FastQCFastQC Report
Thu 28 Sept 2023
SRR4436398_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4436398_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences87202504
Total Bases6.5 Gbp
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAA28307743.2462072419388326Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGA21268832.439015971376235Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGA20044632.2986300943835283Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAAC17610272.0194683859078175Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTG16189941.856591182289903Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAGATCGGAAGAGCACACG15089281.730372329675304No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT12374471.419049847467683No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATG11961191.3716567129769577TruSeq Adapter, Index 10 (100% over 49bp)
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGT10552051.2100627293913486No Hit
GACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGAA8845551.014368807574608Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GACGTACGCAGTTTTATCCGGTAAAGCGAGATCGGAAGAGCACACGTCTG8703140.9980378545093155Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGACGTACGCAGTTTTATCCGGTAAAGCGAGATCGGAAGAGCACACGTCT7714240.8846351476329166Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGATAGATCGGAAGAGCACAC6974130.7997625847991704No Hit
GGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGAGATCGGAAGAGCACACG6804160.7802711720296472No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTC6200730.7110724710382169No Hit
GACGTACGCAGTTTTATCCGGTAAAGCGAAGATCGGAAGAGCACACGTCT5642440.6470502269063283Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATCGGAAGAGCACA5002710.5736888014133172No Hit
GACGTACGCAGTTTTATCCGGTAAAGCAGATCGGAAGAGCACACGTCTGA3363060.3856609438646395Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGACGTACGCAGTTTTATCCGGTAAAGCAGATCGGAAGAGCACACGTCTG2641980.3029706578150554Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGACGTACGCAGTTTTATCCGGTAAAGCGAAGATCGGAAGAGCACACGTC2612420.2995808468986166No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCAGATCGGAAGAGCACAC2575150.29530688705911473No Hit
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGAGATCGGAAGAGCACACGT2513220.2882050267730844No Hit
GGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCAGATCGGAAGAGCACAC2445490.28043804797165No Hit
TTCACTGACCCGGTGAGGCGGGGAGATCGGAAGAGCACACGTCTGAACTC2205630.2529319570915074Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACGT2022920.23197957710021722No Hit
CGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCTG1993370.22859091293983944Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCGACGTACGCAGTTTTATCCGGTAAAGCGAGATCGGAAGAGCACACGTC1899290.2178022319175605No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACG1856790.21292851865813398No Hit
TTTCACTGACCCGGTGAGGCGGGGAGATCGGAAGAGCACACGTCTGAACT1844210.21148589953334365Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ACTGACCCGGTGAGGCGGGGAGATCGGAAGAGCACACGTCTGAACTCCAG1782490.20440812112459522Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CCGACGTACGCAGTTTTATCCGGTAAAGCAGATCGGAAGAGCACACGTCT1780370.20416500883965444Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GACTGCATAATTTGTGGTAGTGGGAGATCGGAAGAGCACACGTCTGAACT1676900.1922995238760575Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GACGAAGCCGAGCGCACGGGGTCGGCGGCGAAGATCGGAAGAGCACACGT1662610.19066080946482913No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGT1614120.18510018932483865No Hit
CCCGGGGAGCCCGGCGGGTGCCGGCGCGGAGATCGGAAGAGCACACGTCT1577510.18090191538536554Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTTTCACTGACCCGGTGAGGCGGGGAGATCGGAAGAGCACACGTCTGAAC1454720.16682089771183634Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAACT1449080.1661741272934089Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGACTGCATAATTTGTGGTAGTGGGAGATCGGAAGAGCACACGTCTGAAC1444870.16569134299171043Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGGGACCGGGGTCCGGTGCGGAGAGCCGTTCAGATCGGAAGAGCACACGT1380140.15826839100858847No Hit
GACCGGGGTCCGGTGCGGAGAGCCGTTCAGATCGGAAGAGCACACGTCTG1337530.15338206343249042Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGAGATCGGAAGAGCACA1328230.1523155802957218No Hit
GTCGCGGCGTGGGAAATGTGGCGTACGAGATCGGAAGAGCACACGTCTGA1305900.14975487401141602Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCGACTGCATAATTTGTGGTAGTGGGAGATCGGAAGAGCACACGTCTGAA1279760.1467572536678534Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGAAC1229960.14104640848386646Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGGCCGCCGGTGAAATACCACTACTCTCATAGATCGGAAGAGCACACGTC1227490.1407631597367892No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTC1222600.14020239602293988No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATCGGAAGAGCACAC1191180.13659928847914735No Hit
CTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGAACT1157520.1327393075776815Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GACGTACGCAGTTTTATCCGGTAAAGAGATCGGAAGAGCACACGTCTGAA1145420.13135173274382123Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGGGACCGGGGTCCGGTGCGGAGAGCCGTTAGATCGGAAGAGCACACGTC1116930.12808462472591384No Hit
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGAGATCGGAAGAGCACACGTC1089820.1249757690444302No Hit
GACGAAGCCGAGCGCACGGGGTCGGCGGCGATAGATCGGAAGAGCACACG1078680.12369828279242989No Hit
AGGGCTTCTGTCGTGAGTGGCACACGCAGAGATCGGAAGAGCACACGTCT1024320.11746451684460804Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACAC1001820.11488431570726455No Hit
GACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCTGA982670.11268827785037Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGAGATCGGAAGAGCACACG965860.11076058091175912No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCT944760.10834092562296146Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACGGACCAAGGAGTCTAACGCGTGCGCGAGTCAGATCGGAAGAGCACACG935660.10729737760741366No Hit
GTCTAGGGGTATGATTCTCGCTTAGATCGGAAGAGCACACGTCTGAACTC923140.10586163901898964Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTAGATCGGAAGAGCACAC922460.10578365960683882No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCAGATCGGAAGAGCACACGTCT921770.10570453343862694Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)