FastQCFastQC Report
Tue 27 Jun 2023
SRR407282.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR407282.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42246607
Total Bases2.1 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTTGCCCCGAGATGAGTTCTCCCTGACCCCTGTAGGCACCATCAATTCG14182713.357124040754326No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA7736741.8313281348251234Illumina Single End Adapter 1 (95% over 22bp)
TTTGCCCCGAGATGAGTTCTCCCTGACCCCTGTAGGCACCATCAATTCGT6881481.6288834745947764No Hit
TAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGCA5551251.3140108506228678No Hit
ATGGGAAACAGGTTAATATTCCTGTACTTGGTGCTGTAGGCACCATCAAT4764001.127664524632712No Hit
AACTTGCCCCGAGATGAGTTCTCCCTGACCCCTGTAGGCACCATCAATTC4381391.0370986716163977No Hit
TATTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAAT4166170.986154935472096No Hit
AGCACTGTGCAAACACGAAAGTGGACGTATACGGTGCTGTAGGCACCATC3606440.8536638220437441No Hit
ACCGGAGTGGACGCATCACTGGTGTTCGGGTCTGTAGGCACCATCAATTC3212910.7605131460616471No Hit
AGGCACGCTGATATGTAGGTGAAGCGACCTGTAGGCACCATCAATTCGTA3209270.7596515384063861No Hit
ATGGGAAACAGGTTAATATTCCTGTACTTGGTGTTACCTGTAGGCACCAT2956140.6997343005557819No Hit
AGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGCAC2411100.5707203894504475No Hit
TAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGCTGTAGGCAC2238100.5297703552855736No Hit
TTGGGAAACAGGTTAATATTCCTGTACTTGGTGCTGTAGGCACCATCAAT2229860.5278199027912467No Hit
AAAACCGGGAGATAGCTGGTTCTCCCCGAAAGCTCTGTAGGCACCATCAA2142880.5072312671169071No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAT1936320.4583373997348474Illumina Single End Adapter 1 (95% over 22bp)
TGGGCGTTAAGTTGCAGGGTATAGACCCGCTGTAGGCACCATCAATTCGT1895000.448556732615237No Hit
TAGGCACGCTGATATGTAGGTGAAGCGACCTGTAGGCACCATCAATTCGT1855100.43911218716333833No Hit
TAACTTGCCCCGAGATGAGTTCTCCCTGACCCCTGTAGGCACCATCAATT1772210.4194916765741684No Hit
TTGCCCCGAGATGAGTTCTCCCTGACCCCTGTAGGCACCATCAATTCGTA1735540.4108116895636139No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAC1728740.40920209284499465Illumina Single End Adapter 1 (95% over 22bp)
ATGTACACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAATTCGTA1488800.35240699921771235No Hit
TTAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGC1464960.34676394248655285No Hit
ACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCACTGTAGGCACCATC1427400.33787328767017905No Hit
CCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA1305740.309075708730881Illumina Single End Adapter 1 (95% over 22bp)
ACTTGCCCCGAGATGAGTTCTCCCTGACTCCCTGTAGGCACCATCAATTC1257500.2976570402446757No Hit
TTGGGAAACAGGTTAATATTCCTGTACTTGGTGTTACCTGTAGGCACCAT1248750.29558586799645237No Hit
AAACTTGCCCCGAGATGAGTTCTCCCTGACCCCTGTAGGCACCATCAATT1214350.28744320224343695No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAACA1162570.27518659664195044Illumina Single End Adapter 1 (95% over 22bp)
TCAAACCGGGAGATAGCTGGTTCTCCCCGAAAGCTCTGTAGGCACCATCA1115950.2641513909034162No Hit
AAACCGGGAGATAGCTGGTTCTCCCCGAAAGCTCTGTAGGCACCATCAAT1112630.26336552897609034No Hit
ACCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA1085950.25705022890950746Illumina Single End Adapter 1 (95% over 22bp)
TACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCACTGTAGGCACCAT1009140.23886888715110305No Hit
TTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCACTGTAGGCAC993630.2351975864002522No Hit
TTTGCTCGTGGAGCTGAAATCAGTCGAAGATACCAGCTGGCCTGTAGGCA979630.23188371080309478No Hit
AGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGCTGTAGGCACC976530.23114992406372423No Hit
AGGTAACATCAAATCGTACCCCAAACCGCTGTAGGCACCATCAATTCGTA962140.227743733360646No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAG941800.22292914552877585Illumina Single End Adapter 1 (95% over 22bp)
ACCGCCAGCTAAGGTCCCAAAGTCATGGTTAAGTGGCTGTAGGCACCATC898700.21272714279752689No Hit
GGGGCTGATTCTGGATTCGACGGGATTTGCGCTGTAGGCACCATCAATTC875740.20729238681818873No Hit
AGACCGCCAGCTAAGGTCCCAAAGTCATGGTTAAGTGGCTGTAGGCACCA843770.19972491518667995No Hit
TGGGGCTGATTCTGGATTCGACGGGATTTGCGCTGTAGGCACCATCAATT824810.19523698080652963No Hit
TTGGGCGTTAAGTTGCAGGGTATAGACCCGCTGTAGGCACCATCAATTCG816440.1932557566102291No Hit
ATAGGGGAGCCGAAGGGAAACCGAGTCTTCTGTAGGCACCATCAATTCGT809550.19162485640562804No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAATA806990.1910188905821478Illumina Single End Adapter 1 (95% over 22bp)
ACCGCCAGCTAAGGTCCCAAAGTCATGGCTGTAGGCACCATCAATTCGTA792060.18748487896317922No Hit
CAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGCA782720.18527405052907564No Hit
AAGGCACGCTGATATGTAGGTGAAGCGACCTGTAGGCACCATCAATTCGT744890.17631948525475666No Hit
GGGGGCTGATTCTGGATTCGACGGGATTTGCGCTGTAGGCACCATCAATT739580.17506257958183483No Hit
TTGCCGTGGGCGCTGGAGAACTGAGGGGGGCTGCTCCCTGTAGGCACCAT725170.17165165477076064No Hit
TGCTCGTGGAGCTGAAATCAGTCGAAGATACCAGCTGGCCTGTAGGCACC720360.17051310179773727No Hit
CTTGCCCCGAGATGAGTTCTCCCTGACCCCTGTAGGCACCATCAATTCGT709630.1679732528579159No Hit
TAAACCGGGAGATAGCTGGTTCTCCCCGAAAGCTCTGTAGGCACCATCAA688350.16293616195023664No Hit
TCGACTTCGAAGGTTCGAATCCTTCCCCCACCACCACTGTAGGCACCATC650610.15400290016189938No Hit
TTAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGCTGTAGGCA647790.15333539093447196No Hit
TTTGCCCCGAGATGAGTTCTCCCTGACTCCCTGTAGGCACCATCAATTCG622460.14733964315761502No Hit
ATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCACTGTAGGCACCATCAAT620970.14698695211191753No Hit
GCGAAGGTGGCGGAATTGGTAGACGCGCCTGTAGGCACCATCAATTCGTA591160.13993076414397018No Hit
CGCAGGTTCGAATCCTGCACGACCCACCACTGTAGGCACCATCAATTCGT581510.13764655703592954No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAATC567340.13429244152080663Illumina Single End Adapter 1 (95% over 22bp)
TCCCCATGCGAGAGTAGGGAACTGCCAGGCATCTGTAGGCACCATCAATT541910.12827302320396997No Hit
CATTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAAT541170.12809786120812022No Hit
CGGTGGTTCAAGTCCACTCAGGCCCTGTAGGCACCATCAATTCGTATGCC532550.12605746066187043No Hit
GAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGCA520130.1231175795963922No Hit
TAGTCGATGGGAAACAGGTTAATATTCCTGTACTTGGTGCTGTAGGCACC501220.11864148048623172No Hit
TAACCGGGAGATAGCTGGTTCTCCCCGAAAGCTCTGTAGGCACCATCAAT497900.1178556185589058No Hit
GATTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAAT497870.1178485173969119No Hit
AGTCGATGGGAAACAGGTTAATATTCCTGTACTTGGTGCTGTAGGCACCA495580.11730646203137686No Hit
ATCTGCCGTGGGCGCTGGAGAACTGAGGGGGGCTGCTCCCTGTAGGCACC491460.11633123578421338No Hit
ATTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAATT474170.11223859942172398No Hit
TAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGCACCAT468330.11085623988690972No Hit
ACTTGCCCCGAGATGAGTTCTCCCTGACCCTCTGTAGGCACCATCAATTC451380.10684408336035127No Hit
ATGGGAAACAGGTTAATATTCCTGTACTTGGTGTCTGTAGGCACCATCAA445670.10549249552751065No Hit
TCTGCCGTGGGCGCTGGAGAACTGAGGGGGGCTGCTCCCTGTAGGCACCA438530.10380241897296036No Hit
TGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA437500.1035586124111695Illumina Single End Adapter 1 (95% over 22bp)
AGACCGCCAGCTAAGGTCCCAAAGTCATGGTTAAGTGGGCTGTAGGCACC435180.10300945588364054No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGA434540.1028579644277705Illumina Single End Adapter 1 (95% over 22bp)
TAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCCTGTAGGCACC427980.10130517700510246No Hit
TGGGCGTTAAGTTGCAGGGTATAGACCCCTGTAGGCACCATCAATTCGTA422590.10002933490019683No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)