FastQCFastQC Report
Mon 3 Jul 2023
SRR4421299.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4421299.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20984390
Total Bases1 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA392011318.681091039577513Illumina Single End Adapter 1 (95% over 22bp)
ACCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA13365276.369148686237723Illumina Single End Adapter 1 (95% over 22bp)
CCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA7384273.518934789145646Illumina Single End Adapter 1 (95% over 22bp)
AGAACTCTGAACCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTG3100041.4773076558336935Illumina Single End Adapter 1 (95% over 22bp)
AAGGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATTCGTATG1948940.9287570427350997No Hit
CTCTGAACCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAA1728690.8237980708517142Illumina Single End Adapter 1 (95% over 22bp)
AACTCTGAACCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAA979760.46689944287158214Illumina Single End Adapter 1 (95% over 22bp)
GACTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA895320.4266600077486169Illumina Single End Adapter 1 (95% over 22bp)
TGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA710550.3386088420964345Illumina Single End Adapter 1 (95% over 22bp)
GATCGTCGGACTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAA669850.31921347249074195Illumina Single End Adapter 1 (95% over 22bp)
TCGTCGGACTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAA544930.2596835075977905Illumina Single End Adapter 1 (95% over 22bp)
CTGAACCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAA532900.25395067476347893Illumina Single End Adapter 1 (95% over 22bp)
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAT461640.21999209888874538Illumina Single End Adapter 1 (95% over 22bp)
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAG430960.20537170725477366Illumina Single End Adapter 1 (95% over 22bp)
AAATTGAAGAGTTTGATCATGGCTCCTGTAGGCACCATCAATTCGTATGC409630.19520700863832594No Hit
AGCGCGGTGGTCCCACCTGACCCCCTGTAGGCACCATCAATTCGTATGCC407160.1940299432101672No Hit
GCGAAGGTGGCGGAATTGGTAGACGCGCCTGTAGGCACCATCAATTCGTA404410.19271944526383658No Hit
ATCAGGTAACATCAAATCGTACCCCCTGTAGGCACCATCAATTCGTATGC372110.17732705120329922No Hit
TTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA349200.16640941194859607Illumina Single End Adapter 1 (95% over 22bp)
ACCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTTAAAAAAAAAA322780.15381910076966734Illumina Single End Adapter 1 (95% over 21bp)
ACTCTGAACCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAA313590.14943965490538444Illumina Single End Adapter 1 (95% over 22bp)
GAGAGTAGGGAACTGCCAGGCATCTGTAGGCACCATCAATTCGTATGCCG312070.14871530694959442No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAC300300.14310637573929955Illumina Single End Adapter 1 (95% over 22bp)
TCTGAACCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAA298990.14248210217213844Illumina Single End Adapter 1 (95% over 22bp)
AGGTAACATCAAATCGTACCCCAAACCGCTGTAGGCACCATCAATTCGTA296690.14138604934429833No Hit
ATGACGAGGCACCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTG294750.14046155261125054Illumina Single End Adapter 1 (95% over 22bp)
CGGACTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAA276450.13174078445930523Illumina Single End Adapter 1 (95% over 22bp)
GGGGCTGATTCTGGATTCGACGGCTGTAGGCACCATCAATTCGTATGCCG276170.13160735194113338No Hit
AGGGGTTCGAATCCCCTAGGGGACGCCACTGTAGGCACCATCAATTCGTA272600.12990608733444242No Hit
ACGATGTCGACTTGGAGGTTGTGCCCTGTAGGCACCATCAATTCGTATGC257030.12248628623467255No Hit
TGATGACGAGGCACCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCT247950.11815926028824283No Hit
AGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCCTGTAGGCACCATCA246250.11734913428505665No Hit
ACTAATGAACCGTGAGGCTTAACCCTGTAGGCACCATCAATTCGTATGCC245550.11701555298962706No Hit
CGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA242400.11551443716019383Illumina Single End Adapter 1 (95% over 22bp)
AGACCGCCAGCTAAGGTCCCAAAGTCATGGCTGTAGGCACCATCAATTCG235190.11207854981726893No Hit
GTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAA220000.10483983570644656Illumina Single End Adapter 1 (95% over 22bp)
ACGATGTCGACTTGGAGGTTGTGCCCCTGTAGGCACCATCAATTCGTATG214110.10203298737776031No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)