FastQCFastQC Report
Mon 3 Jul 2023
SRR4421294_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4421294_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48812858
Total Bases2.4 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATATCTCGT9714991.9902522405059748No Hit
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA3409040.6983897562400464Illumina PCR Primer Index 6 (96% over 29bp)
ACGCTTTAGCAGCTTAATAACCTGCTTCTGTAGGCACCATCAATATCTCG2102350.43069594490861407No Hit
ACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCACTGTAGGCACCATC2041380.41820538350776343No Hit
AGCTCAGTAGGTAGAGCAACTGCTGTAGGCACCATCAATATCTCGTATGC1782080.36508413418448066No Hit
GCGAAGGTGGCGGAATTGGTAGACGCGCTAGCCTGTAGGCACCATCAATA1652460.3385296554444733No Hit
CGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCACTGTAGGCACCATCA1603390.3284769762917795No Hit
ACTTGCCCCGAGATGAGTTCTCCCTGACCCCTGTAGGCACCATCAATATC1439250.29485059039157263No Hit
ACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCCTGTAGGCACCATCAATA1419730.29085164404837754No Hit
CGGCACGTAGCGCAGCCTGGTAGCGCACCGCTGTAGGCACCATCAATATC1381140.28294594018649755No Hit
TAGGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATATCTCGT1363830.27939974340367446No Hit
CGGACGTGGGGGTTCAAGTCCCCCCCCTCGCCTGTAGGCACCATCAATAT1068010.21879685881125832No Hit
TAAGGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATATCTCG989450.2027027386923339No Hit
TACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCACTGTAGGCACCAT974050.1995478322535427No Hit
AGGCACGCTGATATGTAGGTGAAGCGACCTGTAGGCACCATCAATATCTC903500.18509467321089865No Hit
GCCGATACAGCTCAGTTGGTAGAGCAGCGCACTGTAGGCACCATCAATAT883140.18092364106195133No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGCTGTAGGCACCATCAATATC824120.16883256456731133No Hit
CGCTGTGCGGGTTCAAGTCCCGCTCCGGGCTGTAGGCACCATCAATATCT748180.15327518827109038No Hit
GCCGACTTAGCTCAGTAGGTAGAGCAACTGCTGTAGGCACCATCAATATC731710.14990107729401955No Hit
TACGCTTTAGCAGCTTAATAACCTGCTTCTGTAGGCACCATCAATATCTC668360.13692293944353762No Hit
TACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCCTGTAGGCACCATCAAT665270.1362899095152347No Hit
AAGGCTGAGGCGTGATGACGAGGCACTGTAGGCACCATCAATATCTCGTA662870.135798235784514No Hit
TCGCGCTGTGCGGGTTCAAGTCCCGCTCCGGGCTGTAGGCACCATCAATA655760.1343416523572539No Hit
GCCGAGGTGGTGGAATTGGTAGACACGCTACCCTGTAGGCACCATCAATA652020.13357546079354748No Hit
AAAGGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATATCTCG635120.13011325827305584No Hit
TATTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAAT623310.12769381378980102No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTAGGCACCATCAATAT605670.12408001186900385No Hit
AAGTCCCCCCCCTCGCACCACTGTAGGCACCATCAATATCTCGTATGCCG602290.12338757136490554No Hit
GCCGACTCAGCTCAGTAGGTAGAGCAACTGCTGTAGGCACCATCAATATC600600.12304135111285638No Hit
GCCGACTTAGCTCAGTAGGTAGAGCAACTGTAGGCACCATCAATATCTCG586100.12007082232308544No Hit
TTCGCGCTGTGCGGGTTCAAGTCCCGCTCCGGGCTGTAGGCACCATCAAT570390.11685240802740951No Hit
AGCTCAGTTGGTAGAGCAGCGCACTGTAGGCACCATCAATATCTCGTATG549080.11248675502671857No Hit
TTTGCCCCGAGATGAGTTCTCCCTGACCCCTGTAGGCACCATCAATATCT542450.11112850634560263No Hit
ACGATGTCGACTTGGAGGTTGTGCCCTGTAGGCACCATCAATATCTCGTA533460.10928677849594466No Hit
TGCGCTGTGCGGGTTCAAGTCCCGCTCCGGGCTGTAGGCACCATCAATAT526310.10782200050650588No Hit
CGCTGTGCGGGTTCAAGTCCCGCTCCGGGTACCACTGTAGGCACCATCAA509610.10440077079690765No Hit
GCCGATATAGCTCAGTTGGTAGAGCAGCGCACTGTAGGCACCATCAATAT502810.10300769522653232No Hit
GGGGCTGATTCTGGATTCGACGGGATTTGCGCTGTAGGCACCATCAATAT501300.1026983505042872No Hit
TCCTCTGCAGTTCAGTCGGTAGAACGGCGGCTGTAGGCACCATCAATATC489920.10036699756445319No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)