FastQCFastQC Report
Thu 13 Jul 2023
SRR4293695_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4293695_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences199563521
Total Bases15.1 Gbp
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACACTGTAGGCACCATCAA42003542.1047704404854635No Hit
CTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCGG41087902.058888307547951No Hit
AGCGAGCGCACGGGGTCGGCGGCGACCTGTAGGCACCATCAATAGATCGG18287690.9163844127604865No Hit
ACCTCTAGCATCACCAGTATTAGAGGCCTGTAGGCACCATCAATAGATCG17356640.8697300946098261No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACACTGTAGGCACCATCA14127740.7079319872292692No Hit
AGCGAGCGCACGGGGTCGGCGGCGACTGTAGGCACCATCAATAGATCGGA11934160.5980131008011228No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACCTGTAGGCACCATCAAT11555810.5790542250454681No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCCTGTAGGCACCATCAA10820860.5422263520796469No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCGCTGTAGGCACCATCAATAGATC10051070.5036526690667078No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCCTGTAGGCACCATCAATAGATCG7570500.3793528978675416No Hit
GTCCGGTGCGGAGTGCCCTTCGTCCTGGGACTGTAGGCACCATCAATAGA7319520.36677645109298307No Hit
TGTCCGGTGCGGAGTGCCCTTCGTCCTGGGACTGTAGGCACCATCAATAG6454440.32342784731684504No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA5217370.2614390633045605Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACTGTAGGCACCATCAATA5057320.2534190604905192No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCGACTGTAGGCACCATCAATAGAT4832960.24217652483692148No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCTGTAGGCACCATCAAT4698930.235460367528793No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACCTGTAGGCACCATCAA4698130.2354202800420624No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACTGTAGGCACCATCAATAGA4471790.2240785278588064No Hit
GCTGCGATCTATTGAAAGTCAGCCCTCGACACACTGTAGGCACCATCAAT4386580.219808709428413No Hit
AGGCCCGGCGGGGAAGGTGGCTCGGGGGGCTGTAGGCACCATCAATAGAT3706240.1857173085255396No Hit
AGAGAAATAAGGCCTACTTCACAAAGCGCCTTCCCCTGTAGGCACCATCA3666750.1837384899618002No Hit
TGGATAACTGTGGTAATTCTAGAGCTAATACATGCTGTAGGCACCATCAA3655900.18319480342301636No Hit
TCACCTCTAGCATCACCAGTATTAGAGGCCTGTAGGCACCATCAATAGAT3517820.17627570321331423No Hit
GCGAGCGCACGGGGTCGGCGGCGACCTGTAGGCACCATCAATAGATCGGA3377470.169242854760014No Hit
ACCGGGGTCCGGTGCGGAGTGCCCTTCCTGTAGGCACCATCAATAGATCG3359050.16831984037804182No Hit
TGTGCATTTATCAGATCAAAACCAACCCGGCTGTAGGCACCATCAATAGA3217990.16125141428026818No Hit
CCCGCCACGCAGTTTTATCCGGTAAAGCGCTGTAGGCACCATCAATAGAT3103540.15551639820987123No Hit
CCTGCGGAAGGATCATTACTGTAGGCACCATCAATAGATCGGAAGAGCAC2877750.14420220617374255No Hit
CCCCGCCACGCAGTTTTATCCGGTAAAGCGCTGTAGGCACCATCAATAGA2662510.13341666786887368No Hit
TGTGCATTTATCAGATCAAAACCAACCTGTAGGCACCATCAATAGATCGG2642650.13242149601078645No Hit
TGACCGGGGTCCGGTGCGGAGTGCCCTTCCTGTAGGCACCATCAATAGAT2489380.12474123464678699No Hit
CGCCGGCCGAGGTGGGATCCCGAGGCCTGTAGGCACCATCAATAGATCGG2476250.12408329877082094No Hit
AGTCGGTAGAGCATGAGACTCTTAATCTCCTGTAGGCACCATCAATAGAT2462560.12339730165414349No Hit
CGATCAAAAGGGACAAGCATCAAGCACGCAGCCTGTAGGCACCATCAATA2133960.10693136647954814No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACTGTAGGCACCATCAATAG2132670.10686672540719502No Hit
TGGATAACTGTGGTAATTCTAGAGCTAATACACTGTAGGCACCATCAATA2114440.1059532318033214No Hit
AAGGGACAAGCATCAAGCACGCAGCCTGTAGGCACCATCAATAGATCGGA2083520.10440385044118358No Hit
TACTTGGATAACTGTGGTAATTCTAGAGCTCTGTAGGCACCATCAATAGA2050680.10275825911089231No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)