FastQCFastQC Report
Mon 3 Jul 2023
SRR4417735.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4417735.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32704873
Total Bases1.6 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCATCCGTAGCTCAGCTGGATAGAGTACTCCTGTAGGCACCATCAATTCG2435700.7447514014195988No Hit
AGGCTTGTAGCTCAGGTGGTTAGAGCGCACCTGTAGGCACCATCAATTCG1503340.4596685026112164No Hit
ACAAGCGGTGGAGCATGTGGTTTAATTCGCTGTAGGCACCATCAATTCGT1298080.39690721318501987No Hit
TGTCCCCTTCGTCTAGAGGCCCAGGACACCGCTGTAGGCACCATCAATTC1169660.35764089345340067No Hit
TAGGCTTGTAGCTCAGGTGGTTAGAGCGCACCTGTAGGCACCATCAATTC1128220.3449699988133267No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA1056790.3231292168601297Illumina Single End Adapter 1 (95% over 22bp)
ACCGGAGTGGACGCATCACTGGTGTTCGGGTCTGTAGGCACCATCAATTC1024380.31321937865345023No Hit
GGTGAGGTGTCCGAGTGGCTGAAGGAGCACCTGTAGGCACCATCAATTCG982490.3004108898389546No Hit
GCATCCGCAGCTCAGCTGGATAGAGTACTCCTGTAGGCACCATCAATTCG978060.29905635163298144No Hit
GTCCCCTTCGTCTAGAGGCCCAGGACACCGCTGTAGGCACCATCAATTCG967580.2958519361931171No Hit
GCGGGAATAGCTCAGTTGGTAGAGCACGACTGTAGGCACCATCAATTCGT966390.2954880760429799No Hit
GGAGCGGTAGTTCAGTTGGTTAGAATACCCTGTAGGCACCATCAATTCGT876330.26795089526872645No Hit
GGAGCGGTAGTTCAGTTGGTTAGAATACCTGTAGGCACCATCAATTCGTA789290.24133712428725837No Hit
TAGGGTGAGGTCGGTGGTTCAAGTCCACTCAGGCCTACCACTGTAGGCAC721960.22074997814545863No Hit
CGCAGGTTCGAATCCTGCAGGGCGCGCCACTGTAGGCACCATCAATTCGT673090.20580725080326712No Hit
TAGGGTGAGGTCCCCAGTTCGACTCTGGGTATCAGCACCACTGTAGGCAC673070.20580113550662618No Hit
TGGGGCTGATTCTGGATTCGACGGGATTTGCGCTGTAGGCACCATCAATT651350.19915992335454105No Hit
TGCGGGCATCGTATAATGGCTATTACCTCACTGTAGGCACCATCAATTCG625950.1913934966205189No Hit
GGGGGCTGATTCTGGATTCGACGGGATTTGCGCTGTAGGCACCATCAATT595930.18221443636243442No Hit
GCGGGCATCGTATAATGGCTATTACCTCACTGTAGGCACCATCAATTCGT558290.1707054480841433No Hit
AGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCACTGTAGGCACC555420.1698279030161652No Hit
ACGCAGGAGGTCTGCGGTTCGATCCCGCATAGCTCCACCACTGTAGGCAC488130.1492529874676474No Hit
TAGGGTGAGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTGTAGGCAC480340.14687107942599256No Hit
GGGTGATTAGCTCAGCTGGGAGAGCACCTCTGTAGGCACCATCAATTCGT447890.13694901062603115No Hit
ATCAGTAGGTCACCAGTTCGATTCCGGTAGCTGTAGGCACCATCAATTCG440800.13478113796681004No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCGCTGTAGGCACCATCAATTCGT435960.13330123617969714No Hit
GCGCCCGTAGCTCAGCTGGATAGAGCGCTGCTGTAGGCACCATCAATTCG432390.13220965572928536No Hit
ACTTGCCCCGAGATGAGTTCTCCCTGACTGTAGGCACCATCAATTCGTAT430630.13167150962488067No Hit
ACGCAGGGGGTCGCGGGTTCGAGTCCCGTCCGTTCCTGTAGGCACCATCA407530.12460834200456916No Hit
TTTTCGGACGCGGGTTCAACTCCCGCCAGCTCCACCACTGTAGGCACCAT405710.1240518500102416No Hit
GGGGCTGATTCTGGATTCGACGGGATTTGCGCTGTAGGCACCATCAATTC395780.1210156052280038No Hit
TGGTGAGGTGTCCGAGTGGCTGAAGGAGCACCTGTAGGCACCATCAATTC394070.12049274736520152No Hit
ATCTAGAGGGTATTAATAATGAATCGTACTAAACCTGTAGGCACCATCAA383060.11712627656435175No Hit
TTAGGTTAATGAGGCGAACCGGGGGAACTGCTGTAGGCACCATCAATTCG366440.11204446505571203No Hit
ACGCAGGAGGTCTGCGGTTCGATCCCGCATACTGTAGGCACCATCAATTC366350.1120169462208277No Hit
TCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGCTGTAGGCACCATCAATT358600.10964726877245479No Hit
GCATCCGTAGCTCAGCTGGATAGAGTACTCTGTAGGCACCATCAATTCGT336920.10301828721365161No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)