FastQCFastQC Report
Thu 28 Sept 2023
SRR4450331_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4450331_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16282240
Total Bases814.1 Mbp
Sequences flagged as poor quality0
Sequence length50
%GC63

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGTCTCCGCCCCCCGGCCCCGCGTCCTCCCAGATCGGAAGAGCACACGT6821094.1892823100507055No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCAGATCGGAAGAGCA6234873.8292458531504265No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT4579462.812549133288786No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCT4291372.6356140187099566Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGGGCGGCGGCGGTCGGCGGGCGGCGGGGCGGAGATCGGAAGAGCACACG4099862.517995067017806No Hit
CGGGCGGCGGCGGTCGGCGGGCGGCGGGGCGAGATCGGAAGAGCACACGT3035181.8641046932117447No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCT3028871.8602293050587633Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTG2419241.4858152195275343Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCAGATCGGAAGAGCAC1751041.0754294249439882No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATG1540590.9461781671317951TruSeq Adapter, Index 1 (100% over 49bp)
CTCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTC1374220.843999351440588No Hit
CGTCTCCGCCCCCCGGCCCCGCGTCCTCCCAGATCGGAAGAGCACACGTC1319320.8102816320113204No Hit
AGGGCGCGCGGGTCGGGGCGGCGGCGGCGGAGATCGGAAGAGCACACGTC1255700.7712083841043984No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGGAGATCGGAAGAGCACACG1217850.7479621968476082No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGAAGATCGGAAGAGCACA1153420.7083914743917299No Hit
TCCTCCTCCCCCGTCTCCGCCCCCCGGCCCAGATCGGAAGAGCACACGTC974070.5982407825950238No Hit
CCGTCTCCGCCCCCCGGCCCCGCGTCCTCCAGATCGGAAGAGCACACGTC913590.5610960162729453No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGAGATCGGAAGAGCACACG908510.5579760524350458No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGAGATCGGAAGAGC861540.5291286702566723No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACAGATCGGAAGAGCAC860320.5283793876026885No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTG818300.5025721276679376Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGCGGCGGCGGTCGGCGGGCGGCGGGGCGGAGATCGGAAGAGCACACGT803010.493181527848748No Hit
GAGGGCGCGCGGGTCGGGGCGGCGGCGGCGGAGATCGGAAGAGCACACGT771660.47392742030580554No Hit
CGGGCGGCGGCGGTCGGCGGGCGGCGGGGCAGATCGGAAGAGCACACGTC719740.4420399158838096No Hit
CCCGTCTCCGCCCCCCGGCCCCGCGTCCTCCCAGATCGGAAGAGCACACG717280.4405290672536457No Hit
CCGTCTCCGCCCCCCGGCCCCGCGTCCTAGATCGGAAGAGCACACGTCTG711210.43680107896702175Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGAGATCGGAAGAGCACAC654750.4021252604064306No Hit
ATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGAACT579530.35592768562556504Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACAC561850.34506922880389923No Hit
CCGTCTCCGCCCCCCGGCCCCGCGTCCTCCCTAGATCGGAAGAGCACACG538150.3305134920011006No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCAGATCGGAAGAG517160.31762214535592154No Hit
AGGGCGCGCGGGTCGGGGCGGCGGCGGCGGCAGATCGGAAGAGCACACGT501620.3080780040092764No Hit
CGGTCGGCGGGCGGCGGGGCGGGGAGATCGGAAGAGCACACGTCTGAACT499000.30646888880154083Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CCGTCTCCGCCCCCCGGCCCCGCGAGATCGGAAGAGCACACGTCTGAACT492420.3024276757989073Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CCGTCTCCGCCCCCCGGCCCCGCGTCCTCAGATCGGAAGAGCACACGTCT484830.29776615502535275Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGGAAGATCGGAAGAGCACAC468710.28786579733501044No Hit
ATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTCTGAAC454870.27936573837506384Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACG452430.2778671730670964No Hit
CCCGTCTCCGCCCCCCGGCCCCGCGAGATCGGAAGAGCACACGTCTGAAC449680.27617821626508393Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGA444550.27302754412169333Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCGTCTCCGCCCCCCGGCCCCGCGTCCAGATCGGAAGAGCACACGTCTGA429130.2635571027082269Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCTCGCTTCTGGCGCCAAGCGCCCGGCCGAGATCGGAAGAGCACACGTCT413040.2536751700011792Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCAGATCGGAAGAGCACACG406580.2497076569317244No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG399090.24510755276915216No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAAGATCGGAAGAGCACACGT392500.24106019810542037No Hit
CGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCTG392500.24106019810542037Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGGGCGGCGGCGGTCGGCGGGCGGCGGGGAGATCGGAAGAGCACACGTCT376000.23092645729334538Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTCTTGCGACCCGGGTTCGATTCCCGGGCGGCGAGATCGGAAGAGCACAC363660.22334764749813293No Hit
GGGCGGCGGCGGTCGGCGGGCGGCGGGGCGAGATCGGAAGAGCACACGTC355510.21834219370307772No Hit
CGGTCGGCGGGCGGCGGGGCGGGGCAGATCGGAAGAGCACACGTCTGAAC338110.20765570339216227Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CCCGTCTCCGCCCCCCGGCCCCGCGTCCTAGATCGGAAGAGCACACGTCT328400.20169215046578357Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAGATCGGAAGAGCACA321060.19718417121968476No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGAAGATCGGAAGAGCACAC319100.19598040564443223No Hit
TCTCCGCCCCCCGGCCCCGCGTCCTCCCAGATCGGAAGAGCACACGTCTG317710.19512671475177862Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGA317710.19512671475177862Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTCCTCCTCCCCCGTCTCCGCCCCCCGGCCCAGATCGGAAGAGCACACGT307540.1888806454148815No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCAGATCGGAAGAGCACAC298430.18328559215439644No Hit
CGGCGGCGGTCGGCGGGCGGCGGGGCGGGGAGATCGGAAGAGCACACGTC294500.18087191934279312No Hit
CGGGTCGGGGCGGCGGCGGCGGAGATCGGAAGAGCACACGTCTGAACTCC293670.18036216147164025Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGGGAGACCGGGGTTCGATTCCCCGACGGGGAAGATCGGAAGAGCACACG292590.1796988620730317No Hit
CGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTG282710.1736309009079832Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGGCGGCGGTCGGCGGGCGGCGGGGCGGAGATCGGAAGAGCACACGTCTG267190.16409904288353447Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCGTCTCCGCCCCCCGGCCCAGATCGGAAGAGCACACGTCTGAACTCCAG265990.1633620435517472Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CCGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCT253800.15587535867300814Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGGGGGGGCCGGGCCACCCCTCCCACGGCGCGAAGATCGGAAGAGCACAC248520.15263256161314415No Hit
TCCCCCGTCTCCGCCCCCCGGCCCAGATCGGAAGAGCACACGTCTGAACT245250.1506242384340238Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCAGATCGGAAGAGC245010.15047683856766636No Hit
CGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTCT244160.14995479737431705Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCCGTCTCCGCCCCCCGGCCCCGCGTCCAGATCGGAAGAGCACACGTCTG242800.14911953146495815Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCTGGTCCGAAGGTAGTGAGTTATCTCAAAGATCGGAAGAGCACACGTCT241740.14846851538854605Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACAGATCGGAAGAGCACA238430.1464356255650328No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTC234570.1440649443811171No Hit
CGGGCGGCGGCGGTCGGCGGGCGGCGGGGCGGGAGATCGGAAGAGCACAC229390.14088356393223536No Hit
CGGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAAGAGCACACGT210960.12956448252820252No Hit
GGCCACCCCTCCCACGGCGCGACCAGATCGGAAGAGCACACGTCTGAACT210140.12906086631814787Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GTCTAGGGGTATGATTCTCGCTTAGATCGGAAGAGCACACGTCTGAACTC208690.12817032545890492Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCCCACATGGTCTAGCGGTTAGGATTCCTGGAGATCGGAAGAGCACACGT208050.12777725914861837No Hit
CCGTCTCCGCCCCCCGGCCCCGCGTAGATCGGAAGAGCACACGTCTGAAC194720.11959042490468143Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGCGGCGGCGGTCGGCGGGCGGCGGGGCGGAGATCGGAAGAGCACACGTC193950.11911751700011793No Hit
ATCGTATAGTGGTTAGTACTCTGCGTTAGATCGGAAGAGCACACGTCTGA193340.11874287567312605Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCAGATCGGAAGAGCACACG187810.11534653708580636No Hit
CTAGCTCAGTCGGTAGAGCATGAGAAGATCGGAAGAGCACACGTCTGAAC187130.11492890413112693Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGGCGGCGGTCGGCGGGCGGCGGGGCGGGAGATCGGAAGAGCACACGTCT182640.11217129829802289Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGAGATCGGAAGAGCACACGT181650.11156327384929839No Hit
CGTCTCCGCCCCCCGGCCCCGCGTCCTCCCTAGATCGGAAGAGCACACGT180040.1105744664124838No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCAGATCGGAAGAGCA178390.1095610923312763No Hit
CCGGGCCACCCCTCCCACGGCGCGACCAGATCGGAAGAGCACACGTCTGA175890.10802567705671946Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACAC175240.10762646908533469No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGAGATCGGAAGAGCACACG174350.10707986124759247No Hit
CCCGTCTCCGCCCCCCGGCCCCGCGTCCTCCAGATCGGAAGAGCACACGT171390.1052619295625172No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGAGATCGGAAGAGCACACGTC164630.10111016666011557No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)