FastQCFastQC Report
Tue 27 Jun 2023
SRR458756.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR458756.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21824511
Total Bases785.6 Mbp
Sequences flagged as poor quality0
Sequence length36
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TACTTGGATAACTGTGGTAATTCTAGAGCATCTCGT2164400.9917289784866199No Hit
TGGTGACTCTAGATAACCTCGGGCCGATCGCACATC2017530.924433083517885No Hit
CTACTTGGATAACTGTGGTAATTCTAGAGCATCTCG1776930.8141900636399139No Hit
TACTTGGATAACTGTGGTAATTCTAGAGCTATCTCG1731450.7933511087602376No Hit
CGCTGAGAAGACGGTCGAACTTGACTATCTATCTCG1548090.7093354806437587No Hit
CGCTGAGAAGACGGTCGAACTTGACTATCTAATCTC1243670.5698501102728029No Hit
TGGTGACTCTAGATAACCTCGGGCCGATCGCATCTC1104630.5061419245544608No Hit
CTACTTGGATAACTGTGGTAATTCTAGAGATCTCGT1091680.5002082291786515No Hit
CTACTTGGATAACTGTGGTAATTCTAGAGCTATCTC951410.4359364569497113No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN813760.3728651698083865No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGATCTC813430.3727139636713968No Hit
ATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA782720.35864262892304893Illumina Paired End PCR Primer 2 (100% over 24bp)
TGGATGGTTTAGTGAGGCCCTCGGATCGGCCATCTC679870.31151671622791455No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATCT654230.2997684575842272No Hit
TTGAAAAAATTAGAGTGTTCAAAGCAGGCCCATCTC628650.2880476909654471No Hit
TGAAAAAATTAGAGTGTTCAAAGCAGGCCCATCTCG626200.28692509994840204No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACAT624010.28592164103928835No Hit
ACTCTAGATAACCTCGGGCCGATCGCACATCTCGTA568050.26028074580915006No Hit
TACTTGGATAACTGTGGTAATTCTAGAGCTAATCTC550990.25246384672719585No Hit
TTTGAAAAAATTAGAGTGTTCAAAGCAGGCCCATCT501150.2296271380375945No Hit
AAAAAATTAGAGTGTTCAAAGCAGGCCCATCTCGTA483790.22167277883110417No Hit
GGCTTTGGTGACTCTAGATAACCTCGGGCATCTCGT442920.2029461278651329No Hit
GGTGACTCTAGATAACCTCGGGCCGATCGCACATCT420040.19246250236717788No Hit
GAAAAAATTAGAGTGTTCAAAGCAGGCCCATCTCGT403620.18493885155090073No Hit
GACTCTAGATAACCTCGGGCCGATCGCACATCTCGT397890.18231336317226077No Hit
ACTTGGATAACTGTGGTAATTCTAGAGCATCTCGTA393250.18018731324610204No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCATCTCG367860.1685536047061948No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCATCT363650.16662458095853785No Hit
TGGATACCGCAGCTAGGAATAATGGAATATCTCGTA363510.166560432900421No Hit
TGGTGACTCTAGATAACCTCGGGCCGATCGCAATCT362200.16596019035661327No Hit
TACTTGGATAACTGTGGTAATTCTAGAGATCTCGTA354440.16240455513527888No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCATC339610.15560944297904317No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCATCT334410.153226800820417No Hit
CTTTGAAAAAATTAGAGTGTTCAAAGCAGGCCCATC332470.1523378920150834No Hit
GGCTTTGGTGACTCTAGATAACCTCGGGCCATCTCG329970.15119239097728238No Hit
TGACTCTAGATAACCTCGGGCCGATCGCACATCTCG328200.15038137624251927No Hit
CGCTGAGAAGACGGTCGAACTTGACTATCTAGATCT309300.14172138839674348No Hit
AGCTAATACATGCCGACGGGCGCTGACCCATCTCGT289750.13276357028113941No Hit
ACTTGGATAACTGTGGTAATTCTAGAGCTATCTCGT289100.13246574001131114No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCATCT283080.12970737351228626No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCATCTC278510.12761339761518598No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAAATC271580.12443806873840152No Hit
GCTTTGGTGACTCTAGATAACCTCGGGCCATCTCGT270510.12394779429422267No Hit
GTGACTCTAGATAACCTCGGGCCGATCGCACATCTC262930.12047463514760995No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAATC258880.11861892346637228No Hit
CGCTGAGAAGACGGTCGAACTTGACTATCTAGAATC258360.11838065925050968No Hit
ACTGTGGTAATTCTAGAGCTAATACATGCCATCTCG254380.11655702159833042No Hit
CGCTGAGAAGACGGTCGAACTTGACTATCATCTCGT246060.1127447941445286No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCATCT245790.11262108003244609No Hit
CTACTTGGATAACTGTGGTAATTCTAGAGCTAATCT243750.11168635118560044No Hit
AGAGCTAATACATGCCGACGGGCGCTGACCATCTCG240340.11012388777003985No Hit
CTTGGATAACTGTGGTAATTCTAGAGCTATCTCGTA234880.10762211350348239No Hit
CTAGAGCTAATACATGCCGACGGGCGCTGACCCCAT231890.10625209426227235No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGAATCTCG226820.10392901815761188No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)