FastQCFastQC Report
Mon 3 Jul 2023
SRR4417738.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4417738.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27566313
Total Bases1.3 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCATCCGTAGCTCAGCTGGATAGAGTACTCCTGTAGGCACCATCAATTCG4420101.6034425786284876No Hit
TAGGCTTGTAGCTCAGGTGGTTAGAGCGCACCTGTAGGCACCATCAATTC2521870.9148376135756711No Hit
AGGCTTGTAGCTCAGGTGGTTAGAGCGCACCTGTAGGCACCATCAATTCG2147700.7791031031244549No Hit
GCATCCGCAGCTCAGCTGGATAGAGTACTCCTGTAGGCACCATCAATTCG1849770.6710255375827736No Hit
ACAAGCGGTGGAGCATGTGGTTTAATTCGCTGTAGGCACCATCAATTCGT1421950.5158288669217389No Hit
GCGGGAATAGCTCAGTTGGTAGAGCACGACTGTAGGCACCATCAATTCGT1307670.47437247048598774No Hit
ACCGGAGTGGACGCATCACTGGTGTTCGGGTCTGTAGGCACCATCAATTC998450.36219932640248265No Hit
AGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCACTGTAGGCACC982380.35636974737970945No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCGCTGTAGGCACCATCAATTCGT964250.3497928794467363No Hit
GGGGGCTGATTCTGGATTCGACGGGATTTGCGCTGTAGGCACCATCAATT930710.3376258551515395No Hit
GCATCCGTAGCTCAGCTGGATAGAGTACTCTGTAGGCACCATCAATTCGT864770.31370535479300404No Hit
GGTGGCTATAGCTCAGTTGGTAGAGCCCTGCTGTAGGCACCATCAATTCG753590.27337351933862175No Hit
GCGCCCGTAGCTCAGCTGGATAGAGCGCTGCTGTAGGCACCATCAATTCG730860.2651279480139401No Hit
GGAAGTGTGGCCGAGCGGTTGAAGGCACCGCTGTAGGCACCATCAATTCG702090.2546912965836236No Hit
TGTCCCCTTCGTCTAGAGGCCCAGGACACCGCTGTAGGCACCATCAATTC694670.25199960546047634No Hit
TTAGGTTAATGAGGCGAACCGGGGGAACTGACTGTAGGCACCATCAATTC599660.21753362518955655No Hit
TGGGGCTGATTCTGGATTCGACGGGATTTGCGCTGTAGGCACCATCAATT592810.21504870818233834No Hit
TGGTGGCTATAGCTCAGTTGGTAGAGCCCTGCTGTAGGCACCATCAATTC586320.21269438535360172No Hit
GCGAAGGTGGCGGAATTGGTAGACGCGCTACTGTAGGCACCATCAATTCG568030.2060594755635257No Hit
AGGTTAATGAGGCGAACCGGGGGAACTGACTGTAGGCACCATCAATTCGT535310.19418991578598124No Hit
AGCAGTGGGAGCACCCTTCGGGGTGTGACTGCGTACCCTGTAGGCACCAT530450.19242689437648047No Hit
GCGTCTGTAGCTCAGTTGGTTAGAGCACCACTGTAGGCACCATCAATTCG521750.18927086839650992No Hit
GTCCCCTTCGTCTAGAGGCCCAGGACACCGCTGTAGGCACCATCAATTCG513310.18620916043433158No Hit
GCGGGAATAGCTCAGTTGGTAGAGCACGACCTGTAGGCACCATCAATTCG451990.16396461869964257No Hit
GGTGAGGTGTCCGAGTGGCTGAAGGAGCACCTGTAGGCACCATCAATTCG450930.1635800913963358No Hit
TGCGGGAATAGCTCAGTTGGTAGAGCACGACTGTAGGCACCATCAATTCG449010.16288358911110093No Hit
GCGGGCATCGTATAATGGCTATTACCTCACTGTAGGCACCATCAATTCGT446830.16209276880807383No Hit
TGCGGGCATCGTATAATGGCTATTACCTCACTGTAGGCACCATCAATTCG430760.15626318978530063No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA409920.14870323789764703Illumina Single End Adapter 1 (95% over 22bp)
TGGCCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGCTGTAGGCACCAT385070.13968861196635182No Hit
TCAAACCGGGAGATAGCTGGTTCTCCCCGAAAGCTCTGTAGGCACCATCA371680.13483123405005232No Hit
GGGTGATTAGCTCAGCTGGGAGAGCACCTCTGTAGGCACCATCAATTCGT370440.13438140965750478No Hit
TGCATCCGTAGCTCAGCTGGATAGAGTACTCCTGTAGGCACCATCAATTC355090.12881301899169467No Hit
GCATCCGCAGCTCAGCTGGATAGAGTACTCTGTAGGCACCATCAATTCGT349750.12687587201088518No Hit
TGCGCCCGTAGCTCAGCTGGATAGAGCGCTGCTGTAGGCACCATCAATTC347920.12621201827027068No Hit
CAGGCTTGTAGCTCAGGTGGTTAGAGCGCACCTGTAGGCACCATCAATTC340220.1234187538971933No Hit
AGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGGAGCCCCTGTAGGCACC324910.11786487369565891No Hit
TTGGGGTATCGCCAAGCGGTAAGGCACCGCTGTAGGCACCATCAATTCGT320510.11626872262532897No Hit
CGGTGATTGGCGCAGCCTGGTAGCGCACTTCTGTAGGCACCATCAATTCG319480.11589507817022901No Hit
AGAAGGCACGCTGACACGTAGGTGAAGTCTGTAGGCACCATCAATTCGTA318560.11556133749188728No Hit
ACCGCCAGCTAAGGTCCCAAAGTCATGCTGTAGGCACCATCAATTCGTAT313970.11389626171624766No Hit
GCTGATATGGCTCAGTTGGTAGAGCGCACCTGTAGGCACCATCAATTCGT300680.10907515996063746No Hit
AAAACCGGGAGATAGCTGGTTCTCCCCGAAAGCTCTGTAGGCACCATCAA298520.10829159488974821No Hit
TCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGCTGTAGGCACCATCAATT293310.10640160691783483No Hit
TCCTCTGCAGTTCAGTCGGTAGAACGGCGCTGTAGGCACCATCAATTCGT290740.10546930958811938No Hit
TTTCGCCATTTAAAGTGGTACGCGAGCTGGGCTGTAGGCACCATCAATTC289940.10517910030260484No Hit
TTAGGTTAATGAGGCGAACCGGGGGAACTGCTGTAGGCACCATCAATTCG283120.10270506614359345No Hit
GCTGATATAGCTCAGTTGGTAGAGCGCACCTGTAGGCACCATCAATTCGT283120.10270506614359345No Hit
TGCTGATATGGCTCAGTTGGTAGAGCGCACCTGTAGGCACCATCAATTCG279200.10128304064457223No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)