FastQCFastQC Report
Thu 13 Jul 2023
SRR4293696_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4293696_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences225042166
Total Bases17.1 Gbp
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCGG36450261.6197080150748282No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACACTGTAGGCACCATCAA25265621.12270604434193No Hit
AGCGAGCGCACGGGGTCGGCGGCGACCTGTAGGCACCATCAATAGATCGG16389910.7283039570459875No Hit
ACCTCTAGCATCACCAGTATTAGAGGCCTGTAGGCACCATCAATAGATCG11796700.524199540454121No Hit
AGCGAGCGCACGGGGTCGGCGGCGACTGTAGGCACCATCAATAGATCGGA10343270.4596147550410619No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACACTGTAGGCACCATCA8674320.38545309770969766No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACCTGTAGGCACCATCAAT8223500.36542040748043636No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCCTGTAGGCACCATCAATAGATCG6604650.29348499960669594No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA6396260.2842249572020205Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CCGCCACGCAGTTTTATCCGGTAAAGCGCTGTAGGCACCATCAATAGATC6230390.2768543384887257No Hit
GTCCGGTGCGGAGTGCCCTTCGTCCTGGGACTGTAGGCACCATCAATAGA6155370.27352074099748935No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCCTGTAGGCACCATCAA5842980.25963934243327536No Hit
TTGAAAGTCAGCCCTCGACACAAGGGTTTGTCTGTAGGCACCATCAATAG5336460.23713156049164583No Hit
TGAAAGTCAGCCCTCGACACAAGGGTTTGTCTGTAGGCACCATCAATAGA5179740.23016753224815653No Hit
AAGTCAGCCCTCGACACAAGGGTTTGTCTGTAGGCACCATCAATAGATCG5166400.22957475444846187No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACTGTAGGCACCATCAATAGA4670370.20753310737330888No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACTGTAGGCACCATCAATA4653870.20679991144415133No Hit
TGTCCGGTGCGGAGTGCCCTTCGTCCTGGGACTGTAGGCACCATCAATAG4038070.17943615064565277No Hit
GCGAGCGCACGGGGTCGGCGGCGACCTGTAGGCACCATCAATAGATCGGA3754300.1668265137476503No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCGACTGTAGGCACCATCAATAGAT3690720.16400126543396318No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACCTGTAGGCACCATCAA3322610.14764388643504256No Hit
TACTTGGATAACTGTGGTAATTCTAGAGCTCTGTAGGCACCATCAATAGA3217380.1429678738516941No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCTGTAGGCACCATCAAT2996840.1331679326264572No Hit
TCACCTCTAGCATCACCAGTATTAGAGGCCTGTAGGCACCATCAATAGAT2818380.1252378632011567No Hit
CCTGCGGAAGGATCATTACTGTAGGCACCATCAATAGATCGGAAGAGCAC2806750.12472107116139293No Hit
GCTGCGATCTATTGAAAGTCAGCCCTCGACACACTGTAGGCACCATCAAT2781380.12359372687516704No Hit
TTGAAAGTCAGCCCTCGACACAAGGGTTTCTGTAGGCACCATCAATAGAT2689690.11951937931489692No Hit
GCGTTTACTTTGAAAAAATTAGAGTGTTCACTGTAGGCACCATCAATAGA2643930.11748598260469996No Hit
ATTGAAAGTCAGCCCTCGACACAAGGGTTTGTCTGTAGGCACCATCAATA2573820.11437056644753411No Hit
AGTCAGCCCTCGACACAAGGGTTTGTCTGTAGGCACCATCAATAGATCGG2533230.1125669044618065No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACTGTAGGCACCATCAATAG2531410.11248603072901459No Hit
AGAGAAATAAGGCCTACTTCACAAAGCGCCTTCCCCTGTAGGCACCATCA2515680.11178705060988438No Hit
CGATCAAAAGGGACAAGCATCAAGCACGCAGCCTGTAGGCACCATCAATA2422980.10766782257152643No Hit
TGAAAGTCAGCCCTCGACACAAGGGTTTCTGTAGGCACCATCAATAGATC2421150.10758650447756533No Hit
TTGAAAGTCAGCCCTCGACACAAGGGTTTGCTGTAGGCACCATCAATAGA2418820.1074829683251449No Hit
GCGCAGCGGAAGCGTGCTGGGCCTGTAGGCACCATCAATAGATCGGAAGA2341440.10404450159798052No Hit
TGGATAACTGTGGTAATTCTAGAGCTAATACATGCTGTAGGCACCATCAA2313940.10282250838271793No Hit
TACTTGGATAACTGTGGTAATTCTAGAGCTAATACTGTAGGCACCATCAA2312010.10273674667706496No Hit
TATTGAAAGTCAGCCCTCGACACAAGGGTTTGTCTGTAGGCACCATCAAT2266040.10069401838231506No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)