FastQCFastQC Report
Mon 3 Jul 2023
SRR4421280.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4421280.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20726589
Total Bases1 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAAT273356413.188682421405664No Hit
AAGGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATTCGTATG227595510.980846872584776No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA7105143.4280315009864863Illumina Single End Adapter 1 (95% over 22bp)
TACTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAAT5564292.684614434145435No Hit
CATTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAAT3613691.7435044425303172No Hit
TAAGGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATTCGTAT2805061.3533630642263423No Hit
ATTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAATT2551401.2309792026078192No Hit
GATTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAAT2545031.227905855613772No Hit
AAGGCTGAGGCGTGATGACGAGGCACTGTAGGCACCATCAATTCGTATGC2495571.204042787744766No Hit
AATTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAAT2293731.106660628046419No Hit
TATTCATCTCCGGGGGTAGAGCACTGTTTCGGCTGTAGGCACCATCAATT2177271.0504719324535263No Hit
ACTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAATT2027280.9781059488370227No Hit
ATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAATTCGTA1877140.9056675944121825No Hit
AAAGGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATTCGTAT1682820.8119136245718No Hit
CCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA1148820.5542735468918693Illumina Single End Adapter 1 (95% over 22bp)
TAGGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATTCGTATG942750.4548505303984173No Hit
AGCTCAGTAGGTAGAGCAACTGCTGTAGGCACCATCAATTCGTATGCCGT875560.4224332329839705No Hit
GCCGACTTAGCTCAGTAGGTAGAGCAACTGCTGTAGGCACCATCAATTCG840320.40543091774531737No Hit
CCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAATTCGTATGCC832090.40146017272789075No Hit
CACTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAAT743140.3585442833840146No Hit
ACTTGCCCCGAGATGAGTTCTCCCTGACCCCTGTAGGCACCATCAATTCG731400.3528800614515008No Hit
GGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAATTCGTATGCCG634390.30607544733964664No Hit
TTTGCCCCGAGATGAGTTCTCCCTGACCCCTGTAGGCACCATCAATTCGT632150.3049947099351466No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGCTGTAGGCACCATCAATTCG619870.29906995309261936No Hit
ACCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA591400.2853339736702455Illumina Single End Adapter 1 (95% over 22bp)
TCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAATTCGTATGC588660.2840120002379552No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAT562040.2711685941184051Illumina Single End Adapter 1 (95% over 22bp)
CGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAATTCGTATGCCG559870.27012162975779563No Hit
CGGCACGTAGCGCAGCCTGGTAGCGCACCGCTGTAGGCACCATCAATTCG475680.2295023073984822No Hit
TATTCATCTCCGGGGGTAGAGCACTGTTTCGCTGTAGGCACCATCAATTC468120.22585481865829443No Hit
CATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAATTCGT452760.21844404788457955No Hit
GACTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAAT452330.21823658490068001No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTAGGCACCATCAATTC450780.21748875321453037No Hit
GCCGACTCAGCTCAGTAGGTAGAGCAACTGCTGTAGGCACCATCAATTCG445520.21495095020217747No Hit
TGCCGACTTAGCTCAGTAGGTAGAGCAACTGCTGTAGGCACCATCAATTC443100.21378336782767296No Hit
AGGCACGCTGATATGTAGGTGAAGCGACCTGTAGGCACCATCAATTCGTA421680.2034488163971409No Hit
ATGTACACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAATTCGTA384270.18539953679787832No Hit
ATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCACTGTAGGCACCATCAAT370550.1787800201953153No Hit
GGCTACGTAGCTCAGTTGGTTAGAGCACATCCTGTAGGCACCATCAATTC369010.17803701322972149No Hit
TCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAATTCG353170.17039465586932806No Hit
TCCTCTGCAGTTCAGTCGGTAGAACGGCGGCTGTAGGCACCATCAATTCG342950.1654637914612964No Hit
TACTCATCTCCGGGGGTAGAGCACTGTTTCGGCTGTAGGCACCATCAATT330660.15953420989821335No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAG325370.15698193272419306Illumina Single End Adapter 1 (95% over 22bp)
GCCGATACAGCTCAGTTGGTAGAGCAGCGCACTGTAGGCACCATCAATTC323200.15593496836358361No Hit
TAAGGCTGAGGCGTGATGACGAGGCACTGTAGGCACCATCAATTCGTATG322170.1554380221463358No Hit
ACGGTGCTGAAGCAACAAATGCCCTGCTTCCCTGTAGGCACCATCAATTC317380.15312698100010572No Hit
ACCATGCACCGAAGCTGCGGCAGCGACGCCTGTAGGCACCATCAATTCGT305340.14731801745091777No Hit
TTATTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAA300740.14509864599524794No Hit
CAAGGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATTCGTAT291220.14050551202612258No Hit
GCCGATATAGCTCAGTTGGTAGAGCAGCGCACTGTAGGCACCATCAATTC283590.13682425024204417No Hit
CATTCATCTCCGGGGGTAGAGCACTGTTTCGGCTGTAGGCACCATCAATT283360.1367132816692607No Hit
GCCGACTTAGCTCAGTAGGTAGAGCAACTGTAGGCACCATCAATTCGTAT273250.1318354891873429No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAATA268190.12939418058610608Illumina Single End Adapter 1 (95% over 22bp)
ACGGGGCTAAACCATGCACCGAAGCTGCGGCCTGTAGGCACCATCAATTC253150.12213780087017695No Hit
AACTTGCCCCGAGATGAGTTCTCCCTGACCCCTGTAGGCACCATCAATTC246240.11880391896611643No Hit
TTGCCCCGAGATGAGTTCTCCCTGACCCCTGTAGGCACCATCAATTCGTA243120.11729860615270558No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAC241060.11630471371820998Illumina Single End Adapter 1 (95% over 22bp)
TGGGGCTGATTCTGGATTCGACGGGATTTGCGCTGTAGGCACCATCAATT230260.11109401551794172No Hit
AACTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAAT227150.1095935274250867No Hit
GGGGGCTGATTCTGGATTCGACGGGATTTGCGCTGTAGGCACCATCAATT225010.10856103722614463No Hit
TCCTCTGCAGTTCAGTCGGTAGAACGGCGGACTGTAGGCACCATCAATTC222140.1071763424266289No Hit
ACGATGTCGACTTGGAGGTTGTGCCCTGTAGGCACCATCAATTCGTATGC216540.10447449891537869No Hit
ATTCATCTCCGGGGGTAGAGCACTGTTTCGGCTGTAGGCACCATCAATTC216360.10438765394537423No Hit
TGCCGACTCAGCTCAGTAGGTAGAGCAACTGCTGTAGGCACCATCAATTC215970.10419948984369788No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)