FastQCFastQC Report
Fri 29 Sept 2023
SRR5013260.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5013260.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences314041138
Total Bases28.2 Gbp
Sequences flagged as poor quality0
Sequence length90
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT76569622.438203494218646No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAA62704131.996685224086788No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATT49148351.5650290376925076No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAA33538341.067960083624458No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAAT32659381.0399713938114694No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCGCTGTAGGCACCATCAA27473540.8748388881459217No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAAT26543170.8452131516604044No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATT16100350.5126828320180142No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACTGTAGGCACCATCAATTC14387970.4581555808780695No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCTGTAGGCACCATCAATT14285370.4548884929846357No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATT13703120.436347928404208No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAAT13555110.4316348516097913No Hit
CGGCGGATCTTTCCCGCCCCCCGTTCCTCCCGACCCCCTGTAGGCACCAT9892860.31501796430249845No Hit
CCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT9339900.2974100800768337No Hit
GCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT8137130.25911032076313517No Hit
GGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCGCTGTAGGCACCATCA8061930.25671573002642734No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAAT7550830.24044079218691403No Hit
CTCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCA7243980.23066977932044047No Hit
GCGCCGCTGGTGTAGTGGTATCATGCAAGATTCTGTAGGCACCATCAATT7201440.22931517972018048No Hit
ATCTGAGGGTCCAGGGTTCAGGTCCCTGTTCGCTGTAGGCACCATCAATT6475830.20620960811828415No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGACTGTAGGCACCATCAAT6393250.2035800163225749No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCCTGTAGGCACCATCAAT6227750.19831000612410213No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATTC6079100.1935765498340539No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCTGTAGGCACCATCAATTC5973540.19021520677332407No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA5964280.189920340945905Illumina Single End Adapter 1 (95% over 22bp)
TGGCCGTGATCGTATAGTGGTTAGTACTCTGCGCTGTAGGCACCATCAAT5785130.18421567431716543No Hit
TGCTCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCAT5646160.1797904578985445No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAATT5403200.1720538918694149No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCTGTAGGCACCATCAATTCGTAT5048320.16075346154171688No Hit
CCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAA5001630.15926671364947098No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCACC4938390.15725296473737782No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGACTGTAGGCACCATCAATTCGT4846810.1543367862843498No Hit
GTCTAGGGGTATGATTCTCGCTTCTGTAGGCACCATCAATTCGTATGCCG4823430.1535922978345595No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGCTGTAGGCACCATCAA4574330.14566021601921467No Hit
GCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATT4344870.1383535299760632No Hit
AGGGGGGTCTCCCCCGCGGGGGCGCGCCGGCGCTGTAGGCACCATCAATT4187750.13335036379851611No Hit
GCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAA4098630.13051251903182187No Hit
GGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCTGTAGGCACCATCAAT4050300.12897354868202013No Hit
TAGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCGCTGTAGGCACCATCAA3987670.1269792239767008No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGACTGTAGGCACCATCAATTCG3981190.12677288158343128No Hit
CGGCGGATCTTTCCCGCCCCCCGTTCCTCCCGACCCCTCTGTAGGCACCA3840250.12228493452981946No Hit
GCGTTGGTGGTATAGTGGTGAGCATAGCTGCCTGTAGGCACCATCAATTC3810200.12132805352399405No Hit
CGCGGGAGGCCCGGGTTCGTTTCCCGGCCAATGCCTGTAGGCACCATCAA3728440.11872457295706272No Hit
TAAGCCAGGGATTGTGGGTTCGGGTCCCACCTGGCTGTAGGCACCATCAA3577390.11391469355839616No Hit
TAAGCCAGGGATTGTGGGTTCGTGTCCCACCTGGCTGTAGGCACCATCAA3569790.11367268704777142No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATTC3507860.11170065241579911No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCGCCTGTAGGCACCATCA3472120.11056258495662438No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTGTAGGCACCATCAATTC3356820.1068910914467518No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGCTGTAGGCACCATCAATTCGT3324410.105859061050785No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCCTGTAGGCACCATCAAT3313040.10549700657370563No Hit
AGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCACCA3295310.10493243085878767No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCCTGTAGGCACCAT3253810.10361094793892894No Hit
TCGGCGGATCTTTCCCGCCCCCCGTTCCTCCCGACCCCCTGTAGGCACCA3245850.10335747796201146No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)