FastQCFastQC Report
Thu 28 Sept 2023
SRR4450332_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4450332_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16020153
Total Bases801 Mbp
Sequences flagged as poor quality0
Sequence length50
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATG170503910.64308811532574TruSeq Adapter, Index 5 (100% over 49bp)
CTCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCT5959763.7201642206538224Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCAGATCGGAAGAGCA3441392.1481630044357254No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCT3379012.109224549852926Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT2609411.6288296372700062No Hit
CCGTCTCCGCCCCCCGGCCCCGCGTCCTCCCAGATCGGAAGAGCACACGT2456861.5336058276097613No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTG1767851.1035163022475503Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGGAGATCGGAAGAGCACACG1578000.985009319199386No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTC1550230.9676749029800152No Hit
AGGGCGCGCGGGTCGGGGCGGCGGCGGCGGAGATCGGAAGAGCACACGTC1184030.7390878226943276No Hit
CGGGCGGCGGCGGTCGGCGGGCGGCGGGGCGGAGATCGGAAGAGCACACG1023270.6387392180336854No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTG1021610.6377030231858585Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAAGATCGGAAGAGCACACGT1002130.6255433390679852No Hit
GTCTAGGGGTATGATTCTCGCTTAGATCGGAAGAGCACACGTCTGAACTC988180.616835557063656Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGAAGATCGGAAGAGCACA976450.6095135296148545No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGAGATCGGAAGAGCACAC976080.6092825705222665No Hit
TGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCAGATCGGAAGAGCAC884530.5521358004508446No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCAGATCGGAAGAGCACACG790150.49322250542800683No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGAGATCGGAAGAGCACACG739350.46151244622944615No Hit
CGGGCGGCGGCGGTCGGCGGGCGGCGGGGCGAGATCGGAAGAGCACACGT738760.4611441601088329No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGATCGGAAGAGCACACGTCTG683200.4264628433948165Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACAGATCGGAAGAGCAC665590.415470439015158No Hit
ATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTCTGAAC647440.4041409592030738Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATATCGTATG647270.40403484286323604TruSeq Adapter, Index 5 (97% over 49bp)
CCCGTCTCCGCCCCCCGGCCCCGCGTCCAGATCGGAAGAGCACACGTCTG627150.3914756619365621Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCTG606090.37832972007196186Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAGATCGGAAGAGCACA576440.3598217819767389No Hit
CCGTCTCCGCCCCCCGGCCCCGCGTCCTAGATCGGAAGAGCACACGTCTG565660.35309275760350106Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCGTCTCCGCCCCCCGGCCCCGCGTCCAGATCGGAAGAGCACACGTCTGA553080.345240148455511Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGAACT529960.3308083262375834Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GAGGGCGCGCGGGTCGGGGCGGCGGCGGCGGAGATCGGAAGAGCACACGT507250.31663243166279376No Hit
TTCTTGCGACCCGGGTTCGATTCCCGGGCGGCGAGATCGGAAGAGCACAC483700.30193219752645306No Hit
TCTCGCTTCTGGCGCCAAGCGCCCGGCCGAGATCGGAAGAGCACACGTCT477540.29808704074174575Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGGGAGACCGGGGTTCGATTCCCCGACGGGGAAGATCGGAAGAGCACACG456480.2849410988771455No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGGAAGATCGGAAGAGCACAC456010.2846477184081825No Hit
ACCCCTCCCACGGCGCGACCAGATCGGAAGAGCACACGTCTGAACTCCAG437280.2729561946131226Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGAAGATCGGAAGAGCACACGT417640.2606966362930491No Hit
CTAGCTCAGTCGGTAGAGCATGAGAAGATCGGAAGAGCACACGTCTGAAC415070.2590924069202086Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGTCTCCGCCCCCCGGCCCCGCGTCCTCCCAGATCGGAAGAGCACACGTC413210.25793136931963134No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACAC411350.2567703317190541No Hit
CCGTCTCCGCCCCCCGGCCCCGCGTCCTCCAGATCGGAAGAGCACACGTC394350.2461586977352838No Hit
TCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGA386210.24107759769834905Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAAGAGCACACGTC381050.2378566546773929No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGAGATCGGAAGAGCACACGTC377900.23589038132157664No Hit
CGGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAAGAGCACACGT377660.23574057001827636No Hit
CCCGTCTCCGCCCCCCGGCCCCGCGTCCTCCCAGATCGGAAGAGCACACG370350.2311775674052551No Hit
CCGTCTCCGCCCCCCGGCCCCGCGAGATCGGAAGAGCACACGTCTGAACT350420.21873698709369377Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGGGAGACCGGGGTTCGATTCCCCGACGGGAGATCGGAAGAGCACACGTC350290.21865583930440616No Hit
CGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGA340640.21263217648420712Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGAGATCGGAAGAGCACACGT339410.21186439355479317No Hit
GGGAGACCGGGGTTCGATTCCCCGACGGGAGATCGGAAGAGCACACGTCT337690.21079074588114108Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGAGATCGGAAGAGCACA322040.2010218004784349No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG320800.2002477754113834No Hit
CGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTCT309150.19297568506368198Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGGGTCGGGGCGGCGGCGGCGGAGATCGGAAGAGCACACGTCTGAACTCC306430.19127782362627874Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CCGTCTCCGCCCCCCGGCCCCGCGTAGATCGGAAGAGCACACGTCTGAAC305920.1909594746067656Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGGTCGGCGGGCGGCGGGGCGGGGAGATCGGAAGAGCACACGTCTGAACT299270.18680845307782018Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GGGTCGCGCCGTCGGGCCCGGGGGAGATCGGAAGAGCACACGTCTGAACT299200.18676475811435758Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TGCGAGAGGTCCCGGGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGT295390.18438650367446555Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CCCGTCTCCGCCCCCCGGCCCCGCGTCCTAGATCGGAAGAGCACACGTCT290940.1816087524257727Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGGAGATCGGAAGAGCACACGTCT289320.18059752612849578Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCCGTCTCCGCCCCCCGGCCCAGATCGGAAGAGCACACGTCTGAACTCCA287780.17963623693231895Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCTCCTCCCCCGTCTCCGCCCCCCGGCCCAGATCGGAAGAGCACACGTC280330.17498584439237252No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCCGAGATCGGAAGAGCACACGTCTG261260.16308208791763723Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGAGACCGGGGTTCGATTCCCCGACGGGGAAGATCGGAAGAGCACACGT260640.16269507538411151No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCAGATCGGAAGAGCA255370.1594054688491427No Hit
GGGCGGCGGCGGTCGGCGGGCGGCGGGGCGGAGATCGGAAGAGCACACGT252140.15738925839222634No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC250430.1563218528562118TruSeq Adapter, Index 5 (100% over 50bp)
CCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTC249370.15566018626663553No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGAAGATCGGAAGAGCACAC246140.15364397580971917No Hit
GACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCTGA245310.15312587838580569Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTCAGTCGGTAGAGCATGAGAAGATCGGAAGAGCACACGTCTGAACTCCA245290.15311339411053065Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCCTCGGTTGGCCTCGGATAGCCGGTCCCCCGAGATCGGAAGAGCACACG241770.15091616166212643No Hit
CGGGCGGCGGCGGTCGGCGGGCGGCGGGGCAGATCGGAAGAGCACACGTC238990.14918084739889811No Hit
TCTTGCGACCCGGGTTCGATTCCCGGGCGGCGAGATCGGAAGAGCACACG235100.14675265585790598No Hit
AGGCGGCCCGGGTTCGACTCCCGGTGTGGGAGATCGGAAGAGCACACGTC234000.14606602071777966No Hit
AACGATGCCGACCGGCGATGCGGCGGCGTTATAGATCGGAAGAGCACACG231220.14433070645455134No Hit
AGGGCGCGCGGGTCGGGGCGGCGGCGGCGGCAGATCGGAAGAGCACACGT230270.1437377033789877No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACG224070.1398675780437303No Hit
AACGATGCCGACCGGCGATGCGGCGGCGTTAAGATCGGAAGAGCACACGT222830.13909355297667883No Hit
GCGGGAGACCGGGGTTCGATTCCCCGACGGGAGATCGGAAGAGCACACGT222210.13870654044315306No Hit
CCGTCTCCGCCCCCCGGCCCAGATCGGAAGAGCACACGTCTGAACTCCAG222140.1386628454796905Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CCCGTCTCCGCCCCCCGGCCCCGCGAGATCGGAAGAGCACACGTCTGAAC217150.135548018798572Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGAGATCGGAAGAGC215080.13425589630760704No Hit
CCGTCTCCGCCCCCCGGCCCCGCGTCCTCAGATCGGAAGAGCACACGTCT208300.13002372698937395Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGAGATCGGAAGAGCACACG205000.12796382156899502No Hit
TGGGGGGGCCGGGCCACCCCTCCCACGGCGCGAAGATCGGAAGAGCACAC202910.126659214802755No Hit
GACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGAA195370.121952643024071Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGAGATCGGAAGAGCAC191390.11946827224434124No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCC189120.11805130700062604TruSeq Adapter, Index 5 (100% over 50bp)
AATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCTGAACTCCAGT181640.11338218804776709Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AGGCGGCCCGGGTTCGACTCCCGGTGTGGAGATCGGAAGAGCACACGTCT181100.11304511261534143Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCCCGGATGATCCTCAGTGGTCTGGGGTGCAGGAGATCGGAAGAGCACAC180930.11293899627550373No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGAGATCGGAAGAGCACACGT179650.11214000265790221No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGAGATCGGAAGAGCACACG177640.1108853329927623No Hit
GCTGGTCCGAAGGTAGTGAGTTATCTCAAAGATCGGAAGAGCACACGTCT174150.10870682695727063Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCAGATCGGAAGAGCACAC173240.10813879243225703No Hit
AGGCGCCGGCCGAGGCGAGGCGCCGCGCGGAACAGATCGGAAGAGCACAC173130.10807012891824441No Hit
CGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTG171360.10696527055640481Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTCTAGCGGTTAGGATTCCTGGAGATCGGAAGAGCACACGTCTGAACTCC170020.1061288241129782Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)