FastQCFastQC Report
Mon 3 Jul 2023
SRR4417737.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4417737.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31420973
Total Bases1.5 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGGAGCCCCTGTAGGCACC5353061.7036582539948715No Hit
TATTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAAT3856231.2272789897372052No Hit
ACCGGAGTGGACGCATCACTGGTGTTCGGGTCTGTAGGCACCATCAATTC2872720.9142683137151737No Hit
ACAAGCGGTGGAGCATGTGGTTTAATTCGCTGTAGGCACCATCAATTCGT2306780.7341529493692No Hit
AGGTCGGGAACTCAAAGGAGACTGCCAGCTGTAGGCACCATCAATTCGTA1827890.5817420103444919No Hit
AGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGGAGCCCTGTAGGCACCA1660870.528586431744173No Hit
TTTCGCCATTTAAAGTGGTACGCGAGCTGGGCTGTAGGCACCATCAATTC1578610.5024064658977938No Hit
AGGCTTGTAGCTCAGGTGGTTAGAGCGCACCTGTAGGCACCATCAATTCG1471810.4684164300067983No Hit
AAAATGCCCTGCTTCCAGGAAAAGCCTCCTGTAGGCACCATCAATTCGTA1094020.3481814519238472No Hit
TATAGGGGAGCCGGAGGGAAACCGAGTCTTCTGTAGGCACCATCAATTCG1090760.34714392835638797No Hit
GCATCCGTAGCTCAGCTGGATAGAGTACTCCTGTAGGCACCATCAATTCG1082120.34439417264385797No Hit
AGGTCGGGAACTCAAAGGAGACTGCCACTGTAGGCACCATCAATTCGTAT974250.3100635998764265No Hit
AAAACCGGGAGATAGCTGGTTCTCCCCGAAAGCTCTGTAGGCACCATCAA970990.3090260763089673No Hit
ACCGCCAGCTAAGGTCCCAAAGTCATGCTGTAGGCACCATCAATTCGTAT929600.2958533461073914No Hit
ATAGGGGAGCCGGAGGGAAACCGAGTCTTCTGTAGGCACCATCAATTCGT866150.27565982759349944No Hit
TTCGCCATTTAAAGTGGTACGCGAGCTGGGCTGTAGGCACCATCAATTCG797560.2538304590376625No Hit
AAAGGGGGGACGGAGAAGGCTATGTTGGCCGCTGTAGGCACCATCAATTC778680.2478217335917637No Hit
TTGAAGCAACAAATGCCCTGCTTCCAGGAAAAGCCTCCTGTAGGCACCAT717850.2284620530369954No Hit
TAGGCTTGTAGCTCAGGTGGTTAGAGCGCACCTGTAGGCACCATCAATTC706220.22476070362302275No Hit
TTTCGCCATTTAAAGTGGTACGCGAGCTGGGTCTGTAGGCACCATCAATT692290.2203273590540942No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCGCTGTAGGCACCATCAATTCGT673310.21428680773189296No Hit
GCGGGAATAGCTCAGTTGGTAGAGCACGACTGTAGGCACCATCAATTCGT615880.19600920697140728No Hit
TTGAGCTAACCGGTACTAATGAACCGTGAGGCTTAACCTGTAGGCACCAT615500.19588826864145806No Hit
TTGGGAAACGGGTTAATATTCCCGTACTTGGTGTCTGTAGGCACCATCAA609560.1939978115890937No Hit
TGGCCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGCTGTAGGCACCAT591570.1882723364422865No Hit
ATGGGAAACGGGTTAATATTCCCGTACTTGGTGTCTGTAGGCACCATCAA579740.184507335275709No Hit
TCAAACCGGGAGATAGCTGGTTCTCCCCGAAAGCTGTAGGCACCATCAAT563510.1793419955518246No Hit
AAAATGCCCTGCTTCCAGGAAAAGCCTCTCTGTAGGCACCATCAATTCGT508690.16189505016283232No Hit
AGGTTAATGAGGCGAACCGGGGGAACTGACTGTAGGCACCATCAATTCGT504380.16052335489419758No Hit
TTTGAGCTAACCGGTACTAATGAACCGTGAGGCCTGTAGGCACCATCAAT467590.14881461500253348No Hit
GGAAGTGTGGCCGAGCGGTTGAAGGCACCGCTGTAGGCACCATCAATTCG462460.1471819475482188No Hit
TAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGGAGCCCCTGTAGGCAC412780.13137085220117148No Hit
TCTGCGAAGGGGGGACGGAGAAGGCTATGTTGGCCGCTGTAGGCACCATC409470.13031741569556105No Hit
GGTGAGGTGTCCGAGTGGCTGAAGGAGCACCTGTAGGCACCATCAATTCG395960.12601773980710274No Hit
TTAGGTTAATGAGGCGAACCGGGGGAACTGACTGTAGGCACCATCAATTC395790.1259636358173886No Hit
GCATCCGCAGCTCAGCTGGATAGAGTACTCCTGTAGGCACCATCAATTCG385200.12259327551696123No Hit
ATGGGAAACGGGTTAATATTCCCGTACTTGGTGCTGTAGGCACCATCAAT378910.12059142789753838No Hit
TTGGGAAACGGGTTAATATTCCCGTACTTGGTGCTGTAGGCACCATCAAT376610.11985943274258248No Hit
TAAACCGGGAGATAGCTGGTTCTCCCCGAAAGCTCTGTAGGCACCATCAA374000.11902877737108905No Hit
TCAAACCGGGAGATAGCTGGTTCTCCCCGAAAGCTCTGTAGGCACCATCA371680.11829041704087266No Hit
AGCTCCTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGCACCATCA370710.1179817060407391No Hit
TTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCTGTAGGCACCATCAAT364990.11616126591624008No Hit
AAAGGGGGGACGGAGAAGGCTATGTTGGCCGGGCCTGTAGGCACCATCAA357000.11361837839967591No Hit
TTGAAGCAACAAATGCCCTGCTTCCAGGAAAAGCCTCTCTGTAGGCACCA356890.11358336993574324No Hit
CATTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAAT356060.11331921516243305No Hit
AGCAGTGGGAGCACCCTTCGGGGTGTGACTGCGTACCCTGTAGGCACCAT349400.11119961180069121No Hit
TGAAGCAACAAATGCCCTGCTTCCAGGAAAAGCCTCCTGTAGGCACCATC344460.10962741351135116No Hit
ACCCAGACCGCCAGCTAAGGTCCCAAAGTCATGCTGTAGGCACCATCAAT344240.10955739658348582No Hit
TTAGGTTAATGAGGCGAACCGGGGGAACTGCTGTAGGCACCATCAATTCG343000.10916275571733569No Hit
GGTGGCTATAGCTCAGTTGGTAGAGCCCTGCTGTAGGCACCATCAATTCG336160.10698586577824944No Hit
AGCTCCTGATCGAAGCCCCGGTAAACGGCGGCCGTACTGTAGGCACCATC336010.10693812696379582No Hit
TCCCCATGCGAGAGTAGGGAACTGCCAGGCATCTGTAGGCACCATCAATT332270.10574783919008492No Hit
GGGTCGTTAGCTCAGTTGGTAGAGCAGTTCTGTAGGCACCATCAATTCGT328760.10463075093186962No Hit
TTGAGCTAACCGGTACTAATGAACCGTGAGGCCTGTAGGCACCATCAATT317950.10119037370357689No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)