FastQCFastQC Report
Mon 3 Jul 2023
SRR4421292.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4421292.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17423341
Total Bases888.5 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA237854913.651509202511733Illumina Single End Adapter 1 (95% over 22bp)
ACCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA8270814.746971318531847Illumina Single End Adapter 1 (95% over 22bp)
AAGGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATTCGTATG4517692.5928953580142866No Hit
CCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA3952642.268589015160755Illumina Single End Adapter 1 (95% over 22bp)
TCGACACACTATCATTAACTGAATCCTGTAGGCACCATCAATTCGTATGC2515821.4439366135346832No Hit
AGAACTCTGAACCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTG890470.5110787879316602Illumina Single End Adapter 1 (95% over 22bp)
TAAGGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATTCGTAT852980.4895616747671988No Hit
ATGACGAGGCACCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTG713980.40978363449352223Illumina Single End Adapter 1 (95% over 22bp)
TGATGACGAGGCACCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCT709800.4073845538579541No Hit
TTCGACACACTATCATTAACTGAATCCTGTAGGCACCATCAATTCGTATG707040.4058004719071962No Hit
TGAGGCACCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAA693860.3982359066495915Illumina Single End Adapter 1 (95% over 22bp)
TAGGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATTCGTATG625420.358955265812682No Hit
TCGTCGGACTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAA555120.3186070914872182Illumina Single End Adapter 1 (95% over 22bp)
GACTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA526430.30214067439763703Illumina Single End Adapter 1 (95% over 22bp)
GACGAGGCACCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAA487450.2797683865568607Illumina Single End Adapter 1 (95% over 22bp)
ATGTACACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAATTCGTA486590.2792747958040883No Hit
CTCTGAACCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAA480590.27583113938939724Illumina Single End Adapter 1 (95% over 22bp)
TTTCGACACACTATCATTAACTGAATCCTGTAGGCACCATCAATTCGTAT444760.2552667711663337No Hit
AAAGGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATTCGTAT433010.24852294402089703No Hit
GATGACGAGGCACCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTT428710.24605499025703506Illumina Single End Adapter 1 (95% over 21bp)
TGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA419830.24095837876329232Illumina Single End Adapter 1 (95% over 22bp)
GATCGTCGGACTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAA372280.21366740167686554Illumina Single End Adapter 1 (95% over 22bp)
AAGGCTGAGGCGTGATGACGAGGCACTGTAGGCACCATCAATTCGTATGC358140.20555185139291024No Hit
ACGAGGCACCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAA355420.2039907271515836Illumina Single End Adapter 1 (95% over 22bp)
ACGATGTCGACTTGGAGGTTGTGCCCTGTAGGCACCATCAATTCGTATGC351910.20197618814898932No Hit
AACTCTGAACCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAA310900.17843879655457584Illumina Single End Adapter 1 (95% over 22bp)
TGGTATCCTGTCTGAATATGGGGGGACCTGTAGGCACCATCAATTCGTAT308390.17699820028776342No Hit
ATCATTAACTGAATCCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGC290470.16671314646255272No Hit
ATTAACTGAATCCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTG281620.1616337532508834Illumina Single End Adapter 1 (95% over 22bp)
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAT278290.15972252394072986Illumina Single End Adapter 1 (95% over 22bp)
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAG260740.14964982892775844Illumina Single End Adapter 1 (95% over 22bp)
ACGCTTTAGCAGCTTAATAACCTGCTTCTGTAGGCACCATCAATTCGTAT241730.13873917752054557No Hit
ATCACGGGAGACACACGGCGGGTGCTGTAGGCACCATCAATTCGTATGCC240820.13821688963098408No Hit
TTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA237720.13643766715006037Illumina Single End Adapter 1 (95% over 22bp)
AAGGCTGAGGCGTGATGACGAGGCACTCTGTAGGCACCATCAATTCGTAT234340.13449774070311774No Hit
TAGGCGTGTGACTGCGTACCTTTTGTCTGTAGGCACCATCAATTCGTATG232960.1337056997277388No Hit
ACTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA210100.12058536878776578Illumina Single End Adapter 1 (95% over 22bp)
TGACGAGGCACCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGA208920.11990811635954322Illumina Single End Adapter 1 (95% over 22bp)
ACCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTTAAAAAAAAAA208000.11938008904262391Illumina Single End Adapter 1 (95% over 21bp)
ACGATGTCGACTTGGAGGTTGTGCCCCTGTAGGCACCATCAATTCGTATG196890.11300358524808762No Hit
ACTATCATTAACTGAATCCTGTAGGCACCATCAATTCGTATGCCGTCTTC194660.11172369294729409No Hit
TAGGCGTGTGACTGCGTACCTTTTGCTGTAGGCACCATCAATTCGTATGC182690.1048535983999854No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAC179070.10277592569645512Illumina Single End Adapter 1 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)