FastQCFastQC Report
Tue 27 Jun 2023
SRR407283.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR407283.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46338235
Total Bases2.3 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGCA9019931.946541554722574No Hit
TATTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAAT5886951.2704303476384027No Hit
AGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGCAC5709681.2321746825272908No Hit
ACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCACTGTAGGCACCATC5180431.117960146734117No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA4918111.0613503082281837Illumina Single End Adapter 1 (95% over 22bp)
GGGGCTGATTCTGGATTCGACGGGATTTGCGCTGTAGGCACCATCAATTC4720711.0187504983735354No Hit
TGGGGCTGATTCTGGATTCGACGGGATTTGCGCTGTAGGCACCATCAATT3270390.7057649045113609No Hit
GGGGGCTGATTCTGGATTCGACGGGATTTGCGCTGTAGGCACCATCAATT3093760.6676473542852894No Hit
TACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCACTGTAGGCACCAT3047270.6576146027141517No Hit
ATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCACTGTAGGCACC2474280.5339607777464981No Hit
ATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCACTGTAGGCACCATCAAT2150300.4640444332849536No Hit
TTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCACTGTAGGCAC2077700.44837702601318324No Hit
TAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGCTGTAGGCAC1885830.4069706150870873No Hit
ACTTGCCCCGAGATGAGTTCTCCCTGACCCCTGTAGGCACCATCAATTCG1826550.3941777238602204No Hit
GCGAAGGTGGCGGAATTGGTAGACGCGCCTGTAGGCACCATCAATTCGTA1707070.36839340125924086No Hit
AGGGGCTGATTCTGGATTCGACGGGATTTGCGCTGTAGGCACCATCAATT1507320.32528645081108504No Hit
AGGCACGCTGATATGTAGGTGAAGCGACCTGTAGGCACCATCAATTCGTA1393990.30082932593354067No Hit
TTAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGC1374160.296549922542367No Hit
CGGTGGTTCAAGTCCACTCAGGCCCTGTAGGCACCATCAATTCGTATGCC1232550.2659898461820999No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAT1221280.26355772937834165Illumina Single End Adapter 1 (95% over 22bp)
AGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGCTGTAGGCACC1208800.2608644891200539No Hit
CAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGCA1140060.24603008724868353No Hit
TCGACTTCGAAGGTTCGAATCCTTCCCCCACCACCACTGTAGGCACCATC1102590.2379438923385839No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAC1078460.2327365295635451Illumina Single End Adapter 1 (95% over 22bp)
ATGTACACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAATTCGTA1059080.2285542382009155No Hit
TTTGCCCCGAGATGAGTTCTCCCTGACCCCTGTAGGCACCATCAATTCGT1051000.22681053777728047No Hit
AGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCCTGTAGGCACCATCA1023540.2208845459910158No Hit
CGCAGGTTCGAATCCTGCACGACCCACCACTGTAGGCACCATCAATTCGT1019450.22000190555380456No Hit
AGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTGTAGGCACC1017700.2196242476650222No Hit
AAGGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATTCGTATG964700.20818660874761413No Hit
TAGGGGCTGATTCTGGATTCGACGGGATTTGCGCTGTAGGCACCATCAAT956750.20647096291000294No Hit
TACTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAAT922010.19897391430640377No Hit
TGTCACTGGTTCGAGTCCAGTCAGAGGAGCCACTGTAGGCACCATCAATT872190.18822253372404021No Hit
CGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCACTGTAGGCACCATCA860140.18562208940413893No Hit
TTGTCGTGGGTTCGAATCCCATTAGCCACCCCACTGTAGGCACCATCAAT819910.1769402740523026No Hit
CGCTGTGCGGGTTCAAGTCCCGCTCCGGGTACCACTGTAGGCACCATCAA790200.17052872212331782No Hit
CCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA780850.16851094997468072Illumina Single End Adapter 1 (95% over 22bp)
AACTTGCCCCGAGATGAGTTCTCCCTGACCCCTGTAGGCACCATCAATTC778620.1680297059221181No Hit
AGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCTGTAGGCACCAT757170.16340069922818598No Hit
CATTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAAT741780.1600794678519801No Hit
GAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGCA734380.15848251449370052No Hit
ACCAGTGGGTCGGAGGTTCGAATCCTCTCTCGCCGACCACTGTAGGCACC726450.15677118474624682No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAACA717490.1548375763556812Illumina Single End Adapter 1 (95% over 22bp)
AAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGCACCATC711310.15350390449700985No Hit
ACCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA705930.15234287624463902Illumina Single End Adapter 1 (95% over 22bp)
CGGTGGTTCAAGTCCACTCAGGCCTCTGTAGGCACCATCAATTCGTATGC672980.14523211771013722No Hit
ACTTCGAAGGTTCGAATCCTTCCCCCACCACCACTGTAGGCACCATCAAT671710.14495804598513518No Hit
TAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCCTGTAGGCACC670820.14476597997312587No Hit
ATGGGAAACAGGTTAATATTCCTGTACTTGGTGCTGTAGGCACCATCAAT667710.14409482795363265No Hit
TAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCTGTAGGCACCA666010.14372796029024412No Hit
GATTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAAT663880.14326829668846905No Hit
TAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGCACCAT655130.14138000724455732No Hit
ACGAAGGGGTCGGAGGTTCGAATCCTCTATCACCGACCACTGTAGGCACC653920.14111888379002782No Hit
ATTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAATT646550.13952840456698448No Hit
TTTGTCGTGGGTTCGAATCCCATTAGCCACCCCACTGTAGGCACCATCAA642150.13857886473233175No Hit
TAGGCACGCTGATATGTAGGTGAAGCGACCTGTAGGCACCATCAATTCGT621570.1341376079602514No Hit
GCGAAGGTGGCGGAATTGGTAGACGCGCTGTAGGCACCATCAATTCGTAT620940.13400165112028975No Hit
ACGCAGGGGGTCGCGGGTTCGAGTCCCGTCCGTTCCGCCTGTAGGCACCA618710.1335204070677271No Hit
CACAGGTTCGAATCCCGTCGTAGCCACCACTGTAGGCACCATCAATTCGT611960.13206372663956664No Hit
AGCGAAGGTGGCGGAATTGGTAGACGCGCCTGTAGGCACCATCAATTCGT611850.13203998814370033No Hit
AGCACTGTGCAAACACGAAAGTGGACGTATACGGTGCTGTAGGCACCATC609160.1314594740175149No Hit
AGCGGGAATAGCTCAGTTGGTAGAGCACGACCCTGTAGGCACCATCAATT581330.1254536345633363No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAG580730.12532415185861093Illumina Single End Adapter 1 (95% over 22bp)
ACGCAGGAGGTCTGCGGTTCGATCCCGCATAGCTCCCTGTAGGCACCATC577890.12471126705624416No Hit
TAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGGCTGTAGGCA572900.12363440256194479No Hit
TAGACTTCGAAGGTTCGAATCCTTCCCCCACCACCACTGTAGGCACCATC567730.12251869325622781No Hit
TTCGCGCTGTGCGGGTTCAAGTCCCGCTCCGGGTACCACTGTAGGCACCA565190.12197054980622375No Hit
AGCTCAGTAGGTAGAGCAACTGCTGTAGGCACCATCAATTCGTATGCCGT560480.12095411057412954No Hit
TTTGCTCGTGGAGCTGAAATCAGTCGAAGATACCAGCTGGCCTGTAGGCA559420.12072535779578139No Hit
AATTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAAT554090.1195751197688043No Hit
AGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCCTGTAGGCACCATCA547030.11805153994320242No Hit
ATGGGAAACAGGTTAATATTCCTGTACTTGGTGTTACCTGTAGGCACCAT539550.11643732222429275No Hit
TAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGGA516250.11140907719079073No Hit
GCGGGAATAGCTCAGTTGGTAGAGCACGACCCTGTAGGCACCATCAATTC514100.11094509749885813No Hit
TGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCACTGTAGGCACC512530.1106062844214934No Hit
TCCCCATGCGAGAGTAGGGAACTGCCAGGCATCTGTAGGCACCATCAATT512000.11049190803231931No Hit
ATGAAAGCTACTAAACTGGTACTGGGCGCTGTAGGCACCATCAATTCGTA508970.10983802037345616No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAATA505210.10902659542384383Illumina Single End Adapter 1 (95% over 22bp)
ACGCAGGAGGTCTGCGGTTCGATCCCGCATAGCTCCACCACTGTAGGCAC503860.10873525933821172No Hit
TAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGGAGGCA500760.10806626536379729No Hit
ACCTGCGGTTGGATCACCTCCCTGTAGGCACCATCAATTCGTATGCCGTC499420.10777708732324397No Hit
AGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGGAC498550.10758933740139216No Hit
TGCATCCGGGGTTCGAATCCCCGCCTCACCGCCACTGTAGGCACCATCAA471750.10180577659032546No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)