FastQCFastQC Report
Mon 3 Jul 2023
SRR4421281.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4421281.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16286018
Total Bases830.5 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA14170108.700776334644846Illumina Single End Adapter 1 (95% over 22bp)
ACCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA4109142.523109086579666Illumina Single End Adapter 1 (95% over 22bp)
AAGGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATTCGTATG3100351.903688182095832No Hit
CCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA3064691.8817920992105008Illumina Single End Adapter 1 (95% over 22bp)
AGAACTCTGAACCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTG685720.42104828816964346Illumina Single End Adapter 1 (95% over 22bp)
TAAGGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATTCGTAT628350.3858217521311839No Hit
CTCTGAACCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAA618170.37957099150940393Illumina Single End Adapter 1 (95% over 22bp)
TAGGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATTCGTATG543480.33370956608300445No Hit
ACGATGTCGACTTGGAGGTTGTGCCCTGTAGGCACCATCAATTCGTATGC444330.2728291225025049No Hit
AACTCTGAACCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAA356320.21878890223503375Illumina Single End Adapter 1 (95% over 22bp)
AGCGCGGTGGTCCCACCTGACCCCCTGTAGGCACCATCAATTCGTATGCC340100.20882943884748253No Hit
CGGCACGTAGCGCAGCCTGGTAGCGCACCGCTGTAGGCACCATCAATTCG338710.2079759459924458No Hit
GACTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA336970.20690754486455804Illumina Single End Adapter 1 (95% over 22bp)
AAAGGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATTCGTAT309470.19002189485483806No Hit
AAGGCTGAGGCGTGATGACGAGGCACTGTAGGCACCATCAATTCGTATGC280480.17222134962640961No Hit
AGGGGTTCGAATCCCCTAGGGGACGCCACTGTAGGCACCATCAATTCGTA279800.17180381355344196No Hit
TTGAGGCGAACCGGGGGAACTGACTGTAGGCACCATCAATTCGTATGCCG277380.1703178763525866No Hit
ATGACGAGGCACCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTG247780.15214277670576073Illumina Single End Adapter 1 (95% over 22bp)
GCGAAGGTGGCGGAATTGGTAGACGCGCCTGTAGGCACCATCAATTCGTA240140.14745163612124218No Hit
TTGCCTGGCGGCAGTAGCGCGGTGGTCCCCTGTAGGCACCATCAATTCGT238280.14630955215694838No Hit
TCGGCACGTAGCGCAGCCTGGTAGCGCACCGCTGTAGGCACCATCAATTC228530.14032282169895674No Hit
GCGAAGGTGGCGGAATTGGTAGACGCGCTGTAGGCACCATCAATTCGTAT227630.13977020042591135No Hit
AAATTGAAGAGTTTGATCATGGCTCCTGTAGGCACCATCAATTCGTATGC217360.13346417767682683No Hit
AGGCACGCTGATATGTAGGTGAAGCGACCTGTAGGCACCATCAATTCGTA199100.12225210607037275No Hit
AAGTCGAACGGTAACAGGAAGAAGCCTGTAGGCACCATCAATTCGTATGC198260.12173632621553039No Hit
TAGGCGTGTGACTGCGTACCTTTTGTCTGTAGGCACCATCAATTCGTATG189360.1162715158487483No Hit
ACGTGGGGGTTCAAGTCCCCCCCCTCGCACCACTGTAGGCACCATCAATT188300.11562065079382819No Hit
TGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA187950.11540574252097718Illumina Single End Adapter 1 (95% over 22bp)
TTTGCCCCGAGATGAGTTCTCCCTGACCCCTGTAGGCACCATCAATTCGT183780.11284526395586693No Hit
ATCACGGGAGACACACGGCGGGTGCTGTAGGCACCATCAATTCGTATGCC180860.11105231493665302No Hit
TTGCCTGGCGGCCGTAGCGCGGTGGTCCCCTGTAGGCACCATCAATTCGT179560.1102540842089208No Hit
CGGACGTGGGGGTTCAAGTCCCCCCCCTCGCCTGTAGGCACCATCAATTC176040.10809272100767665No Hit
TAGGCGTGTGACTGCGTACCTTTTGCTGTAGGCACCATCAATTCGTATGC174090.10689537491607833No Hit
AGGTAACATCAAATCGTACCCCAAACCGCTGTAGGCACCATCAATTCGTA173590.10658836309771977No Hit
AGACCGCCAGCTAAGGTCCCAAAGTCATGGCTGTAGGCACCATCAATTCG171700.10542785842432448No Hit
CGGACTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAA171430.10526207204241086Illumina Single End Adapter 1 (95% over 22bp)
TCTGAACCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAA168140.10324193427761162Illumina Single End Adapter 1 (95% over 22bp)
GGGTCGTTAGCTCAGTTGGTAGAGCCTGTAGGCACCATCAATTCGTATGC166600.10229633787706731No Hit
GATCGTCGGACTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAA166010.10193406393140424Illumina Single End Adapter 1 (95% over 22bp)
ATCAGGTAACATCAAATCGTACCCCCTGTAGGCACCATCAATTCGTATGC164130.10077969949437608No Hit
ACTAATGAACCGTGAGGCTTAACCCTGTAGGCACCATCAATTCGTATGCC163840.10060163263972814No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)