FastQCFastQC Report
Mon 3 Jul 2023
SRR4421288.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4421288.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33811110
Total Bases1.7 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATTCGTATG415013112.274459489794923No Hit
TATTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAAT26273157.770567130153373No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA6071321.7956582910173609Illumina Single End Adapter 1 (95% over 22bp)
TACTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAAT6003961.7757358454070276No Hit
ATTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAATT5154521.5245048151332505No Hit
TAAGGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATTCGTAT4705111.3915869665325982No Hit
AAGGCTGAGGCGTGATGACGAGGCACTGTAGGCACCATCAATTCGTATGC4008341.1855097333391302No Hit
CATTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAAT3998771.182679302749895No Hit
TATTCATCTCCGGGGGTAGAGCACTGTTTCGGCTGTAGGCACCATCAATT3082140.9115761061970459No Hit
AAAGGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATTCGTAT2931840.8671232621466731No Hit
ATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAATTCGTA2912890.8615185955149062No Hit
ACTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAATT2808020.8305021633421678No Hit
GATTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAAT2562610.7579195122549955No Hit
TAGGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATTCGTATG2553080.7551009120966451No Hit
AATTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAAT2195360.6493013686921252No Hit
ACTTGCCCCGAGATGAGTTCTCCCTGACCCCTGTAGGCACCATCAATTCG1792310.5300949894871834No Hit
AGCTCAGTAGGTAGAGCAACTGCTGTAGGCACCATCAATTCGTATGCCGT1714620.5071173351007997No Hit
TTTGCCCCGAGATGAGTTCTCCCTGACCCCTGTAGGCACCATCAATTCGT1513310.44757773406433565No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGCTGTAGGCACCATCAATTCG1306930.3865386259131983No Hit
GCCGACTTAGCTCAGTAGGTAGAGCAACTGCTGTAGGCACCATCAATTCG1253240.37065923005781237No Hit
AAGGCTGAGGCGTGATGACGAGGCCTGTAGGCACCATCAATTCGTATGCC1024280.30294184367209476No Hit
AGGCACGCTGATATGTAGGTGAAGCGACCTGTAGGCACCATCAATTCGTA991560.29326455120816797No Hit
ACGGTGCTGAAGCAACAAATGCCCTGCTTCCCTGTAGGCACCATCAATTC990020.2928090796190956No Hit
CCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAATTCGTATGCC931740.27557214181965634No Hit
CACTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAAT912020.2697397393933532No Hit
ACGATGTCGACTTGGAGGTTGTGCCCTGTAGGCACCATCAATTCGTATGC883140.2611981682943861No Hit
TCCTCTGCAGTTCAGTCGGTAGAACGGCGGCTGTAGGCACCATCAATTCG848060.2508228804082445No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTAGGCACCATCAATTC828900.24515610401433136No Hit
CCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA773200.22868222900697432Illumina Single End Adapter 1 (95% over 22bp)
CGGCACGTAGCGCAGCCTGGTAGCGCACCGCTGTAGGCACCATCAATTCG707490.209247788670647No Hit
ACCATGCACCGAAGCTGCGGCAGCGACGCCTGTAGGCACCATCAATTCGT692070.204687157564481No Hit
TTGCCCCGAGATGAGTTCTCCCTGACCCCTGTAGGCACCATCAATTCGTA676430.20006145908844755No Hit
TCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAATTCGTATGC672810.1989908050933554No Hit
GGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAATTCGTATGCCG668990.1978609989438383No Hit
GCCGACTCAGCTCAGTAGGTAGAGCAACTGCTGTAGGCACCATCAATTCG667500.1974203153933722No Hit
CGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAATTCGTATGCCG653410.1932530461141323No Hit
ACTTGCCCCGAGATGAGTTCTCCCTGACTCCCTGTAGGCACCATCAATTC643370.19028360796199828No Hit
ATTCATCTCCGGGGGTAGAGCACTGTTTCGGCTGTAGGCACCATCAATTC611930.18098488928639136No Hit
TTTGCCCCGAGATGAGTTCTCCCTGACTCCCTGTAGGCACCATCAATTCG607660.17972199078941803No Hit
TGCCGACTTAGCTCAGTAGGTAGAGCAACTGCTGTAGGCACCATCAATTC603030.17835261841447975No Hit
AACTTGCCCCGAGATGAGTTCTCCCTGACCCCTGTAGGCACCATCAATTC555950.16442820126283936No Hit
GGCTACGTAGCTCAGTTGGTTAGAGCACATCCTGTAGGCACCATCAATTC547480.16192310752294142No Hit
TACTCATCTCCGGGGGTAGAGCACTGTTTCGGCTGTAGGCACCATCAATT521020.1540972774925165No Hit
GACTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAAT519200.1535589928872492No Hit
CAAGGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATTCGTAT514650.15221328137408088No Hit
CATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAATTCGT509160.15058955473511518No Hit
TATTCATCTCCGGGGGTAGAGCACTGTTTCGCTGTAGGCACCATCAATTC507410.15007197338389658No Hit
ACGGGGCTAAACCATGCACCGAAGCTGCGGCCTGTAGGCACCATCAATTC482490.14270161494254402No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAT470960.13929149323994391Illumina Single End Adapter 1 (95% over 22bp)
TAAGGCTGAGGCGTGATGACGAGGCACTGTAGGCACCATCAATTCGTATG468870.13867335322620286No Hit
TCCTCTGCAGTTCAGTCGGTAGAACGGCGGACTGTAGGCACCATCAATTC462220.13670654409157226No Hit
CATTCATCTCCGGGGGTAGAGCACTGTTTCGGCTGTAGGCACCATCAATT458330.1355560346880064No Hit
GCCGATACAGCTCAGTTGGTAGAGCAGCGCACTGTAGGCACCATCAATTC445870.13187085546733013No Hit
GCCGACTTAGCTCAGTAGGTAGAGCAACTGTAGGCACCATCAATTCGTAT425240.12576931073839337No Hit
ACCGGAGTGGACGCATCACTGGTGTTCGGGTCTGTAGGCACCATCAATTC421670.12471344478190748No Hit
AGTTCAGTCGGTAGAACGGCGGCTGTAGGCACCATCAATTCGTATGCCGT416960.12332041154519921No Hit
TAGGCACGCTGATATGTAGGTGAAGCGACCTGTAGGCACCATCAATTCGT408310.1207620808663188No Hit
CAGTTCAGTCGGTAGAACGGCGGCTGTAGGCACCATCAATTCGTATGCCG394090.11655636268670269No Hit
GCCGATATAGCTCAGTTGGTAGAGCAGCGCACTGTAGGCACCATCAATTC389520.11520473595809189No Hit
TCCCCATGCGAGAGTAGGGAACTGCCAGGCACTGTAGGCACCATCAATTC385400.1139862015769373No Hit
TCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAATTCG384440.11370227123569739No Hit
GGGGGCTGATTCTGGATTCGACGGGATTTGCGCTGTAGGCACCATCAATT380590.1125635922630165No Hit
TTATTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAA358410.10600361833728617No Hit
TTGGGGTACCGCCAAGCGGTAAGGCACCGGCTGTAGGCACCATCAATTCG351350.10391554728608438No Hit
ACCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA347200.10268814008176602Illumina Single End Adapter 1 (95% over 22bp)
ATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCACTGTAGGCACCATCAAT344760.10196648379778125No Hit
AGGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATTCGTATGC340740.10077752549383916No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)