FastQCFastQC Report
Mon 3 Jul 2023
SRR4421286_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4421286_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34735497
Total Bases1.7 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATATCTCGT5098111.4676945604089098No Hit
ACTTGCCCCGAGATGAGTTCTCCCTGACCCCTGTAGGCACCATCAATATC2318070.6673490233924104No Hit
ACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCACTGTAGGCACCATC2157100.6210073804327602No Hit
ACCGGAGTGGACGCATCACTGGTGTTCGGGTCTGTAGGCACCATCAATAT1828040.5262743181708326No Hit
AGACCGCCAGCTAAGGTCCCAAAGTCATGGCTGTAGGCACCATCAATATC1705100.49088112946822093No Hit
TACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCACTGTAGGCACCAT1456710.4193721483242345No Hit
GCGAAGGTGGCGGAATTGGTAGACGCGCTAGCCTGTAGGCACCATCAATA1343570.3868002809920929No Hit
CGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCACTGTAGGCACCATCA1320310.3801039610862629No Hit
TTGCCGTGGGCGCTGGAGAACTGAGGGGGGCTGCTCCCTGTAGGCACCAT1262910.3635790787735094No Hit
TAACCGGGAGATAGCTGGTTCTCCCCGAAAGCTCTGTAGGCACCATCAAT966990.27838668898274294No Hit
ACCGCCAGCTAAGGTCCCAAAGTCATGGCTGTAGGCACCATCAATATCTC926230.2666522952010734No Hit
ACGATGTCGACTTGGAGGTTGTGCCCTGTAGGCACCATCAATATCTCGTA893930.2573534502759526No Hit
TAGGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATATCTCGT853680.24576588036152183No Hit
TGGGCGTTAAGTTGCAGGGTATAGACCCGCTGTAGGCACCATCAATATCT814410.23446044258413806No Hit
AAAACCGGGAGATAGCTGGTTCTCCCCGAAAGCTCTGTAGGCACCATCAA775220.2231780360016153No Hit
CGGCACGTAGCGCAGCCTGGTAGCGCACCGCTGTAGGCACCATCAATATC771460.2220955698431492No Hit
TATTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAAT766540.22067915135919894No Hit
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA753690.21697976568465396Illumina PCR Primer Index 6 (96% over 29bp)
AGCTCAGTAGGTAGAGCAACTGCTGTAGGCACCATCAATATCTCGTATGC699930.2015028027380751No Hit
TATTCATCTCCGGGGGTAGAGCCTGTAGGCACCATCAATATCTCGTATGC687100.1978091748622454No Hit
CGGTGGTTCAAGTCCACTCAGGCCCTGTAGGCACCATCAATATCTCGTAT675680.19452147179584042No Hit
AACTTGCCCCGAGATGAGTTCTCCCTGACCCCTGTAGGCACCATCAATAT675330.19442071031832364No Hit
TAAGGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATATCTCG664060.19117619074228304No Hit
AGGCACGCTGATATGTAGGTGAAGCGACCTGTAGGCACCATCAATATCTC650780.18735301239536029No Hit
TTTGCCCCGAGATGAGTTCTCCCTGACCCCTGTAGGCACCATCAATATCT625380.180040608026999No Hit
AGGCTTGTAGCTCAGGTGGTTAGAGCGCACCCCCTGTAGGCACCATCAAT617110.17765975825824518No Hit
ACTTGCCCCGAGATGAGTTCTCCCTGACTCCCTGTAGGCACCATCAATAT604240.17395461478498495No Hit
CGGAGGTTCGAATCCTCCCGGATGCCTGTAGGCACCATCAATATCTCGTA597080.17189332284492717No Hit
ACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCCTGTAGGCACCATCAATA588020.16928504002692119No Hit
GCCGACTTAGCTCAGTAGGTAGAGCAACTGCTGTAGGCACCATCAATATC577290.16619598101619218No Hit
TGGGGCTGATTCTGGATTCGACGGGATTTGCGCTGTAGGCACCATCAATA575180.1655885332517338No Hit
ACTAACTGGAGGACCGAACCGACCTGTAGGCACCATCAATATCTCGTATG562990.1620791549347919No Hit
GCCGACTCAGCTCAGTAGGTAGAGCAACTGCTGTAGGCACCATCAATATC545670.15709290124738967No Hit
AACGATGTCGACTTGGAGGTTGTGCCCTGTAGGCACCATCAATATCTCGT541870.15599891949149308No Hit
AAAACCGGGAGATAGCTGGTTCTCCCCGAAAGCTGTAGGCACCATCAATA537780.15482144965422548No Hit
ATGGCCAGGCTGTCTCCACCCGAGACTCAGTGCTGTAGGCACCATCAATA521230.15005687121736017No Hit
GCCGAGGTGGTGGAATTGGTAGACACGCTACCCTGTAGGCACCATCAATA511090.1471376672687309No Hit
CGGACGTGGGGGTTCAAGTCCCCCCCCTCGCCTGTAGGCACCATCAATAT506650.14585943595394646No Hit
ATCACGGGAGACACACGGCGGGTGCTGTAGGCACCATCAATATCTCGTAT469370.13512689914872963No Hit
TTTCGCCATTTAAAGTGGTACGCGAGCTGGGCTGTAGGCACCATCAATAT467870.13469506424508623No Hit
TCAAACCGGGAGATAGCTGGTTCTCCCCGAAAGCTCTGTAGGCACCATCA464340.13367881277184548No Hit
AGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCCTGTAGGCACCATCA461100.13274604937997578No Hit
AAGGCTGAGGCGTGATGACGAGGCACTGTAGGCACCATCAATATCTCGTA450450.12968002156410777No Hit
GGGGCTGATTCTGGATTCGACGGGATTTGCGCTGTAGGCACCATCAATAT430080.12381570357263062No Hit
AGGTAACATCAAATCGTACCCCAAACCGCTGTAGGCACCATCAATATCTC419440.12075255465612023No Hit
GCCGACTTAGCTCAGTAGGTAGAGCAACTGTAGGCACCATCAATATCTCG419290.12070937116575589No Hit
AAAGGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATATCTCG410950.11830836910149867No Hit
AAACCGGGAGATAGCTGGTTCTCCCCGAAAGCTCTGTAGGCACCATCAAT408730.11766925344410648No Hit
AACTCGCTGTGAAGATGCAGTGTACCCGCGGCACTGTAGGCACCATCAAT400940.11542659084451849No Hit
TACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCCTGTAGGCACCATCAAT400230.1152221889901273No Hit
AGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCTGTAGGCACCAT398280.1146608036153909No Hit
CCGAGATGAGTTCTCCCTGACCCCTGTAGGCACCATCAATATCTCGTATG397850.11453701094301313No Hit
GCCGATACAGCTCAGTTGGTAGAGCAGCGCACTGTAGGCACCATCAATAT394540.11358409525564006No Hit
ACGGTGCTGAAGCAACAAATGCCCTGCTTCCCTGTAGGCACCATCAATAT391720.11277224563679052No Hit
GCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTCTGTAGGCACCATCAAT388600.11187402903721229No Hit
AGCGCACGCAGGCGGTTTGTTAAGTCAGATGCTGTAGGCACCATCAATAT383200.1103194233840961No Hit
AGGGCTTACGGGTTCAAGTCCCGTCCTCGGTACCACTGTAGGCACCATCA380110.10942984348259073No Hit
AACGATGTCGACTTGGAGGTTGTGCCCCTGTAGGCACCATCAATATCTCG376260.10832146722990604No Hit
GCGAAGGTGGCGGAATTGGTAGACGCGCCTGTAGGCACCATCAATATCTC375410.10807676078450815No Hit
TTGGGCGTTAAGTTGCAGGGTATAGACCCGCTGTAGGCACCATCAATATC372670.10728794236051956No Hit
ATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCACTGTAGGCACCATCAAT368330.10603850003931138No Hit
GCGGGAATAGCTCAGTTGGTAGAGCACGACCCTGTAGGCACCATCAATAT366450.10549726696007834No Hit
TAGGCACGCTGATATGTAGGTGAAGCGACCTGTAGGCACCATCAATATCT359400.10346764291295443No Hit
ACGCTTTAGCAGCTTAATAACCTGCTTCTGTAGGCACCATCAATATCTCG356300.10257518411209143No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)