FastQCFastQC Report
Thu 28 Sept 2023
SRR4436402_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4436402_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences86677747
Total Bases6.5 Gbp
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATG1770006620.42054231058867TruSeq Adapter, Index 9 (100% over 49bp)
CTCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCT38605824.453948255023288Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCAGATCGGAAGAGCAC33835033.9035428551228954No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTG18553252.140485954255364Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCT14886371.7174385024105436Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCGACGTACGCAGTTTTATCCGGTAAAGCAGATCGGAAGAGCACACGTCT7415580.8555344660723588Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGAGATCGGAAGAGCACA6143860.7088163009128514No Hit
CCGACGTACGCAGTTTTATCCGGTAAAGCGAGATCGGAAGAGCACACGTC6122130.7063093137388539No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATCGGAAGAGCACA5952570.6867471993705605No Hit
CGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCTG5519150.6367435923317204Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGA5411210.6242905690661296Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGGCCGCCCCTCCCACGGCGCAGATCGGAAGAGCACACGTCTGAACTCCA5393030.622193144914115Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGACGTACGCAGTTTTATCCGGTAAAGCGAGATCGGAAGAGCACACGTCT5130050.591853177725074Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTG4695260.541691513970708Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTC4393650.5068948088832996No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACGT4111300.4743201274024808No Hit
GGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCAGATCGGAAGAGCACAC3762840.43411834412355No Hit
GACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGAA3748120.4324200997056373Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCGACGTACGCAGTTTTATCCGGTAAAGAGATCGGAAGAGCACACGTCTG3698800.4267300579467069Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCTGA3131720.3613061147055426Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATCGGAAGAGCAC3006520.34686180756405677No Hit
CCGACGTACGCAGTTTTATCCGGTAAAAGATCGGAAGAGCACACGTCTGA2820470.3253972441161859Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGGAGACCGGGGTTCGATTCCCCGACGGGAGATCGGAAGAGCACACGTCT2793200.3222511078881642Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC2586620.2984180011047126TruSeq Adapter, Index 9 (100% over 50bp)
CGACGTACGCAGTTTTATCCGGTAAAGCAGATCGGAAGAGCACACGTCTG2373550.27383614389515687Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCCGAGATCGGAAGAGCACA2351960.271345308502308No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCCGAGATCGGAAGAGCAC2154480.2485620674935171No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCC2148540.24787677049335397TruSeq Adapter, Index 9 (100% over 50bp)
AATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCTGAACTCCAGT2123950.24503982550446313Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAAGAGCACACGTC2068430.2386344905803793No Hit
CGACGTACGCAGTTTTATCCGGTAAAGCGAAGATCGGAAGAGCACACGTC1974040.22774472899024478No Hit
TCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGA1947410.22467242947604532Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACG1911000.22047181267874902No Hit
GTGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCAGATCGGAAGAGCA1883180.2172622230247863No Hit
GTCTAGGGGTATGATTCTCGCTTAGATCGGAAGAGCACACGTCTGAACTC1878330.21670267917785171Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CCGACGTACGCAGTTTTATCCGGTAAAGATCGGAAGAGCACACGTCTGAA1877020.21655154465424672Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GACGTACGCAGTTTTATCCGGTAAAGCGAGATCGGAAGAGCACACGTCTG1842400.21255743991592213Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCCGACGTACGCAGTTTTATCCGGTAAAGCAGATCGGAAGAGCACACGTC1823540.2103815642554715No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATATCGTATG1783460.20575754005235045TruSeq Adapter, Index 9 (97% over 49bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACAC1768420.2040223772775266No Hit
CGGGAGACCGGGGTTCGATTCCCCGACGGGAGATCGGAAGAGCACACGTC1734330.20008941856783613No Hit
GACGTACGCAGTTTTATCCGGTAAAGCAGATCGGAAGAGCACACGTCTGA1545130.17826144004412112Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTAGATCGGAAGAGCACAC1460730.16852422340880643No Hit
AATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC1436660.16574727074989618TruSeq Adapter, Index 9 (98% over 50bp)
CGCGTCTCTCGGGGTCGGGGGTGCGTGGCGGAGATCGGAAGAGCACACGT1426760.1646051090829576No Hit
GGGTTCGATTCCCCGACGGGAGATCGGAAGAGCACACGTCTGAACTCCAG1392990.16070906873017823Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGAGATCGGAAGAGCA1381090.159336167332545No Hit
TCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCG1335660.15409491434981576TruSeq Adapter, Index 9 (100% over 50bp)
GTCTAGCGGTTAGGATTCCTGGAGATCGGAAGAGCACACGTCTGAACTCC1319280.1522051559554265Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CCCGACGTACGCAGTTTTATCCGGTAAAGAGATCGGAAGAGCACACGTCT1238430.1428775023420948Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT1218830.1406162529812871No Hit
CCCGACGTACGCAGTTTTATCCGGTAAAGATCGGAAGAGCACACGTCTGA1216580.14035667078425562Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAAGAGCACACGT1205130.13903568582602868No Hit
GGGTTCGATTCCCCGACGGGGAGATCGGAAGAGCACACGTCTGAACTCCA1158840.13369521475910073Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GACGTACGCAGTTTTATCCGGTAAAGCGAAGATCGGAAGAGCACACGTCT1115480.12869277739764048Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCCGACGTACGCAGTTTTATCCGGTAAAAGATCGGAAGAGCACACGTCTG1071550.12362457921293224Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGACTGCATAATTTGTGGTAGTGGGGGAGCAGATCGGAAGAGCACACGTC1056510.1218894164381084No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGAGATCGGAAGAGCACACG1003620.11578750425988807No Hit
CTCGCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGTCT977480.11277173597970885Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGAACT969780.11188338801653439Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CCTCCCCTCTTCCCCGCGGGGCCCCGTCGTAGATCGGAAGAGCACACGTC944620.10898068220439555No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGAGAGATCGGAAGAGCACACGTC922270.10640216571388271No Hit
CGACGTACGCAGTTTTATCCGGTAAAGAGATCGGAAGAGCACACGTCTGA901210.10397247634966791Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)