FastQCFastQC Report
Tue 27 Jun 2023
SRR458757.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR458757.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18520372
Total Bases666.7 Mbp
Sequences flagged as poor quality0
Sequence length36
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA3002411.6211391434254128Illumina Paired End PCR Primer 2 (100% over 24bp)
CGCTGAGAAGACGGTCGAACTTGACTATCTATCTCG2611261.4099392819971435No Hit
CGCTGAGAAGACGGTCGAACTTGACTATCTAATCTC1887781.0192991803836338No Hit
TACTTGGATAACTGTGGTAATTCTAGAGCATCTCGT1858371.0034193697621192No Hit
TACTTGGATAACTGTGGTAATTCTAGAGCTATCTCG1727100.9325406638700345No Hit
TGGTGACTCTAGATAACCTCGGGCCGATCGCACATC1572300.8489570295888226No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACAT1428810.7714801840913347No Hit
CTACTTGGATAACTGTGGTAATTCTAGAGCATCTCG1082020.5842323253550199No Hit
TGGTGACTCTAGATAACCTCGGGCCGATCGCATCTC896890.48427213017103543No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGATCTC830970.44867889262699473No Hit
TGGATGGTTTAGTGAGGCCCTCGGATCGGCCATCTC815170.44014774649234906No Hit
CTACTTGGATAACTGTGGTAATTCTAGAGATCTCGT703850.3800409624601493No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCATC648810.35032233693794057No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN647750.34974999422257824No Hit
CTACTTGGATAACTGTGGTAATTCTAGAGCTATCTC630530.34045212482773024No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCATCT618440.3339241781968526No Hit
CCGACGATCATCTCGTATGCCGTCTTCTGCTTGAAA577600.31187278527666723Illumina PCR Primer Index 7 (100% over 28bp)
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATCT536090.2894596285646962No Hit
ACTCTAGATAACCTCGGGCCGATCGCACATCTCGTA519060.2802643489018471No Hit
TACTTGGATAACTGTGGTAATTCTAGAGCTAATCTC518460.27994038132711374No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCATCT509750.2752374520339008No Hit
CGCTGAGAAGACGGTCGAACTTGACTATCATCTCGT489500.26430354638664927No Hit
GGTGACTCTAGATAACCTCGGGCCGATCGCACATCT482350.26044293278774316No Hit
GACTCTAGATAACCTCGGGCCGATCGCACATCTCGT458410.24751662655588128No Hit
TTGAAAAAATTAGAGTGTTCAAAGCAGGCCCATCTC448750.24230074860267384No Hit
TGAAAAAATTAGAGTGTTCAAAGCAGGCCCATCTCG436400.2356324160227451No Hit
TTTGAAAAAATTAGAGTGTTCAAAGCAGGCCCATCT422700.2282351563996663No Hit
CGCTGAGAAGACGGTCGAACTTGACTATCTAGATCT419660.22659372068768383No Hit
TGGATACCGCAGCTAGGAATAATGGAATATCTCGTA416570.22492528767780692No Hit
GGCTTTGGTGACTCTAGATAACCTCGGGCATCTCGT385420.20810597108956558No Hit
TGACTCTAGATAACCTCGGGCCGATCGCACATCTCG380030.20519566237654405No Hit
ACTTGGATAACTGTGGTAATTCTAGAGCTATCTCGT365690.19745283734041627No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCAT356410.19244213885120667No Hit
AAAAAATTAGAGTGTTCAAAGCAGGCCCATCTCGTA347960.1878795955070449No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAAATC342920.1851582678792845No Hit
ACTGTGGTAATTCTAGAGCTAATACATGCCATCTCG332630.17960222397260703No Hit
GGCTTTGGTGACTCTAGATAACCTCGGGCCATCTCG327140.1766379206637966No Hit
TGGTGACTCTAGATAACCTCGGGCCGATCGCAATCT316530.17090909405059468No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCAATC315620.1704177432289157No Hit
GCTTTGGTGACTCTAGATAACCTCGGGCCATCTCGT305700.16506147932665716No Hit
CGCTGAGAAGACGGTCGAACTTGACTATCTAGAATC304070.16418136741529815No Hit
TACTTGGATAACTGTGGTAATTCTAGAGCTAATAAT301660.16288009765678574No Hit
GTGACTCTAGATAACCTCGGGCCGATCGCACATCTC298790.1613304527576444No Hit
CGACGATCATCTCGTATGCCGTCTTCTGCTTGAAAA293420.15843094296378063Illumina PCR Primer Index 7 (100% over 28bp)
GAAAAAATTAGAGTGTTCAAAGCAGGCCCATCTCGT286840.15487809856087123No Hit
CTTTGAAAAAATTAGAGTGTTCAAAGCAGGCCCATC286410.15464592179897899No Hit
GACGATCATCTCGTATGCCGTCTTCTGCTTGAAAAA280070.15122266442596294Illumina PCR Primer Index 7 (96% over 29bp)
ACTTGGATAACTGTGGTAATTCTAGAGCATCTCGTA279220.1507637103617573No Hit
CTGGATACCGCAGCTAGGAATAATGGAATATCTCGT277730.14995919088450274No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGAATCTCG276640.14937064979040376No Hit
TACTTGGATAACTGTGGTAATTCTAGAGATCTCGTA269100.14529945726792096No Hit
TAACTGTGGTAATTCTAGAGCTAATACATGCCATCT260620.14072071554502252No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAATC257200.13887410036904227No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCATCT255740.13808577927052437No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCATCTCG255680.13805338251305102No Hit
AACTGTGGTAATTCTAGAGCTAATACATGCCATCTC247220.13348543970931037No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCATC235570.12719506929990393No Hit
TGTGGTAATTCTAGAGCTAATACATGCCATCTCGTA234810.1267847103719083No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCATCTC225210.12160122917617422No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCATCTC222420.120094779953664No Hit
ATAACTGTGGTAATTCTAGAGCTAATACATGCCATC216690.11700088961496022No Hit
ATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAC214940.11605598418865454Illumina Paired End PCR Primer 2 (100% over 24bp)
GCTGAGAAGACGGTCGAACTTGACTATCTATCTCGT197660.10672571803633317No Hit
AAATCAGTTATGGTTCCTTTGGTCGCTCATCTCGTA196680.10619657099760198No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCATCTCGTA194960.10526786395003297No Hit
CTTTGGTGACTCTAGATAACCTCGGGCATCTCGTAT190550.10288670227574263No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCATCT186750.10083490763576454No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)