FastQCFastQC Report
Mon 3 Jul 2023
SRR4421296_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4421296_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences65610153
Total Bases3.2 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTACACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAATATCTC3645652255.565366537096786No Hit
ATGTACACGGAGTCGACCCGCAACGCGCTGTAGGCACCATCAATATCTCG16376062.4959643060122114No Hit
TGTACACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAATATCTCG5783100.8814337012748622No Hit
ACACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAATATCTCGTAT4555180.6942797405151608No Hit
TACACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAATATCTCGTA4351790.6632799652212364No Hit
ATGTACACGGAGTCGACCCGCAACGCCTGTAGGCACCATCAATATCTCGT4194880.6393644593390904No Hit
ATGTACACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAATAACTC3845850.5861669001137675No Hit
ATGTACACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAATACCTC2972760.4530945081015129No Hit
ACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAATATCTCGTATGC2772770.42261294528607485No Hit
ATGTACACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAATAGCTC2686610.4094808314194908No Hit
TATGTACACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAATATCT2586750.3942606260954764No Hit
ATGTACACGGAGTCGACCCGCAACGCTGTAGGCACCATCAATATCTCGTA1983290.3022840077815395No Hit
ATGTACACGGAGTCGACCCGCAACCTGTAGGCACCATCAATATCTCGTAT1724260.2628038376926205No Hit
ATGTACACGGAGTCGACCCGCCTGTAGGCACCATCAATATCTCGTATGCC1642270.25030729618935654No Hit
ATGTACACGGAGTCGACCCGCAACTGTAGGCACCATCAATATCTCGTATG1620500.24698921217269526No Hit
ATGTACACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAATATATC1584870.24155865022902784No Hit
AATGTACACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAATATCT1483490.226106773444043No Hit
CGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAATATCTCGTATGCC1446480.22046587820028404No Hit
ATGTACACGGAGTCGACCCGCAACGCGACTGTAGCACCATCAATATCTCG1409490.21482803126522204No Hit
ATGTACACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCATATCTCG1385090.21110909465490807No Hit
ATGTACACGGAGTCGACCCGCACTGTAGGCACCATCAATATCTCGTATGC1368000.20850431487333979No Hit
GTCGACCCGCAACGCGACTGTAGGCACCATCAATATCTCGTATGCCGTCT1323910.20178431835085037Illumina PCR Primer Index 6 (95% over 21bp)
ATGTACACGGAGTCGACCCGCACGCGACTGTAGGCACCATCAATATCTCG1267320.19315912889274928No Hit
GTACACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAATATCTCGT1134330.1728894002121897No Hit
GAGTCGACCCGCAACGCGACTGTAGGCACCATCAATATCTCGTATGCCGT1103110.16813099033620604No Hit
AGTCGACCCGCAACGCGACTGTAGGCACCATCAATATCTCGTATGCCGTC1063950.16216240190752185No Hit
ATGTACACGGAGTCGACCGCAACGCGACTGTAGGCACCATCAATATCTCG1048070.15974204480212081No Hit
ATGTACACGGAGTCGACCCGCAACGCACTGTAGGCACCATCAATATCTCG1045790.15939453761066524No Hit
TGTCGACCCGCAACGCGACTGTAGGCACCATCAATATCTCGTATGCCGTC1035570.15783685186650911No Hit
ATGTACACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAAATCTCG988950.1507312442938519No Hit
NTGTACACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAATATCTC928430.14150706217679448No Hit
ATGTACACGGAGTCGACCCCTGTAGGCACCATCAATATCTCGTATGCCGT769240.11724404910319292No Hit
ATGTACACGGAGTCGACCCGCAACGCGACGTAGGCACCATCAATATCTCG761630.11608416764399254No Hit
ATGTACACGGAGTCGACCCGCAACGGACTGTAGGCACCATCAATATCTCG750820.11443655679327558No Hit
ATGTACACGAGTCGACCCGCAACGCGACTGTAGGCACCATCAATATCTCG724980.11049814195677916No Hit
AGTACACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAATATCTCG696780.10620002669403926No Hit
TCGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAATATCTCGTATGC656760.10010036099138497No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)