FastQCFastQC Report
Mon 3 Jul 2023
SRR4421295_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4421295_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57445275
Total Bases2.8 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA2814220.4898958182374442Illumina PCR Primer Index 6 (96% over 29bp)
ATGTACACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAATATCTC2347860.4087124659077705No Hit
AAGGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATATCTCGT2042210.35550530483142434No Hit
ACGCTTTAGCAGCTTAATAACCTGCTTCTGTAGGCACCATCAATATCTCG1736740.3023294779248598No Hit
ACTTGCCCCGAGATGAGTTCTCCCTGACCCCTGTAGGCACCATCAATATC1560490.2716481033470551No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTAGGCACCATCAATAT1405870.24473205150467117No Hit
ACGATGTCGACTTGGAGGTTGTGCCCTGTAGGCACCATCAATATCTCGTA1259220.21920340706872757No Hit
GCGAAGGTGGCGGAATTGGTAGACGCGCTAGCCTGTAGGCACCATCAATA1160700.20205317147493854No Hit
ACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCACTGTAGGCACCATC1101010.191662412617922No Hit
AGCTCAGTAGGTAGAGCAACTGCTGTAGGCACCATCAATATCTCGTATGC1036720.1804708916442649No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGCTGTAGGCACCATCAATATC953940.16606065511915472No Hit
CGGCACGTAGCGCAGCCTGGTAGCGCACCGCTGTAGGCACCATCAATATC918530.15989652760823236No Hit
CGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCACTGTAGGCACCATCA900890.15682577896963676No Hit
AAGCAGTGGGAGCACGCCTGTAGGCACCATCAATATCTCGTATGCCGTCT814500.1417871182616847TruSeq Adapter, Index 6 (95% over 21bp)
AGGCACGCTGATATGTAGGTGAAGCGACCTGTAGGCACCATCAATATCTC805370.14019777953887416No Hit
ACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCCTGTAGGCACCATCAATA789250.13739163055621198No Hit
ACGGTCCTAAGGTAGCGCTGTAGGCACCATCAATATCTCGTATGCCGTCT786560.13692335879669826Illumina PCR Primer Index 6 (95% over 21bp)
ATCACGGGAGACACACGGCGGGTGCTGTAGGCACCATCAATATCTCGTAT784290.1365282001000082No Hit
GCCGACTTAGCTCAGTAGGTAGAGCAACTGCTGTAGGCACCATCAATATC748180.1302422174843797No Hit
TACGCTTTAGCAGCTTAATAACCTGCTTCTGTAGGCACCATCAATATCTC715970.12463514188068557No Hit
TTTGCCCCGAGATGAGTTCTCCCTGACCCCTGTAGGCACCATCAATATCT649880.11313027920921259No Hit
ACGTGGGGGTTCAAGTCCCCCCCCTCGCACCACTGTAGGCACCATCAATA643930.1120945108192101No Hit
TACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCACTGTAGGCACCAT641470.11166627716552842No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGACCTGTAGGCACCATCAATA621780.10823866714886474No Hit
CGGACGTGGGGGTTCAAGTCCCCCCCCTCGCCTGTAGGCACCATCAATAT613570.1068094808493823No Hit
TCCTCTGCAGTTCAGTCGGTAGAACGGCGGACTGTAGGCACCATCAATAT605260.10536288667779899No Hit
AAGTCCCCCCCCTCGCACCACTGTAGGCACCATCAATATCTCGTATGCCG603590.10507217521371426No Hit
TAGGCGTGTGACTGCGTACCTTTTGCTGTAGGCACCATCAATATCTCGTA587460.10226428544384197No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)