGGCA16109270.9106725169050892No Hit
AAGCGAAGGTGCGAATTGAAGCCCCAGTAAACGGCGGCCGTCTGTAGGCA15249360.8620609780822963No Hit
TTAGCGAAGGTGCGAATTGAAGCCCCAGTAAACGGCGGCCGTCTGTAGGC11188360.632488744756294No Hit
TAAGCGAAGGTGCGAATTGAAGCCCCAGTAAACGGCGGCCGCTGTAGGCA7255150.41014060295866656No Hit
ACCGGGATGGACGCACCGCTGGTGTACCAGTCTGTAGGCACCATCAATTC7184240.40613199250184634No Hit
ATGTACACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAATTCGTA5805920.32821423809704575No Hit
ACGGGGGCCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGCTGTAGGCA5581380.3155207760751249No Hit
TAAGGTGCGAATTGAAGCCCCAGTAAACGGCGGCCGTCTGTAGGCACCAT4765150.2693785096363948No Hit
TTTGGGCACTCTAAGGTGACTGCCGGTGCTGTAGGCACCATCAATTCGTA4672990.26416861625463556No Hit
ACTACCGTGCCGGTTCAAGTCCGGCCCTCGGCACCACTGTAGGCACCATC4645340.26260553517818547No Hit
AAGAGTCTTGGAGGTAGAGCACTGATTGGACTAGGGGCCCCCTGTAGGCA4640230.2623166619665883No Hit
AAAGCGAAGGTGCGAATTGAAGCCCCAGTAAACGGCGGCCGTCTGTAGGC4174280.23597606060774798No Hit
AGCGAAGGTGCGAATTGAAGCCCCAGTAAACGGCGGCCGCTGTAGGCACC4143610.23424225602855356No Hit
ACAACAGGTTGATATTCCTGTACCACCTCCTCACCCTGTAGGCACCATCA4118000.2327944981128976No Hit
TTGGAGCTGACAGATACTAATCGATCGAGGACCTGTAGGCACCATCAATT4028020.2277078422264968No Hit
ACCGGGATGGACGCACCGCTGGTGTACCAGCTGTAGGCACCATCAATTCG3769970.21312002769564847No Hit
ACCTGGGCTACACACGTGCTACAATGGACAGAACAAAGGGCTGTAGGCAC3583790.20259509334434708No Hit
AAGCGAAGGTGCGAATTGAAGCCCCAGTAAACGGCGGCCGCTGTAGGCAC3539760.20010603512387332No Hit
AGCGAAGGTGCGAATTGAAGCCCCAGTAAACGGCGGCCGTACTGTAGGCA3532000.19966735486516618No Hit
AGGTGAGAGGTGCCCGTACCGCAAACCCTGTAGGCACCATCAATTCGTAT3476050.19650444758750307No Hit
TAGCGAAGGTGCGAATTGAAGCCCCAGTAAACGGCGGCCGCTGTAGGCAC3471450.1962444051661045No Hit
ACGACGTGGGGGTTCGAGTCCCTTCACCCGCACCACTGTAGGCACCATCA3448780.19496284827629892No Hit
ACCCGTGGAGGTTCAAGTCCTCTCGGCCGCACCACTGTAGGCACCATCAA3412220.19289607633579198No Hit
TTGCGTTGGTTCGAATCCAGCTAGCCCAGCCACTGTAGGCACCATCAATT3388830.19157381715394142No Hit
TAACCCGTGGAGGTTCAAGTCCTCTCGGCCGCACCACTGTAGGCACCATC3221820.18213258132833796No Hit
AGCGGACAGATGGGAGCTTGCTCCCTGATGCTGTAGGCACCATCAATTCG3205160.18119077551518573No Hit
TAAGCGAAGGTGCGAATTGAAGCCCCAGTAAACGGCGGCCGTACTGTAGG3135430.17724887159255037No Hit
CAAGCGAAGGTGCGAATTGAAGCCCCAGTAAACGGCGGCCGTCTGTAGGC3125240.17667282109819138No Hit
TAGCGAAGGTGCGAATTGAAGCCCCAGTAAACGGCGGCCGTACTGTAGGC2923580.16527278746792257No Hit
AGGTGCGAATTGAAGCCCCAGTAAACGGCGGCCGTCTGTAGGCACCATCA2908920.16444404357711756No Hit
AGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCCTGTAGGCACCATCA2870740.1622856914795094No Hit
TAGCGGCGCGAGGGTTCAAATCCCTCCTTCTCCGCCACTGTAGGCACCAT2840300.16056488902138488No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAT2815000.15913465570369273Illumina Single End Adapter 1 (95% over 22bp)
CAGCGAAGGTGCGAATTGAAGCCCCAGTAAACGGCGGCCGTCTGTAGGCA2735040.15461444004825142No Hit
TTAGCGAAGTTCCTGATTCCACACTGCCACTGTAGGCACCATCAATTCGT2710870.15324808671668544No Hit
CCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA2700630.15266920967426775Illumina Single End Adapter 1 (95% over 22bp)
ACAACAGGTTGATATTCCTGTACCACCTCCCTGTAGGCACCATCAATTCG2692430.15220565579264422No Hit
ATCAGAGGGTCGAAGGTTCGAGTCCTTCATGGCTCACCACTGTAGGCACC2687850.15194674399046912No Hit
AAGGCTGAGCTGTGATGGCGAGCGCTGTAGGCACCATCAATTCGTATGCC2630890.14872674044202067No Hit
ACGGCGGTAACACGGGTTCGAATCCCGTACGGGTCCTGTAGGCACCATCA2455470.13881007923294722No Hit
TTAGCGAAGGTGCGAATTGAAGCCCCAGTAAACGGCGGCCGCTGTAGGCA2410260.13625431447828862No Hit
AAGCGGCGCGAGGGTTCAAATCCCTCCTTCTCCGCCACTGTAGGCACCAT2393540.13530911680746596No Hit
AAGAGTCTTGGAGGTAGAGCACTGATTGGACTAGGGGCCCCTGTAGGCAC2309880.13057973659568234No Hit
TAAGCGGCGCGAGGGTTCAAATCCCTCCTTCTCCGCCACTGTAGGCACCA2236950.1264569335972915No Hit
AAAAGGGCTCGCAGGCGGTTTCTTAAGTCTGACTGTAGGCACCATCAATT2211510.12501878594503502No Hit
GAAGCGAAGGTGCGAATTGAAGCCCCAGTAAACGGCGGCCGTCTGTAGGC2157830.121984203949236No Hit
TGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA2148180.12143868017391073Illumina Single End Adapter 1 (95% over 22bp)
ACCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA2099220.1186709243148511Illumina Single End Adapter 1 (95% over 22bp)
TTCGCTGGGTAGCTATGTGCGGACGGGCTGTAGGCACCATCAATTCGTAT2055700.1162106968845759No Hit
TTAGCGAAGGTGCGAATTGAAGCCCCAGTAAACGGCGGCCGTACTGTAGG2026330.11455038255490718No Hit
ATGACGTGGGGGTTCAAGTCCCTTCACCCGCACCACTGTAGGCACCATCA2020820.11423889695884064No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAG1916230.10832632372969347Illumina Single End Adapter 1 (95% over 22bp)
ATGATCAGGTTGATAGGTCTGAGGTGGACTGTAGGCACCATCAATTCGTA1900710.10744896321227915No Hit
GAGCGAAGGTGCGAATTGAAGCCCCAGTAAACGGCGGCCGTCTGTAGGCA1834590.10371113605948051No Hit
ACGATGAGTGCTAAGTGTTAGGGGGCTGTAGGCACCATCAATTCGTATGC1807670.10218932258468712No Hit
AGCGAAGTTCCTGATTCCACACTGCCACTGTAGGCACCATCAATTCGTAT1793950.1014137177973853No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)