FastQCFastQC Report
Mon 3 Jul 2023
SRR4421290_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4421290_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31007897
Total Bases1.5 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATATCTCGT11760913.7928757309791115No Hit
TAGGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATATCTCGT2642160.8520926136977299No Hit
TAAGGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATATCTCG1449620.46750026291689495No Hit
ACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCACTGTAGGCACCATC1102670.35560941137027124No Hit
GCGAAGGTGGCGGAATTGGTAGACGCGCTAGCCTGTAGGCACCATCAATA1097770.35402916876304125No Hit
CGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCACTGTAGGCACCATCA955140.30803120895299674No Hit
AAGGCTGAGGCGTGATGACGAGGCACTGTAGGCACCATCAATATCTCGTA933750.30113296622470076No Hit
AAAGGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATATCTCG907210.2925738562663569No Hit
ACTTGCCCCGAGATGAGTTCTCCCTGACCCCTGTAGGCACCATCAATATC820420.2645842122089092No Hit
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA781480.2520261209587996Illumina PCR Primer Index 6 (96% over 29bp)
CGGACGTGGGGGTTCAAGTCCCCCCCCTCGCCTGTAGGCACCATCAATAT753140.24288651371616718No Hit
AAGGCTGAGGCGTGATGACGAGGCCTGTAGGCACCATCAATATCTCGTAT751540.2423705161301329No Hit
ACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCCTGTAGGCACCATCAATA744920.24023557611791602No Hit
CGGCACGTAGCGCAGCCTGGTAGCGCACCGCTGTAGGCACCATCAATATC696700.22468469886880751No Hit
ACGCTTTAGCAGCTTAATAACCTGCTTCTGTAGGCACCATCAATATCTCG635520.20495424117282124No Hit
TATTCATCTCCGGGGGTAGAGCCTGTAGGCACCATCAATATCTCGTATGC627350.20231942849913365No Hit
AGGCACGCTGATATGTAGGTGAAGCGACCTGTAGGCACCATCAATATCTC559390.18040243103232703No Hit
TACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCACTGTAGGCACCAT548950.17703554678345326No Hit
AGCTCAGTAGGTAGAGCAACTGCTGTAGGCACCATCAATATCTCGTATGC535250.17261731745303463No Hit
CAGGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATATCTCGT490040.15803716066265314No Hit
GCCGAGGTGGTGGAATTGGTAGACACGCTACCCTGTAGGCACCATCAATA488250.15745988836327726No Hit
TACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCCTGTAGGCACCATCAAT454680.14663361401129524No Hit
GCCGATACAGCTCAGTTGGTAGAGCAGCGCACTGTAGGCACCATCAATAT425240.13713925842826427No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTAGGCACCATCAATAT403670.13018296597153944No Hit
ACGATGTCGACTTGGAGGTTGTGCCCTGTAGGCACCATCAATATCTCGTA380460.12269777598912948No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGCTGTAGGCACCATCAATATC374520.12078213495097716No Hit
ACGTGGGGGTTCAAGTCCCCCCCCTCGCACCACTGTAGGCACCATCAATA367010.1183601712815287No Hit
TGCGCTGTGCGGGTTCAAGTCCCGCTCCGGGCTGTAGGCACCATCAATAT359300.11587370791382595No Hit
CGCTGTGCGGGTTCAAGTCCCGCTCCGGGTACCACTGTAGGCACCATCAA348240.1123068746003639No Hit
ATCACGGGAGACACACGGCGGGTGCTGTAGGCACCATCAATATCTCGTAT324720.10472171008565978No Hit
TATTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAAT312140.10066467906546517No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)