FastQCFastQC Report
Mon 3 Jul 2023
SRR4421285.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4421285.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21830950
Total Bases1.1 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAAT21606239.897063572588458No Hit
AAGGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATTCGTATG14154946.483886408974415No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA5702682.61219965232846Illumina Single End Adapter 1 (95% over 22bp)
TACTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAAT4067451.8631575813237626No Hit
CATTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAAT2611001.1960084192396576No Hit
ACTTGCCCCGAGATGAGTTCTCCCTGACCCCTGTAGGCACCATCAATTCG2375061.087932499501854No Hit
TATTCATCTCCGGGGGTAGAGCACTGTTTCGGCTGTAGGCACCATCAATT2270061.0398356461812244No Hit
ATTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAATT2106470.9649007487076833No Hit
ATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAATTCGTA2044960.9367251539671887No Hit
AATTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAAT1908650.8742862770516171No Hit
GATTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAAT1838770.8422766760035637No Hit
AGCTCAGTAGGTAGAGCAACTGCTGTAGGCACCATCAATTCGTATGCCGT1707130.7819769638975858No Hit
TAAGGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATTCGTAT1618280.7412778646829387No Hit
ACCGGAGTGGACGCATCACTGGTGTTCGGGTCTGTAGGCACCATCAATTC1602470.734035852768661No Hit
ACTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAATT1543320.7069412920647062No Hit
TTTGCCCCGAGATGAGTTCTCCCTGACCCCTGTAGGCACCATCAATTCGT1491910.6833921565483866No Hit
AAAGGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATTCGTAT1027140.4704971611404909No Hit
GCCGACTTAGCTCAGTAGGTAGAGCAACTGCTGTAGGCACCATCAATTCG1011860.46349792381916494No Hit
ACCGCCAGCTAAGGTCCCAAAGTCATGGCTGTAGGCACCATCAATTCGTA1009070.4622199217166454No Hit
ACGGGGCTAAACCATGCACCGAAGCTGCGGCCTGTAGGCACCATCAATTC960770.4400953691891558No Hit
CCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA889830.40760021895519893Illumina Single End Adapter 1 (95% over 22bp)
ACGGTGCTGAAGCAACAAATGCCCTGCTTCCCTGTAGGCACCATCAATTC885410.4055755704630353No Hit
AGACCGCCAGCTAAGGTCCCAAAGTCATGGCTGTAGGCACCATCAATTCG874790.40071091729860586No Hit
TGATCGTCGGACTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGA807290.3697915115924868Illumina Single End Adapter 1 (95% over 22bp)
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGCTGTAGGCACCATCAATTCG779570.3570939423158406No Hit
AAGGCTGAGGCGTGATGACGAGGCACTGTAGGCACCATCAATTCGTATGC756460.34650805393260486No Hit
CCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAATTCGTATGCC752080.34450172805123No Hit
ACTTGCCCCGAGATGAGTTCTCCCTGACTCCCTGTAGGCACCATCAATTC744670.34110746440260276No Hit
ACGGGGCTAAACCATGCACCGAAGCTGCGGCAGCTGTAGGCACCATCAAT703940.32245046596689564No Hit
GATCGTCGGACTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAA700830.32102588297806556Illumina Single End Adapter 1 (95% over 22bp)
GCCGACTCAGCTCAGTAGGTAGAGCAACTGCTGTAGGCACCATCAATTCG627170.28728479521046957No Hit
TTGCCCCGAGATGAGTTCTCCCTGACCCCTGTAGGCACCATCAATTCGTA617040.2826445940282031No Hit
AGGCACGCTGATATGTAGGTGAAGCGACCTGTAGGCACCATCAATTCGTA597420.27365735343629116No Hit
ACCATGCACCGAAGCTGCGGCAGCGACGCCTGTAGGCACCATCAATTCGT597400.27364819213089675No Hit
AACTTGCCCCGAGATGAGTTCTCCCTGACCCCTGTAGGCACCATCAATTC594870.2724892869985044No Hit
TCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAATTCGTATGC568970.26062539651274913No Hit
TCCTCTGCAGTTCAGTCGGTAGAACGGCGGCTGTAGGCACCATCAATTCG560860.2569104871753176No Hit
TATTCATCTCCGGGGGTAGAGCACTGTTTCGCTGTAGGCACCATCAATTC553300.2534475137362323No Hit
ACGGGGCTAAACCATGCACCGAAGCTGCGGCACTGTAGGCACCATCAATT549970.25192215638806376No Hit
ACGATGTCGACTTGGAGGTTGTGCCCTGTAGGCACCATCAATTCGTATGC543250.24884395777554344No Hit
TGCCGACTTAGCTCAGTAGGTAGAGCAACTGCTGTAGGCACCATCAATTC528140.24192259155006995No Hit
TTTGCCCCGAGATGAGTTCTCCCTGACTCCCTGTAGGCACCATCAATTCG522100.23915587732095947No Hit
CACTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAAT505320.23146954209505313No Hit
GGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAATTCGTATGCCG494980.22673314720614543No Hit
CGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAATTCGTATGCCG472990.2166602919249964No Hit
ACCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA464950.21297744715644534Illumina Single End Adapter 1 (95% over 22bp)
CGGCACGTAGCGCAGCCTGGTAGCGCACCGCTGTAGGCACCATCAATTCG456790.20923963455552783No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAT452250.20716001823099772Illumina Single End Adapter 1 (95% over 22bp)
GCCGACTTAGCTCAGTAGGTAGAGCAACTGTAGGCACCATCAATTCGTAT435470.19947368300509138No Hit
TACTCATCTCCGGGGGTAGAGCACTGTTTCGGCTGTAGGCACCATCAATT380050.17408770575719335No Hit
TGGGCGTTAAGTTGCAGGGTATAGACCCGCTGTAGGCACCATCAATTCGT367790.16847182555042267No Hit
TAGGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATTCGTATG356960.16351097867935202No Hit
AACGATGTCGACTTGGAGGTTGTGCCCTGTAGGCACCATCAATTCGTATG351220.1608816840311576No Hit
CATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAATTCGT339020.15529328774057016No Hit
GCCGATACAGCTCAGTTGGTAGAGCAGCGCACTGTAGGCACCATCAATTC338280.15495431944097715No Hit
TCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAATTCG319020.1461319823461645No Hit
AACGGGGCTAAACCATGCACCGAAGCTGCGGCCTGTAGGCACCATCAATT310450.1422063629846617No Hit
CCGAGATGAGTTCTCCCTGACCCCTGTAGGCACCATCAATTCGTATGCCG307170.14070390889997916No Hit
TTATTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAA306730.14050236018130224No Hit
GACTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAAT305810.14008094013315958No Hit
TGCCGACTCAGCTCAGTAGGTAGAGCAACTGCTGTAGGCACCATCAATTC294590.13494144780689799No Hit
TCCCCATGCGAGAGTAGGGAACTGCCAGGCACTGTAGGCACCATCAATTC284570.13035163380430076No Hit
TAGGCACGCTGATATGTAGGTGAAGCGACCTGTAGGCACCATCAATTCGT281660.12901866386941474No Hit
GAGATGAGTTCTCCCTGACCCCTGTAGGCACCATCAATTCGTATGCCGTC276150.126494724233256No Hit
CATTCATCTCCGGGGGTAGAGCACTGTTTCGGCTGTAGGCACCATCAATT271770.12448839835188115No Hit
TACGGTGCTGAAGCAACAAATGCCCTGCTTCCCTGTAGGCACCATCAATT271570.1243967852979371No Hit
GCCGATATAGCTCAGTTGGTAGAGCAGCGCACTGTAGGCACCATCAATTC269970.12366388086638466No Hit
AGCTCAGTTGGTAGAGCAGCGCACTGTAGGCACCATCAATTCGTATGCCG268100.12280729881200772No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAG265340.12154303866757975Illumina Single End Adapter 1 (95% over 22bp)
TGCCGACTTAGCTCAGTAGGTAGAGCAACTGTAGGCACCATCAATTCGTA260190.1191840025285203No Hit
AGCGCACGCAGGCGGTTTGTTAAGTCAGATGCTGTAGGCACCATCAATTC254760.11669670811393915No Hit
TTGCCCCGAGATGAGTTCTCCCTGACTCCCTGTAGGCACCATCAATTCGT252500.11566148060437131No Hit
AGTTCAGTCGGTAGAACGGCGGCTGTAGGCACCATCAATTCGTATGCCGT241730.11072811764948388No Hit
CAGTTCAGTCGGTAGAACGGCGGCTGTAGGCACCATCAATTCGTATGCCG239040.10949592207393631No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTAGGCACCATCAATTC237750.10890501787599716No Hit
ATTCATCTCCGGGGGTAGAGCACTGTTTCGGCTGTAGGCACCATCAATTC231130.10587262579044888No Hit
GGGGGCTGATTCTGGATTCGACGGGATTTGCGCTGTAGGCACCATCAATT222530.10193326447085445No Hit
AACGGGGCTAAACCATGCACCGAAGCTGCGGCACTGTAGGCACCATCAAT222170.10176836097375516No Hit
GCGAAGGTGGCGGAATTGGTAGACGCGCTGTAGGCACCATCAATTCGTAT221530.10147519920113417No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAATA220470.10098965001523066Illumina Single End Adapter 1 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)