FastQCFastQC Report
Mon 3 Jul 2023
SRR4293694_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4293694_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences184823119
Total Bases14 Gbp
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCGG35993701.9474674053087484No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACACTGTAGGCACCATCAA20408091.1041957364651984No Hit
AGCGAGCGCACGGGGTCGGCGGCGACTGTAGGCACCATCAATAGATCGGA17120920.926340822113277No Hit
AGCGAGCGCACGGGGTCGGCGGCGACCTGTAGGCACCATCAATAGATCGG12427760.6724137146500595No Hit
ATGTACACGGAGTCGACCCAACGCGACTGTAGGCACCATCAATAGATCGG11668870.6313533752235834No Hit
GCGAGCGCACGGGGTCGGCGGCGACCTGTAGGCACCATCAATAGATCGGA10939460.5918880743485343No Hit
ACCTCTAGCATCACCAGTATTAGAGGCCTGTAGGCACCATCAATAGATCG10193230.5515127141642924No Hit
ATGTACACGGAGTCGAGCTCAACCCGCAACGCGACTGTAGGCACCATCAA7739530.41875334870850217No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACACTGTAGGCACCATCA6738060.36456802787750814No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCCTGTAGGCACCATCAA6038660.3267264416201092No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACCTGTAGGCACCATCAAT5810780.31439681525989177No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCCTGTAGGCACCATCAATAGATCG5632900.3047724781660026No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCGCTGTAGGCACCATCAATAGATC5582670.3020547445690493No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCGACTGTAGGCACCATCAATAGAT3443830.18633112668118107No Hit
TGGATAACTGTGGTAATTCTAGAGCTAATACATGCTGTAGGCACCATCAA3346510.18106555165319985No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACTGTAGGCACCATCAATAGA3203670.17333708127715342No Hit
GCGAGCGCACGGGGTCGGCGGCGACTGTAGGCACCATCAATAGATCGGAA3087560.16705485854288607No Hit
GTCCGGTGCGGAGTGCCCTTCGTCCTGGGACTGTAGGCACCATCAATAGA3014870.16312190900749812No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACTGTAGGCACCATCAATA2869470.15525492782101571No Hit
ACCGGGGTCCGGTGCGGAGTGCCCTTCCTGTAGGCACCATCAATAGATCG2782860.15056882575388203No Hit
TGTCCGGTGCGGAGTGCCCTTCGTCCTGGGACTGTAGGCACCATCAATAG2538620.137354028745722No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCTGTAGGCACCATCAAT2529540.1368627482149568No Hit
GCTGCGATCTATTGAAAGTCAGCCCTCGACACACTGTAGGCACCATCAAT2415380.13068603176207624No Hit
AGGCCCGGCGGGGAAGGTGGCTCGGGGGGCTGTAGGCACCATCAATAGAT2281450.12343964393328954No Hit
ATTGATCATCGACACTTCGAACGCACTGTAGGCACCATCAATAGATCGGA2212680.11971878907638173No Hit
AAGGGACAAGCATCAAGCACGCAGCCTGTAGGCACCATCAATAGATCGGA2187640.11836398021180455No Hit
TTGGATAACTGTGGTAATTCTAGAGCTAATACATGCTGTAGGCACCATCA2125710.11501320892653044No Hit
AAGTCAGCCCTCGACACAAGGGTTTGTCTGTAGGCACCATCAATAGATCG2018230.10919791911963134No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACCTGTAGGCACCATCAA1982400.10725930883138056No Hit
TTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCTGTAGGCACCATC1911680.10343294769308595No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)