FastQCFastQC Report
Sun 2 Jul 2023
SRR4421283_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4421283_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34259731
Total Bases1.7 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATATCTCGT8937032.6086106747306337No Hit
TAGGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATATCTCGT2060610.6014670693123656No Hit
TATTCATCTCCGGGGGTAGAGCCTGTAGGCACCATCAATATCTCGTATGC1720980.5023331911158322No Hit
ACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCACTGTAGGCACCATC1657380.4837691224137165No Hit
TAAGGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATATCTCG1235500.360627466689683No Hit
TACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCACTGTAGGCACCAT1190660.34753921447894615No Hit
ACTTGCCCCGAGATGAGTTCTCCCTGACCCCTGTAGGCACCATCAATATC1057060.3085429946895964No Hit
ACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCCTGTAGGCACCATCAATA966520.28211546669762233No Hit
AAGTCCCCCCCCTCGCACCACTGTAGGCACCATCAATATCTCGTATGCCG872880.2547830863003565No Hit
CGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCACTGTAGGCACCATCA807950.23583080672758347No Hit
AAGGCTGAGGCGTGATGACGAGGCACTGTAGGCACCATCAATATCTCGTA797410.23275430854959134No Hit
AAGGCTGAGGCGTGATGACGAGGCCTGTAGGCACCATCAATATCTCGTAT759090.22156916526869402No Hit
GCGAAGGTGGCGGAATTGGTAGACGCGCTAGCCTGTAGGCACCATCAATA758070.22127143963856574No Hit
CGGACGTGGGGGTTCAAGTCCCCCCCCTCGCCTGTAGGCACCATCAATAT757610.2211371712171354No Hit
AAAGGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATATCTCG742540.21673842097592652No Hit
TACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCCTGTAGGCACCATCAAT696890.2034137395883231No Hit
ACGCTTTAGCAGCTTAATAACCTGCTTCTGTAGGCACCATCAATATCTCG667550.19484974940404523No Hit
AGCTCAGTAGGTAGAGCAACTGCTGTAGGCACCATCAATATCTCGTATGC635950.1856260926275224No Hit
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA631410.1843009216855789Illumina PCR Primer Index 6 (96% over 29bp)
CGGCACGTAGCGCAGCCTGGTAGCGCACCGCTGTAGGCACCATCAATATC599250.1749138077003582No Hit
AGGCACGCTGATATGTAGGTGAAGCGACCTGTAGGCACCATCAATATCTC583950.17044792324843416No Hit
TGCGCTGTGCGGGTTCAAGTCCCGCTCCGGGCTGTAGGCACCATCAATAT520920.15205023063374315No Hit
TTTGCCCCGAGATGAGTTCTCCCTGACCCCTGTAGGCACCATCAATATCT467590.13648385038399746No Hit
ACGATGTCGACTTGGAGGTTGTGCCCTGTAGGCACCATCAATATCTCGTA437290.1276396478419518No Hit
CAGGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATATCTCGT425190.12410780458258708No Hit
TATTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAAT424420.12388305092062749No Hit
TGGGGCTGATTCTGGATTCGACGGGATTTGCGCTGTAGGCACCATCAATA398110.11620348099055419No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGCTGTAGGCACCATCAATATC372990.10887125762896388No Hit
ATGTACACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAATATCTC364860.10649820922411797No Hit
ATGTACACGGAGTCGACCCAACGCGACTGTAGGCACCATCAATATCTCGT354440.10345673759084681No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)