FastQCFastQC Report
Tue 22 Aug 2023
SRR4421284_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4421284_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences74344118
Total Bases3.7 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATATCTCGT3356700.45150848383190184No Hit
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA3261440.43869509622805664Illumina PCR Primer Index 6 (96% over 29bp)
ACGATGTCGACTTGGAGGTTGTGCCCTGTAGGCACCATCAATATCTCGTA2261360.30417470283257647No Hit
ATCACGGGAGACACACGGCGGGTGCTGTAGGCACCATCAATATCTCGTAT2200020.29592388196736696No Hit
ACTTGCCCCGAGATGAGTTCTCCCTGACCCCTGTAGGCACCATCAATATC2198040.2956575528947697No Hit
ATGTACACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAATATCTC1931920.2598618494606392No Hit
ACTAACTGGAGGACCGAACCGACCTGTAGGCACCATCAATATCTCGTATG1700140.22868520681084684No Hit
TTGAGGCGAACCGGGGGAACTGACTGTAGGCACCATCAATATCTCGTATG1646030.2214068905895151No Hit
TAGGCGTGTGACTGCGTACCTTTTGCTGTAGGCACCATCAATATCTCGTA1558040.20957138801485278No Hit
GCGAAGGTGGCGGAATTGGTAGACGCGCTAGCCTGTAGGCACCATCAATA1400460.18837536010582573No Hit
TTGAGGCGAACCGGGGGAACTGCTGTAGGCACCATCAATATCTCGTATGC1331100.17904577198696472No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTAGGCACCATCAATAT1315010.17688151199803057No Hit
ACGCTTTAGCAGCTTAATAACCTGCTTCTGTAGGCACCATCAATATCTCG1230640.16553293429347027No Hit
AGCGGATGACTTGTGGCTGGGGGCTGTAGGCACCATCAATATCTCGTATG1025570.13794904393108812No Hit
AGGCACGCTGATATGTAGGTGAAGCGACCTGTAGGCACCATCAATATCTC1016410.13671693569624432No Hit
TTTGCCCCGAGATGAGTTCTCCCTGACCCCTGTAGGCACCATCAATATCT996560.13404691948864064No Hit
AGCGGATGACTTGTGGCTGGGGCTGTAGGCACCATCAATATCTCGTATGC968150.1302255008257681No Hit
AGCTCAGTAGGTAGAGCAACTGCTGTAGGCACCATCAATATCTCGTATGC896300.12056098372167116No Hit
ACGTGGGGGTTCAAGTCCCCCCCCTCGCACCACTGTAGGCACCATCAATA879730.11833215910907707No Hit
CGGCACGTAGCGCAGCCTGGTAGCGCACCGCTGTAGGCACCATCAATATC874470.11762463844147025No Hit
AGGCGTGTGACTGCGTACCTTTTGCTGTAGGCACCATCAATATCTCGTAT860220.11570787617656586No Hit
TAGGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATATCTCGT813360.10940475479176442No Hit
ATGAGGCGAACCGGGGGAACTGACTGTAGGCACCATCAATATCTCGTATG760330.10227170897366755No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)