FastQCFastQC Report
Mon 3 Jul 2023
SRR4421300.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4421300.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18481478
Total Bases942.5 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA370642020.05478133296482Illumina Single End Adapter 1 (95% over 22bp)
ACCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA15561958.420295173362218Illumina Single End Adapter 1 (95% over 22bp)
CCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA6390853.4579756013020173Illumina Single End Adapter 1 (95% over 22bp)
AAGGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATTCGTATG1873821.0138907721557768No Hit
GACTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA1018730.5512167371029525Illumina Single End Adapter 1 (95% over 22bp)
AGCGCGGTGGTCCCACCTGACCCCCTGTAGGCACCATCAATTCGTATGCC863680.4673219317199631No Hit
CTCTGAACCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAA815770.4413986803436392Illumina Single End Adapter 1 (95% over 22bp)
AGAACTCTGAACCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTG714070.3863706138654062Illumina Single End Adapter 1 (95% over 22bp)
TGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA677460.36656159209777484Illumina Single End Adapter 1 (95% over 22bp)
GATCGTCGGACTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAA569050.3079028636129643Illumina Single End Adapter 1 (95% over 22bp)
AGGGGTTCGAATCCCCTAGGGGACGCCACTGTAGGCACCATCAATTCGTA536110.29007961376249236No Hit
AAATTGAAGAGTTTGATCATGGCTCCTGTAGGCACCATCAATTCGTATGC489580.2649030559136017No Hit
TAGTGTGGGGTCTCCCCATGCGAGAGTCTGTAGGCACCATCAATTCGTAT434190.2349325091856831No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAT420660.22761166612323974Illumina Single End Adapter 1 (95% over 22bp)
TTGCCTGGCGGCCGTAGCGCGGTGGTCCCCTGTAGGCACCATCAATTCGT397200.21491787615687447No Hit
TTGCCTGGCGGCAGTAGCGCGGTGGTCCCCTGTAGGCACCATCAATTCGT393380.21285094189977663No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAG391280.21171466914063908Illumina Single End Adapter 1 (95% over 22bp)
ACCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTTAAAAAAAAAA387620.2097343080461422Illumina Single End Adapter 1 (95% over 21bp)
TAGGCGTGTGACTGCGTACCTTTTGTCTGTAGGCACCATCAATTCGTATG380430.2058439265517617No Hit
TAAGGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATTCGTAT379270.20521627112290475No Hit
CTGAACCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAA371240.2008713805248693Illumina Single End Adapter 1 (95% over 22bp)
TGAGAGTAGGGAACTGCCAGGCATCTGTAGGCACCATCAATTCGTATGCC363660.19676997694664894No Hit
AACTCTGAACCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAA357790.19359382404372638Illumina Single End Adapter 1 (95% over 22bp)
AAAATTGAAGAGTTTGATCATGGCTCCTGTAGGCACCATCAATTCGTATG356520.19290664956558126No Hit
TTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA335490.18152768950621806Illumina Single End Adapter 1 (95% over 22bp)
AGAAGTGAAACGCCGTAGCGCCGATGGTCTGTAGGCACCATCAATTCGTA312930.1693208735794832No Hit
ATCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA286130.15481986884382298Illumina Single End Adapter 1 (95% over 22bp)
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAC278820.15086455747749178Illumina Single End Adapter 1 (95% over 22bp)
GAGAGTAGGGAACTGCCAGGCATCTGTAGGCACCATCAATTCGTATGCCG262530.1420503273601819No Hit
TTGAGGCGTGGCTTCCGGAGCTAACGCGTTCTGTAGGCACCATCAATTCG261000.14122247149281025No Hit
ATCAGGTAACATCAAATCGTACCCCCTGTAGGCACCATCAATTCGTATGC256580.13883088787595882No Hit
AAGTCGAACGGTAACAGGAAGAAGCCTGTAGGCACCATCAATTCGTATGC254970.1379597454272867No Hit
GTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAA244280.1321755760010103Illumina Single End Adapter 1 (95% over 22bp)
AGTAGGTAGCTTAACCTTCGGGAGGGCTGTAGGCACCATCAATTCGTATG242410.13116375216311163No Hit
GCGAAGGTGGCGGAATTGGTAGACGCGCCTGTAGGCACCATCAATTCGTA240810.13029802053710204No Hit
AAGTAGGTAGCTTAACCTTCGGGAGGGCTGTAGGCACCATCAATTCGTAT221780.1200012250102508No Hit
AGATTGAACGCTGGCGGCAGGCCTAACCTGTAGGCACCATCAATTCGTAT217480.1176745712653501No Hit
CGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA217470.11766916044268755Illumina Single End Adapter 1 (95% over 22bp)
ACTAATGAACCGTGAGGCTTAACCCTGTAGGCACCATCAATTCGTATGCC209460.11333509148997715No Hit
TGCCTGGCGGCCGTAGCGCGGTGGTCCCCTGTAGGCACCATCAATTCGTA203120.10990462992191426No Hit
TCCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA194890.10545152287062755Illumina Single End Adapter 1 (95% over 22bp)
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAATA193490.10469400769786917Illumina Single End Adapter 1 (95% over 22bp)
ACGATGTCGACTTGGAGGTTGTGCCCCTGTAGGCACCATCAATTCGTATG187330.10136094093773236No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)