FastQCFastQC Report
Mon 3 Jul 2023
SRR4421291.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4421291.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16723193
Total Bases852.8 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA199944111.956095944117848Illumina Single End Adapter 1 (95% over 22bp)
ACCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA5570453.330972739476247Illumina Single End Adapter 1 (95% over 22bp)
CCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA4210802.517940204361691Illumina Single End Adapter 1 (95% over 22bp)
AAGGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATTCGTATG4080342.4399287863268695No Hit
TAAGGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATTCGTAT802890.48010568316708424No Hit
AGAACTCTGAACCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTG790380.4726250543182753Illumina Single End Adapter 1 (95% over 22bp)
TTGTCCCGGTTTAAGCGTGTAGGCTGGCTGTAGGCACCATCAATTCGTAT673130.40251284548351496No Hit
TAGGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATTCGTATG663460.39673045691692965No Hit
CTCTGAACCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAA448180.26799905974893673Illumina Single End Adapter 1 (95% over 22bp)
AAAGGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATTCGTAT428730.25636850570342634No Hit
GACTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA415980.24874436359133092Illumina Single End Adapter 1 (95% over 22bp)
ACGCTTTAGCAGCTTAATAACCTGCTTCTGTAGGCACCATCAATTCGTAT390740.2336515520690337No Hit
TAGGCGTGTGACTGCGTACCTTTTGTCTGTAGGCACCATCAATTCGTATG363990.21765580293189227No Hit
AAGGCTGAGGCGTGATGACGAGGCACTGTAGGCACCATCAATTCGTATGC345520.20661126137813515No Hit
TGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA326500.1952378352626798Illumina Single End Adapter 1 (95% over 22bp)
ACGATGTCGACTTGGAGGTTGTGCCCTGTAGGCACCATCAATTCGTATGC324160.1938385809456364No Hit
ATGACGAGGCACCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTG296640.1773823934221174Illumina Single End Adapter 1 (95% over 22bp)
TTGAGGCGTGGCTTCCGGAGCTAACGCGTTCTGTAGGCACCATCAATTCG294470.1760847943332353No Hit
AACTCTGAACCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAA288430.1724730438738583Illumina Single End Adapter 1 (95% over 22bp)
GTTGTCCCGGTTTAAGCGTGTAGGCTGCTGTAGGCACCATCAATTCGTAT284120.16989578485400486No Hit
TTGTCCCGGTTTAAGCGTGTAGGCTGCTGTAGGCACCATCAATTCGTATG280300.16761153208002802No Hit
TTTGTCCCGGTTTAAGCGTGTAGGCTGGCTGTAGGCACCATCAATTCGTA269640.16123715130238583No Hit
TACGCTTTAGCAGCTTAATAACCTGCTTCTGTAGGCACCATCAATTCGTA268020.16026843677520197No Hit
GCGAAGGTGGCGGAATTGGTAGACGCGCCTGTAGGCACCATCAATTCGTA260260.15562817459560505No Hit
GCGAAGGTGGCGGAATTGGTAGACGCGCTGTAGGCACCATCAATTCGTAT242900.145247381884548No Hit
TAGGCGTGTGACTGCGTACCTTTTGTATCTGTAGGCACCATCAATTCGTA237940.14228144110996027No Hit
ATCAGGTAACATCAAATCGTACCCCCTGTAGGCACCATCAATTCGTATGC237750.14216782644319179No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAT224240.1340892256640224Illumina Single End Adapter 1 (95% over 22bp)
TCGTCGGACTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAA222210.13287534264539075Illumina Single End Adapter 1 (95% over 22bp)
AAATTGAAGAGTTTGATCATGGCTCCTGTAGGCACCATCAATTCGTATGC218600.1307166639767896No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAG207160.1238758650934663Illumina Single End Adapter 1 (95% over 22bp)
AGCGCGGTGGTCCCACCTGACCCCCTGTAGGCACCATCAATTCGTATGCC206610.12354698053176807No Hit
GTTGTCCCGGTTTAAGCGTGTAGGCTGGCTGTAGGCACCATCAATTCGTA204980.12257228628528058No Hit
CGGCACGTAGCGCAGCCTGGTAGCGCACCGCTGTAGGCACCATCAATTCG203320.12157965288088225No Hit
ACGATGTCGACTTGGAGGTTGTGCCCCTGTAGGCACCATCAATTCGTATG202130.12086806628375335No Hit
AACGATGTCGACTTGGAGGTTGTGCCCCTGTAGGCACCATCAATTCGTAT195700.1170231067715358No Hit
ACTAATGAACCGTGAGGCTTAACCCTGTAGGCACCATCAATTCGTATGCC192980.11539662312095543No Hit
AAGTCGAACGGTAACAGGAAGAAGCCTGTAGGCACCATCAATTCGTATGC191000.1142126386988418No Hit
TTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA187370.11204200059163343Illumina Single End Adapter 1 (95% over 22bp)
ACGTGGGGGTTCAAGTCCCCCCCCTCGCACCACTGTAGGCACCATCAATT187010.11182673069670367No Hit
CGGACTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAA182140.10891460739584839Illumina Single End Adapter 1 (95% over 22bp)
ACCTGCGGTTGGATCACCTCCCTGTAGGCACCATCAATTCGTATGCCGTC180560.1079698117458789No Hit
TTGCCTGGCGGCAGTAGCGCGGTGGTCCCCTGTAGGCACCATCAATTCGT175280.10481251995357586No Hit
AGGGGTTCGAATCCCCTAGGGGACGCCACTGTAGGCACCATCAATTCGTA170720.1020857679511323No Hit
TTTGCCCCGAGATGAGTTCTCCCTGACCCCTGTAGGCACCATCAATTCGT169080.1011050939853412No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)