FastQCFastQC Report
Mon 3 Jul 2023
SRR4421293_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4421293_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52848625
Total Bases2.6 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATATCTCGT10538241.994042418322142No Hit
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA3675840.6955412747256906Illumina PCR Primer Index 6 (96% over 29bp)
ACGCTTTAGCAGCTTAATAACCTGCTTCTGTAGGCACCATCAATATCTCG2269500.4294340675845398No Hit
ACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCACTGTAGGCACCATC2195740.41547722386344016No Hit
AGCTCAGTAGGTAGAGCAACTGCTGTAGGCACCATCAATATCTCGTATGC1928860.3649782752152965No Hit
GCGAAGGTGGCGGAATTGGTAGACGCGCTAGCCTGTAGGCACCATCAATA1795640.33977042922119544No Hit
CGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCACTGTAGGCACCATCA1752070.33152612769017165No Hit
ACTTGCCCCGAGATGAGTTCTCCCTGACCCCTGTAGGCACCATCAATATC1559840.2951524282798275No Hit
ACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCCTGTAGGCACCATCAATA1528700.2892601273921507No Hit
CGGCACGTAGCGCAGCCTGGTAGCGCACCGCTGTAGGCACCATCAATATC1495270.28293451343341475No Hit
TAGGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATATCTCGT1477600.27959100165803746No Hit
CGGACGTGGGGGTTCAAGTCCCCCCCCTCGCCTGTAGGCACCATCAATAT1149650.21753640704937927No Hit
TAAGGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATATCTCG1077140.20381608793038608No Hit
TACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCACTGTAGGCACCAT1046140.19795027779814517No Hit
AGGCACGCTGATATGTAGGTGAAGCGACCTGTAGGCACCATCAATATCTC967540.1830776108176892No Hit
GCCGATACAGCTCAGTTGGTAGAGCAGCGCACTGTAGGCACCATCAATAT954560.18062153934941544No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGCTGTAGGCACCATCAATATC901900.1706572309118733No Hit
CGCTGTGCGGGTTCAAGTCCCGCTCCGGGCTGTAGGCACCATCAATATCT806490.15260378108228928No Hit
GCCGACTTAGCTCAGTAGGTAGAGCAACTGCTGTAGGCACCATCAATATC796680.15074753600495755No Hit
TACGCTTTAGCAGCTTAATAACCTGCTTCTGTAGGCACCATCAATATCTC719840.1362078956642675No Hit
TACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCCTGTAGGCACCATCAAT718710.13599407742396324No Hit
AAGGCTGAGGCGTGATGACGAGGCACTGTAGGCACCATCAATATCTCGTA718510.1359562334876262No Hit
GCCGAGGTGGTGGAATTGGTAGACACGCTACCCTGTAGGCACCATCAATA708210.13400727076626875No Hit
TCGCGCTGTGCGGGTTCAAGTCCCGCTCCGGGCTGTAGGCACCATCAATA704960.1333923068007919No Hit
AAAGGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATATCTCG685600.1297290137633666No Hit
TATTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAAT677270.12815281381492896No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTAGGCACCATCAATAT661380.1251461130729513No Hit
GCCGACTCAGCTCAGTAGGTAGAGCAACTGCTGTAGGCACCATCAATATC655330.12400133399875587No Hit
AAGTCCCCCCCCTCGCACCACTGTAGGCACCATCAATATCTCGTATGCCG648580.12272410114738085No Hit
GCCGACTTAGCTCAGTAGGTAGAGCAACTGTAGGCACCATCAATATCTCG631960.11957927003777298No Hit
TTCGCGCTGTGCGGGTTCAAGTCCCGCTCCGGGCTGTAGGCACCATCAAT617840.11690748813237808No Hit
AGCTCAGTTGGTAGAGCAGCGCACTGTAGGCACCATCAATATCTCGTATG593850.11236810796875037No Hit
TTTGCCCCGAGATGAGTTCTCCCTGACCCCTGTAGGCACCATCAATATCT583130.11033967298108513No Hit
ACGATGTCGACTTGGAGGTTGTGCCCTGTAGGCACCATCAATATCTCGTA572860.10839638685017823No Hit
TGCGCTGTGCGGGTTCAAGTCCCGCTCCGGGCTGTAGGCACCATCAATAT572430.1083150223870536No Hit
CGCTGTGCGGGTTCAAGTCCCGCTCCGGGTACCACTGTAGGCACCATCAA550200.10410866886319181No Hit
GCCGATATAGCTCAGTTGGTAGAGCAGCGCACTGTAGGCACCATCAATAT547960.10368481677621698No Hit
GGGGCTGATTCTGGATTCGACGGGATTTGCGCTGTAGGCACCATCAATAT540020.10218241250363658No Hit
AGGGCTTACGGGTTCAAGTCCCGTCCTCGGTACCACTGTAGGCACCATCA529400.10017289948413984No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)