FastQCFastQC Report
Thu 28 Sept 2023
SRR4436412_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4436412_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58014678
Total Bases4.3 Gbp
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATG50170918.647968364143985TruSeq Adapter, Index 8 (100% over 49bp)
TCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTG33113355.7077538205072855Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCT16445282.8346757349924445Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGA12572682.16715500859972Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCT8524871.46943330444754Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGACGTACGCAGTTTTATCCGGTAAAGCGAGATCGGAAGAGCACACGTCT7305251.259207195806551Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGAA7193451.2399362106258696Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACAC6015181.0368376085789877No Hit
CCGACGTACGCAGTTTTATCCGGTAAAGCAGATCGGAAGAGCACACGTCT4714190.8125857390779623Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCAGATCGGAAGAGCAC4633200.7986254788831199No Hit
CCGACGTACGCAGTTTTATCCGGTAAAGCGAGATCGGAAGAGCACACGTC4576000.7887659050697481No Hit
CGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCTG4483210.7727716768504688Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGA3767300.6493701473271989Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATCGGAAGAGCACA3575680.6163405750524031No Hit
GACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCTGA3548980.6117382914716858Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGACGTACGCAGTTTTATCCGGTAAAGCAGATCGGAAGAGCACACGTCTG2774210.47819105365025033Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTG2735500.4715186043090681Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GACGTACGCAGTTTTATCCGGTAAAGCGAGATCGGAAGAGCACACGTCTG2603750.448808834205716Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAGATCGGAAGAGCACACG2600830.4483055133047537No Hit
CGACGTACGCAGTTTTATCCGGTAAAGCGAAGATCGGAAGAGCACACGTC2380630.4103496015267033No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT1954280.3368595788810549No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTGGTATG1903200.3280549105176452TruSeq Adapter, Index 8 (97% over 49bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGGAAGAGCACA1871540.3225976708859782No Hit
TAAACGATGCCGACTGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTC1866140.321666871959541No Hit
ATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGAACT1791030.30872014837348577Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CCGACGTACGCAGTTTTATCCGGTAAAGAGATCGGAAGAGCACACGTCTG1747870.30128065176885066Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCGACTGCATAATTTGTGGTAGTGGGGGAGAGATCGGAAGAGCACACGTC1641780.2829939002677908No Hit
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGAGATCGGAAGAGCACA1579390.2722397252640099No Hit
GACGTACGCAGTTTTATCCGGTAAAGCGAAGATCGGAAGAGCACACGTCT1506020.25959292577647336Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGACTGCATAATTTGTGGTAGTGGGGGAGCAGATCGGAAGAGCACACGTC1470330.25344103435340964No Hit
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATCGGAAGAGCAC1278840.2204338702009171No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGAGCAGATCGGAAGAGCACACGT1233990.21270306800634142No Hit
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGATAGATCGGAAGAGCACAC1160390.2000166233793455No Hit
AATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTC1123100.19358893968178192Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCAGATCGGAAGAGCACAC1107880.19096546567060152No Hit
GACTGCATAATTTGTGGTAGTGGGGGAGCAGATCGGAAGAGCACACGTCT1066100.18376383990272255Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG1031280.1777619105289182No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTC1021170.1760192480944219No Hit
CGACTGCATAATTTGTGGTAGTGGGGGAGAGATCGGAAGAGCACACGTCT1015550.17505052773024096Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAACGATGCCGACTGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTCT973260.16776099317486515Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGT919350.15846851722593375No Hit
GGGCCGCCCCTCCCACGGCGCAGATCGGAAGAGCACACGTCTGAACTCCA878990.15151165710167347Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GTCTAGCGGTTAGGATTCCTGGAGATCGGAAGAGCACACGTCTGAACTCC877650.1512806810717798Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGC869530.14988103527869273TruSeq Adapter, Index 8 (100% over 50bp)
AATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCTGAACTCCAGT819800.14130906664689236Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GACGTACGCAGTTTTATCCGGTAAAGCAGATCGGAAGAGCACACGTCTGA806580.13903033297883682Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAAGAGCACACGTC800450.13797370382715907No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGT788700.1359483543113003No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCC765610.13196832704992348TruSeq Adapter, Index 8 (100% over 50bp)
CGCGTCTCTCGGGGTCGGGGGTGCGTGGCGGAGATCGGAAGAGCACACGT741060.12773663933806545No Hit
CCGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAGATCGGAAGAGCACAC712430.12280168132623266No Hit
ATAAACGATGCCGACTGGCGATGCGGCGGCGAGATCGGAAGAGCACACGT684440.1179770402241998No Hit
AGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACAC660080.11377810284493865No Hit
CCGACGTACGCAGTTTTATCCGGTAAAGCGAAGATCGGAAGAGCACACGT655910.11305931922952325No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATCGGAAGAGCACAC644970.11117358955263011No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTAGATCGGAAGAGCACAC606700.10457698308693533No Hit
CCCGACGTACGCAGTTTTATCCGGTAAAGCAGATCGGAAGAGCACACGTC602930.10392714754014494No Hit
CGTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACA596080.10274641186494217No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTC584480.10074691787481782No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)