FastQCFastQC Report
Mon 10 Jul 2023
SRR4293693_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4293693_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences178964457
Total Bases13.6 Gbp
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCGG30519991.7053659990151004No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACACTGTAGGCACCATCAA21660611.2103302724518088No Hit
GCGAGCGCACGGGGTCGGCGGCGACCTGTAGGCACCATCAATAGATCGGA19855951.1094912550149554No Hit
AGCGAGCGCACGGGGTCGGCGGCGACCTGTAGGCACCATCAATAGATCGG16126550.9011035079440384No Hit
AGCGAGCGCACGGGGTCGGCGGCGACTGTAGGCACCATCAATAGATCGGA13485890.7535513043240759No Hit
ACCTCTAGCATCACCAGTATTAGAGGCCTGTAGGCACCATCAATAGATCG9086420.5077220444951257No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACCTGTAGGCACCATCAAT7907260.4418341011701558No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACACTGTAGGCACCATCA6433850.3595043456031049No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCCTGTAGGCACCATCAATAGATCG6405290.3579084979985719No Hit
TGTCCGGTGCGGAGTGCCCTTCGTCCTGGGACTGTAGGCACCATCAATAG5796530.3238928051506898No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCGCTGTAGGCACCATCAATAGATC5741370.32081062889487605No Hit
GCGAGCGCACGGGGTCGGCGGCGACTGTAGGCACCATCAATAGATCGGAA5040150.28162854705836926No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCCTGTAGGCACCATCAA4620100.25815740608203563No Hit
TGAAAGTCAGCCCTCGACACAAGGGTTTGTCTGTAGGCACCATCAATAGA3937970.22004201649939908No Hit
AAGTCAGCCCTCGACACAAGGGTTTGTCTGTAGGCACCATCAATAGATCG3735310.20871798024118274No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACTGTAGGCACCATCAATAGA3680820.20567324158673586No Hit
TTGAAAGTCAGCCCTCGACACAAGGGTTTGTCTGTAGGCACCATCAATAG3532550.19738835628126983No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACTGTAGGCACCATCAATA3518110.19658149215628887No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCGACTGTAGGCACCATCAATAGAT2949220.16479361597481895No Hit
ACCGGGGTCCGGTGCGGAGTGCCCTTCCTGTAGGCACCATCAATAGATCG2587080.14455831305095404No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACCTGTAGGCACCATCAA2515660.1405675764992822No Hit
GCGTTTACTTTGAAAAAATTAGAGTGTTCACTGTAGGCACCATCAATAGA2331860.1302973807810341No Hit
GCTGCGATCTATTGAAAGTCAGCCCTCGACACACTGTAGGCACCATCAAT2258400.12619265511475275No Hit
TGCGAGCGCACGGGGTCGGCGGCGACCTGTAGGCACCATCAATAGATCGG2092850.11694221495612395No Hit
TCTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCG2084240.11646111384005149No Hit
AGTCAGCCCTCGACACAAGGGTTTGTCTGTAGGCACCATCAATAGATCGG2045920.11431990654993578No Hit
AAGTCAGCCCTCGACACAAGGGTTTGCTGTAGGCACCATCAATAGATCGG1988470.11110977192527118No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCTGTAGGCACCATCAAT1957410.10937423177832456No Hit
ATGTACACGGAGTCGAGCTCAACCCGCAACGCGACTGTAGGCACCATCAA1929360.10780688145244394No Hit
TGGAATGAGTCCACTTTAAATCCTTTACTGTAGGCACCATCAATAGATCG1885560.1053594681093576No Hit
TTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCTGTAGGCACCAT1870610.10452410670572425No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)